低压电损伤大鼠脑组织c-jun表达及皮肤组织特殊染色的研究

发布时间：2018-04-11 13:35

本文选题：低电压 + 电流损伤　；参考：《山西医科大学》2007年硕士论文

【摘要】： 目的:(1)建立低电压大鼠电击伤模型,借以探讨大鼠电击死机理。(2)探讨生前电击与死后电击的皮肤的特殊染色是否有区别,能否用于生前与死后的鉴定。(3)检测生前电击与死后电击的C-JUN蛋白的表达,探讨能否用于生前电击与死后电击的鉴别。方法:(1)Wistar大鼠60只,雌雄不限,200±10g,随机分为3组:预实验组10只;实验组40只;对照组10只。摸索调整电击时间以及间隔时间,停止电击后即刻采用BL-420E+生物机能实验系统监测大鼠心电图变化。(2)Wistar大鼠18只,雌雄不限,200±10g,随机分为3组:生前电击死组6只;死后电击组6只;非电击对照组6只。利用(1)中设计的电击模型进行电击,取电击处及非电击处皮肤行苏木素-伊红(HE)染色、Mowory(ABPAS)法、Alcian blue-核固红法染色。(3)Wistar大鼠60只,雌雄不限,200±10g,随机分为2组,实验组及对照组。其中实验组又分为电击伤后1h,2h,4h,8h处死组,死后即刻电击组,死后15min,30min,1h;对照组分为生前实验对照组,死后实验对照组。电击伤组及死后组电击0.35s,间隔5s。取全脑,石蜡切片,免疫组化AEC染色。结果:(1)20例电击伤组大鼠电击前及10例正常对照组大鼠心电图均正常,P波向上,QRS波群呈Rs型,T波向上。20例电击伤组大鼠于电后即刻监测心电图,均可观察到S-T段有不同程度的抬高,其中有19例出现明显心动过速。(2)皮肤组织HE染色:生前电击组与对照组相比最明显的改变是真皮层胶原纤维肿胀、均质化,损伤中心基底层细胞核纵向伸长,染色较深;死后电击组改变类似于生前电击组。皮肤组织Mowory(ABPAS)法染色:死后电击组与生前电击组最大区别在于网织层胶原纤维及弹性纤维基本保持原有形态且蓝染,核深染,均质化不明显。正常对照组皮肤均未见异常表现。皮肤组织Alcian blue-核固红法染色:生前电击组真皮乳头层、网织层胶原纤维及弹性纤维均质化且明显较表皮层淡染,纤维组织的细胞核红染散在于网织层内。死后电击组网织层胶原纤维及弹性纤维基本保持原有形态,整个纤维均蓝染,而纤维细胞核红染。正常对照组皮肤均未见异常表现。(3)电击伤1h、2h、4h、各组大脑皮层及海马部位C-JUN蛋白呈强阳性表达,其中以4h阳性信号最强,电击伤后8h上述器官仅有微弱表达;死后电击组除死后即刻电击有微弱表达外,余均未检测到阳性表达;各实验对照组及死后电击各组脑组织基本检测不到C-JUN蛋白的表达。结论:(1)该电击伤模型动物具有明显电流损伤的特点,可用于电流损伤的法医学研究。(2)生前电击与死后电击的皮肤Alcian blue特殊染色有区别,可作为鉴别生前电击与死后电击的辅助指标。(3)生前电击、死后电击大鼠脑组织中C-JUN蛋白表达有区别,且其表达与电击伤后经过时间有一定相关性,可以作为鉴别生前电击与死后电击的一个指标。
[Abstract]:Objective: to establish a low-voltage rat model of electric shock injury in order to explore the mechanism of electrocution in rats. (2) to explore the difference between antemortem electric shock and postmortem shock on the skin.Whether it can be used in the identification of antemortem and postmortem shock, the expression of C-JUN protein in antemortem shock and postmortem shock was detected, and the identification of antemortem shock and postmortem shock was discussed.Methods 60 male and female Wistar rats were randomly divided into 3 groups: experimental group (n = 10), experimental group (n = 40) and control group (n = 10).The electrocardiogram (ECG) of 18 rats were monitored by BL-420E biological function test system immediately after the electric shock was stopped. The rats were randomly divided into 3 groups: antemortem electroshock group (n = 6), postmortem shock group (n = 6) and postmortem shock group (n = 6).There were 6 rats in the control group.The experimental group was divided into two groups: one hour, two hours and four hours and eight hours after the injury, and the control group was divided into two groups: the antemortem experimental group, the postmortem experimental control group, the postmortem experimental control group, the postmortem experimental control group and the postmortem experimental control group.The electric shock time was 0.35 s in the electrocution group and the postmortem group, the interval was 5 s.The whole brain was stained with paraffin section and immunohistochemical AEC staining.Results the electrocardiogram (ECG) of 20 rats in the control group and 10 normal rats in the control group were in the form of Rs / T wave. The electrocardiogram was monitored immediately after electric shock in the 20 cases of electric injury group (n = 20) and the normal control group (n = 10).It was observed that the S-T segment was elevated in varying degrees, including 19 cases with obvious tachycardia.) HE staining of skin tissue was observed. The most obvious changes in the antemortem shock group compared with the control group were the swelling and homogenization of collagen fibers in the dermis.The nuclei of the basal layer of the injured center were elongated longitudinally and stained deeply, and the changes of the postmortem shock group were similar to those of the antemortem shock group.No abnormal skin manifestations were found in the normal control group.After death, the reticulated collagen fibers and elastic fibers remained in their original form, the whole fibers were blue stained, and the nuclei of the fibers were red stained.In the normal control group, there was no abnormal appearance in the skin. No abnormal expression was found in the skin of the control group. The C-JUN protein was strongly expressed in the cortex and hippocampus of all groups at 1 h and 2 h and 4 h after the injury. The strongest positive signal was found in the cortex and hippocampus at 4 h, and the weak expression was observed in the above organs at 8 h after electrical injury.In the postmortem shock group, the expression of C-JUN protein was not detected except the weak expression in the immediate postmortem shock group, and the expression of C-JUN protein was not detected in the brain tissues of the experimental control group and the postmortem shock group.Conclusion (1) the model animal model of electric shock has obvious characteristics of electric current injury. It can be used in forensic research of electric current injury. (2) there is a difference between antemortem electric shock and postmortem electric shock in skin Alcian blue special staining.The expression of C-JUN protein in the brain tissue of postmortem shock rats was different, and the expression of C-JUN protein was correlated with the time elapsed after electric shock injury, which could be used as an auxiliary index for distinguishing antemortem electric shock from postmortem shock.It can be used as an indicator to distinguish antemortem shock from postmortem shock.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：D919

【引证文献】