人体组织的过敏性休克实验研究

发布时间：2018-03-02 09:21

本文关键词： 肥大细胞过敏性休克类胰蛋白酶 IL-2Rα 嗜酸性粒细胞趋化因子(Eotaxin) 免疫组化原位杂交　出处：《吉林大学》2006年硕士论文　论文类型：学位论文

【摘要】：肥大细胞(Mast Cell, MC)、嗜酸性粒细胞与变态反应性疾病有着密切的关系,在其发病过程中发挥重要的作用。过敏性休克是临床常见的急症之一,由于缺乏确切的实验室诊断标准,是导致医疗纠纷的一个重要原因,在非法行医案件中也时有发生。因此,研究过敏性休克发生的机制,确立可靠、准确的诊断指标,找出简单易行的诊断方法,在法医病理诊断中尤为重要。本实验应用类胰蛋白酶(Tryptase)、白细胞介素2受体(IL-2R)、嗜酸性粒细胞趋化因子(Eotaxin)、肿瘤坏死因子α(TNF-α)免疫组化染色及检测Eotaxin基因阳性表达的原位杂交方法观察了30例过敏性休克致死和8例非过敏性休克致死病例的各脏器,结果如下: 通过以上研究,得到如下结论: 1.过敏性休克致死尸检病例中肺组织、肠、喉组织肥大细胞(MC)数目增多。 2.类胰蛋白酶可以作为MC特异性标记检测组织中MC的数量。 3.尸检病例肠及肺等组织中大量嗜酸性粒细胞的存在提示过敏性休克死亡。 4.TNF-α、IL-2Rα参与过敏性休克的病理过程。 5.过敏性休克时,肺、肾、肠、喉Eotaxin基因阳性表达染色阳性。Eotaxin免疫组化染色阳性。本实验应用免疫组化染色和原位杂交的方法,通过研究过敏性休克中MC的形态变化,证实在过敏性休克过程中类胰蛋白酶、TNF-α、IL-2Rα和Eotaxin发挥的作用,为其在法医病理实践中的应用提供了切实的理论依据。
[Abstract]:Mast cell, MCN, eosinophil are closely related to allergic diseases and play an important role in the pathogenesis. Anaphylactic shock is one of the common clinical emergencies, due to the lack of accurate laboratory diagnostic criteria. Is an important cause of medical disputes, and also occurs in illegal medical cases. Therefore, to study the mechanism of anaphylactic shock, to establish reliable and accurate diagnostic indicators, to find out a simple and easy diagnostic method, In this study, we used trypsin like trypsin, interleukin-2 receptor IL-2R, eosinophil chemokine eotaxin, tumor necrosis factor 伪 -TNF- 伪 to detect the expression of Eotaxin gene in situ. The viscera of 30 cases of anaphylactic shock and 8 cases of non-anaphylactic shock were observed by hybridization. The results are as follows:. Through the above research, the following conclusions are drawn:. 1. The number of mast cells in lung, intestine and larynx was increased in the autopsy patients with anaphylactic shock. 2. Trypsin can be used as a specific marker to detect the number of MC in tissues. 3. The presence of a large number of eosinophils in intestinal and lung tissues suggests anaphylactic shock death. 4. TNF- 伪 and IL-2R 伪 participate in the pathological process of anaphylactic shock. 5. In anaphylactic shock, Eotaxin gene expression was positive in lung, kidney, intestine and larynx. In this study, immunohistochemical staining and in situ hybridization were used to study the morphologic changes of MC in anaphylactic shock, and to confirm the role of TNF- 伪 IL-2R 伪 and Eotaxin in the process of anaphylactic shock. It provides a practical theoretical basis for its application in forensic pathology practice.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：D919

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