一次性牙刷上脱落细胞的DNA提取及STR分型

发布时间：2018-01-10 14:16

本文关键词：一次性牙刷上脱落细胞的DNA提取及STR分型　出处：《中国法医学杂志》2011年06期 　论文类型：期刊论文

【摘要】：目的研究一次性使用牙刷上脱落细胞的DNA提取和STR分型。方法对一次性使用牙刷的采集方法、采集部位、DNA提取方法、存放时间对STR分型的影响进行比对研究。结果割取法可获得较高浓度的DNA,30例中检出9个以上基因座达27例,与擦拭法存在统计学差异(P0.05)。Chelex-100法、DNA IQTM法检出9个以上基因座分别为26例、24例,STR分型结果无统计学意义(P0.05)。提取牙刷的前、后部三束刷毛检出9个以上基因座分别达27例、28例,STR分型结果无统计学意义(P0.05);放置1天、1周、1个月、3个月、6个月的时间后检出9个以上基因座分别为28例、27例、22例、12例、7例。结论割取法提取一次性牙刷上的脱落细胞进行STR分型效果良好;放置时间越长的牙刷,检出率越低。
[Abstract]:Objective to study the DNA extraction and STR typing of exfoliated cells from disposable toothbrush. The effect of storage time on STR typing was compared. Results in the 30 cases with high concentration of DNAs, more than 9 loci were detected in 27 cases. There was a statistical difference between P0.05G. Chelex-100 DNA IQTM method and wiping method. More than 9 loci were detected in 26 cases and 24 cases, respectively. The results of STR typing were not statistically significant (P 0.05). Before and after the extraction of toothbrush, more than 9 loci were detected in 27 cases and 28 cases respectively. The results of STR typing were not statistically significant (P 0.05). After one day, one week, one month, three months and six months, more than 9 loci were detected in 28 cases, 27 cases, 22 cases and 12 cases, respectively. Conclusion extraction of exfoliated cells from disposable toothbrush is effective for STR typing. The longer the toothbrush is placed, the lower the detection rate is.
【作者单位】：南方医科大学法医学研究所;广州市刑事科学技术研究所;
【基金】：公安部应用创新计划项目(2005yycxGDST136) 广东省科技计划项目(2005B33701008)
【分类号】：D919
【正文快照】： 牙刷作为日常生活中最常见的物品之一,对牙刷上脱落细胞的DNA提取及STR分型在法医DNA检验具有重要的意义[1]。本文对一次性牙刷上脱落细胞的检验方法进行研究,探讨一次性牙刷用于法医物证检验的可行性及影响DNA提取和STR分型的因素。1材料与方法1.1检材酒店使用的一次性牙刷

【共引文献】