IL-1，IL-8，VEGF在小鼠皮肤切创中的表达及其与损伤时间关系的研究

发布时间：2018-05-19 10:28

本文选题：皮肤创伤 + 免疫组化　；参考：《吉林大学》2006年硕士论文

【摘要】：目的:探讨IL-1,IL-8及VEGF在小鼠皮肤切创中的表达及其与损伤时间的关系。方法:用免疫组化方法检测不同损伤时间(生前伤5min～9d;死后伤5min~30min)小鼠皮肤切创组织中IL-1,IL-8及VEGF的表达变化。结果:①IL-1, IL-8, VEGF除了在上皮细胞的表达外,还大量出现在肉芽组织的巨噬细胞和成纤维细胞内,而死后损伤组相对于正常对照组上述因子无异常表达。②皮肤切创后,在早期可诱导IL-1表达,5h达第一次高峰,1d下降。③伤后3h的损伤皮肤组织中可见部分的多核粒细胞表达IL-8,伤后12h～1d ,大部分浸润的多核粒细胞及部分单核细胞为IL-8阳性。随伤后时间延长, IL-8阳性细胞以单核及成纤维细胞为主。④VEGF在表皮及皮脂腺呈持续性阳性表达,持续至伤后12h和3d。结论:IL-1, IL-8, VEGF可作为推断皮肤损伤时间的法医病理学标志。
[Abstract]:Aim: to investigate the expression of IL-1 IL-8 and VEGF in mouse skin injury and the relationship between IL-8 and injury time. Methods: the expression of IL-1IL-8 and VEGF were detected by immunohistochemical method in the skin of mice with different injury time (5 min or 9 d before death and 30 min after death). Results in addition to the expression of IL-1, IL-8 and VEGF in epithelial cells, they also appeared in macrophages and fibroblasts of granulation tissue. However, there was no abnormal expression of 2. 2 in postmortem injury group compared with normal control group. At the early stage, the expression of IL-1 could be induced to reach the first peak at 1 day. 3. The expression of IL-8 in the injured skin tissue was decreased 3 h after injury. The expression of IL-8 was found in some of the multinucleated granulocytes, 12h~1d, most of the infiltrated multinucleated granulocytes and some monocytes were IL-8 positive. The expression of IL-8 in epidermis and sebaceous gland continued to be positive in the epidermis and sebaceous gland after injury for 12 hours and 3 days after injury, and the positive cells were mainly monocytes and fibroblasts. Conclusion: 1-IL-1, IL-8 and VEGF can be used as forensic pathological markers for estimating the time of skin injury.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：D919

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