兔悬挂双前肢致死组织中PKC、AchE、HSP70的活性变化

发布时间：2018-05-17 22:16

本文选题：法医病理学 + 限制性体位窒息　；参考：《中国法医学杂志》2010年01期

【摘要】：目的观察兔悬挂双前肢致死不同组织中PKC、AchE、HSP70的活性变化,探讨其在死亡机制中的作用。方法家兔双前肢悬挂致死亡,取膈肌、肋间肌、心肌、腓肠肌、大脑、脑干、肝、肾、肺等组织,以勒颈、颅脑损伤和疾病死亡相应组织为对照。采用SP法对上述组织中蛋白激酶C(PKC)、乙酰胆碱酯酶(AchE)和热休克蛋白70(HSP70)进行免疫组化染色,观察其活性,并采用多组独立样本的Kruskal-Wallis秩和检验方法,对结果进行统计学分析。结果膈肌、肋间肌和大脑组织细胞质中PKC免疫组化阳性染色,悬挂组较其它组增强(P0.05);膈肌、肋间肌、心肌、大脑和肝细胞质及肌组织神经束中AchE免疫组化阳性染色,悬挂组较其它组增强(P0.05);大脑、心、肺中HSP70免疫组化阳性染色,悬挂组较其它组增强(P0.05)。结论PKC、AChE、HSP70通过不同机制,在悬挂双上肢体位性窒息死亡中起到一定作用,本文结果可为相关研究及鉴定提供实验依据。
[Abstract]:Objective to observe the changes of PKC AchESP70 activity and its role in the death mechanism in different tissues of rabbits killed by suspension of double forelimbs. Methods the tissues of diaphragm, intercostal muscle, myocardium, gastrocnemius, brain, brain stem, liver, kidney and lung were taken as control group. The activity of protein kinase (PKC), acetylcholinesterase (ache) and heat shock protein 70 (HSP70) in these tissues were observed by SP method. The results were analyzed statistically by Kruskal-Wallis rank sum test of many groups of independent samples. Results PKC immunohistochemical staining was positive in the cytoplasm of diaphragm, intercostal muscle and cerebrum, and AchE immunohistochemical staining was observed in diaphragm, intercostal muscle, myocardium, cerebral and hepatic cytoplasm and muscle nerve tract in suspension group compared with other groups. Compared with the other groups, the suspension group enhanced P0.05G, and the brain, heart, lung, HSP70 immunohistochemical staining, the suspension group enhanced the P0.05P 0.05 compared with the other groups. Conclusion PKC AChEN HSP70 may play an important role in the death of asphyxia caused by suspension of upper limbs through different mechanisms. The results of this study can provide experimental evidence for related research and identification.
【作者单位】：中山大学基础医学院法医学系;广州市人民检察院技术处;广东省公安厅刑事技术中心;
【基金】：公安部科研项目(20034421201) 广东省科委基金项目(2003C33701)
【分类号】：D919.1

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