固相萃

发布时间：2018-04-16 11:05

本文选题：巴比妥类 + 苯并二氮卓类　；参考：《沈阳药科大学》2002年硕士论文

【摘要】：常见的催眠安定剂有巴比妥类，苯并二氮卓类和吩噻嗪类药物。由于这些药物使用广泛，比较容易获得，，因此自杀、他杀、服用过量等原因引起的中毒、致死案件时有发生，是目前司法鉴定中常见的药物。对于该类药物的分析检测，目前主要采用液液萃取普通紫外光谱测定法，液液萃取分离法操作繁琐、费溶剂、药物萃取率低，杂质去除不彻底。固相萃取是70年代以来在液相色谱原理基础上发展起来的具有操作简便快速、萃取率高等优点的分离提取方法。这一技术避免了液液萃取的不足，堪称分离技术的一次革命。紫外导数光谱法是70年代以来在紫外光谱法基础上发展起来的具有操作简便快速、能有效消除杂质干扰、灵敏度较高等优点的检测方法。固相萃取、紫外导数光谱法国内研究尚少。本论文的研究目的是将两种方法相结合，建立起一种药物萃取率高、杂质去除彻底，可适合安眠药物中毒、致死案件检验、以操作简便快速为主要特点的分析方法。 1 巴比妥药物固相萃取——紫外差示导数光谱检测法本文建立了血、尿、肝、肾、肺、脑中巴比妥、苯巴比妥、戊巴比妥、异戊巴比妥、速可眠的分析方法。血、尿用水稀释，脏器用6％高氯酸沉淀蛋白后，加0.4mLGDX_(301)树脂振摇，滤集树脂于小层析柱中，用
[Abstract]:Common hypnotizers are barbiturates, benzodiazepines, and phenothiazines.Because these drugs are widely used and relatively easy to obtain, suicide, homicide, overdose and other causes of poisoning, death cases occur from time to time, is a common drug in forensic expertise.For the analysis and detection of this kind of drugs, the main methods are liquid-liquid extraction ordinary ultraviolet spectrophotometry, liquid-liquid extraction separation method is cumbersome, expensive solvent, drug extraction rate is low, impurity removal is not complete.Solid phase extraction (SPE) is a separation and extraction method developed on the basis of the principle of liquid chromatography since 1970s, which has the advantages of simple and rapid operation and high extraction efficiency.This technique avoids the shortage of liquid-liquid extraction and is a revolution in separation technology.Ultraviolet derivative Spectrometry (UV DDS) has been developed since 1970s on the basis of UV Spectroscopy. It has the advantages of simple and rapid operation, effective elimination of impurity interference, high sensitivity and so on.Solid phase extraction (SPE) and UV derivative spectroscopy are few in France.The purpose of this paper is to combine the two methods to establish an analytical method with high extraction rate, complete removal of impurity, suitable for poisoning of sleeping pills and lethal case testing, which is characterized by simple and rapid operation.Solid Phase Extraction-UV differential derivative Spectrometry for the determination of 1 BarbitoneA method for the analysis of barbitone, phenobarbital, pentobarbital, isoamobarbital and rapid sleep in blood, urine, liver, kidney, lung and brain was established.The blood and urine were diluted with water, the viscera were precipitated with 6% perchloric acid, and the resin was vibrated with 0.4mLGDX / 301) resin. The filter resin was used in the small chromatographic column.
【学位授予单位】：沈阳药科大学
【学位级别】：硕士
【学位授予年份】：2002
【分类号】：D919.2

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