在线样品浓缩毛细管区带电泳分析毛发中的苯丙胺类毒品

发布时间：2018-04-05 04:07

本文选题：毛细管区带电泳　切入点：在线场放大样品堆积　出处：《分析化学》2006年08期

【摘要】：建立了毛细管区带电泳(CZE)的在线场放大样品堆积(FASS)方法。采用含有40%乙烯乙二醇的100 mmol/L磷酸盐二元缓冲液(pH 2.5),80%异丙醇的0.1 mmol/L磷酸样品溶液,利用缓冲体系与样品溶液体系电导率的差异,在毛细管中浓缩样品组分,对苯丙胺、甲基苯丙胺、亚甲基二氧基苯丙胺(MDA)、亚甲基二氧基甲基苯丙胺(MDMA)4种毒品进行了分离和定量测定,检测的灵敏度提高约1000倍。对于标准品的检出限可达到0.06μg/L。当样品浓度高于5μg/L时,分析的相对标准偏差在10%范围之内;用该方法对添加毒品的毛发进行了提取和测定,可检测到的添加浓度为1μg/g毛发。该方法可用于生物检材中苯丙胺类毒品的检测。
[Abstract]:An on-line FASS method for capillary zone electrophoresis (CZE) was developed.A #number0# mmol/L phosphate binary buffer solution containing 40% ethylene glycol, pH 2.5 and 80% isopropanol, was used to prepare the 0.1 mmol/L phosphoric acid sample solution. By using the difference of electrical conductivity between the buffer system and the sample solution system, the sample component was concentrated in the capillary.Four drugs, methamphetamine, methylene dioxy amphetamine (MDAA) and methylene dioxy methamphetamine (MDMA), were separated and quantitatively determined. The sensitivity of detection was increased by about 1000 times.The detection limit of the standard product is 0.06 渭 g / L.When the concentration of the sample is higher than 5 渭 g / L, the relative standard deviation of the analysis is within 10%, and the hair added with drugs is extracted and determined by this method. The detected concentration of the added hair is 1 渭 g / g hair.The method can be used for the detection of amphetamines in biological samples.
【作者单位】：中国人民公安大学刑事科学技术系
【基金】：公安部项目(No.20049123401) 北京教育委员会共建项目建设计划(No.SYS1004100436)资助
【分类号】：D919.2

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