大鼠支气管哮喘模型建立及心肌细胞p53、caspase-8表达研究

发布时间：2018-02-07 16:47

本文关键词： p53 caspase-8 哮喘药物副作用　出处：《四川大学》2005年硕士论文　论文类型：学位论文

【摘要】：研究背景及目的: 支气管哮喘病理生理学研究发现支气管哮喘易于并发心肌损害。作者曾对12例支气管哮喘猝死病例进行病例形态学研究,发现支气管哮喘除肺部病变外,还并发心肌细胞萎缩、坏死和瘢痕形成。支气管哮喘患者心肌损害的机理尚不清楚。另外有学者报道支气管哮喘常用治疗药肾上腺素可能促进心肌细胞凋亡。本实验目的是通过哮喘模型的建立以及应用不同的药物治疗来分析心肌细胞损害的机理。方法: 健康SD雄性大鼠120只,随机分为正常对照组、实验对照组、不治疗组、肾上腺素治疗组、氨茶碱治疗组以及肾上腺素氨茶碱联合治疗组六个实验组,每组又分为四小组(2W、4W、6W、8W),每小组5只大鼠。于实验第2W、4W、6W、8W分别处死大鼠。应用免疫组化技术对凋亡促进因子p53、凋亡启动因子caspase-8表达进行研究,用IMAGE图像分析系统对阳性结果进行半定量测定。结果: (1)正常对照组及实验对照组SD雄性大鼠p53、caspase-8呈阴性表达,随实验时间的延长,表达强度无表化;(2)各实验组心肌细胞p53、caspase-8阳性表达。表达面积及面积灰度积在第2周较对照组高(p0.01),随着时间的延长阳性信号表达面积及面积灰度积逐渐上升,第8周,达高峰。(3)
[Abstract]:Research background and purpose:
Bronchial asthma pathophysiology of bronchial asthma with myocardial damage. The author has easy to sudden death cases in 12 cases of bronchial asthma were morphologically studied were found in bronchial asthma lung lesions, but also complicated with myocardial cell atrophy, necrosis and scar formation. A mechanism of myocardial damage in patients with bronchial asthma is unclear. In addition, some scholars have reported that bronchial the commonly used drugs for the treatment of asthma adrenaline may promote the apoptosis of myocardial cells. The purpose of this experiment is to establish the mechanism through the asthma model and drug treated by analysis of myocardial cell injury.
Method:
Healthy male SD 120 rats were randomly divided into normal control group, experimental control group, no treatment group, epinephrine treatment group, aminophylline treatment group and epinephrine aminophylline treatment group and six experimental groups, each group was divided into four groups (2W, 4W, 6W, 8W), 5 rats per group. In the experiment of 2W, 4W, 6W, 8W respectively. The rats were sacrificed by immunohistochemistry on the apoptosis promoting factor p53, apoptosis factor caspase-8 expression study system for semi quantitative positive results of IMAGE was determined by image analysis.
Result:
(1) normal control group SD male rats and experimental group p53, the expression of caspase-8 was negative, with the time of the experiment, the expression intensity of tableless; (2) the myocardial cells of the experimental group p53, Caspase-8 positive expression. The expression product of gray area and area is higher than the control group in second weeks (P0.01). With the extension of time the positive signal expression area and gray area area gradually increased, eighth weeks, and reached the peak (3).

【学位授予单位】：四川大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R562.25;D919

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