利多卡因在硬膜外麻醉意外致死犬体内的死后再分布研究

发布时间：2018-05-31 03:20

本文选题：利多卡因 + 死后再分布　；参考：《山西医科大学》2005年硕士论文

【摘要】：目的 (1)建立犬的利多卡因静脉注射、蛛网膜下腔麻醉、硬膜外麻醉致死的死后再分布动物模型; (2)比较三种死后再分布模型致死犬常温下不同时间点利多卡因在心血、周围血、肝脏和脑中的分布规律,为利多卡因硬膜外麻醉意外死亡和中毒案件的法医学鉴定提供科学依据; (3)比较三种死后再分布模型致死犬在常温下不同时间点心血、周围血、肝脏和脑中利多卡因的分布特点, 探讨死后再分布机制。方法 1 动物模型每种模型各用犬9 只,随机分为实验组(6 只)和对照组(3 只)两组,实验组犬5 分钟内分别经股静脉、蛛网膜下腔、硬膜外腔匀速注入利多卡因5×15mg/kg,对照组分别在对应部位注入等体积生理盐水。 2 生命体征记录方法BL-生物机能实验系统全程记录死后再分布模型从开始给药到动物死亡的心电、血压和呼吸等主要生命体征的变化。 3 样品采集与处理心电、血压和呼吸全部消失后,置于室温下,分别于死后0,1,4,8,24,48,72 小时取心血、周围血、肝脏、大脑,检测实验组犬上述检材中利多卡因含量,对照组犬各对应检材用作空白; 样品经酸、碱化处理后,乙醚萃取,根据利多卡因的特征离子峰、保留时间气相色谱-质谱联用定性,内标法、工作曲线法气相色谱定量。结果 ( 1 ) 症状静脉注射实验组动物开始给药后出现瞳孔缩小、呼吸困难、口吐粘稠状白色泡沫、角弓反张、大小便失禁。蛛网膜下腔麻醉、硬膜外腔麻醉实验组动物出现症状与上述相似。三种模型对照组均未出现以上症状,未出现死亡。
[Abstract]:Purpose 1) to establish the animal model of lidocaine intravenous injection, subarachnoid anesthesia and epidural anesthesia. (2) to compare the distribution of lidocaine in heart blood, peripheral blood, liver and brain at different time points at room temperature in three kinds of postmortem redistributive models. To provide scientific basis for forensic identification of lidocaine epidural anesthesia accidental death and poisoning cases. (3) to compare the distribution characteristics of lidocaine in heart blood, peripheral blood, liver and brain of three kinds of postmortem redistributive model dogs at different time points under normal temperature, and to explore the mechanism of postmortem redistribution. Method 1Nine dogs with each animal model were randomly divided into two groups: the experimental group (n = 6) and the control group (n = 3). The dogs in the experimental group were treated by femoral vein and subarachnoid cavity within 5 minutes. Lidocaine was injected into the epidural cavity at a uniform rate of 5 脳 15 mg / kg, and the control group was injected with saline of the same volume in the corresponding parts. 2 vital signs recording method BL-Biological function Experimental system recorded the changes of the main vital signs from the beginning of administration to the death of animals, blood pressure and respiration in the whole course of the model of postmortem redistribution. 3 sampling and processing After the disappearance of ECG, blood pressure and respiration, they were placed at room temperature. The blood samples, peripheral blood, liver and brain were collected for 72 hours after death. The lidocaine content in the above samples was detected in the experimental group, and the corresponding samples in the control group were used as blank. The samples were extracted by ether after acid and alkali treatment. The retention time was determined by gas chromatography-mass spectrometry (GC-MS) according to the characteristic ion peak of lidocaine. Result The main results were as follows: (1) the symptoms of the experimental group were pupillary dilatation, dyspnea, sticky white foam, angle arch reversion, incontinence. The symptoms of subarachnoid anesthesia and epidural anesthesia in experimental group were similar to those mentioned above. The above symptoms and death were not found in the three model control groups.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：D919

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