过敏性猝死人体内P物质表达的免疫组化研究

发布时间：2018-06-24 02:57

本文选题：P + 物质　；参考：《山西医科大学》2005年硕士论文

【摘要】：目的观察P 物质在过敏性猝死人体肺脏、咽喉和胃肠组织的表达,应用组合染色方法显示肥大细胞脱颗粒现象,并探讨其在法医学鉴定中的作用和意义,试图为过敏性猝死的法医学鉴定提供可靠的形态学依据。方法(1)应用免疫组化方法对15 例过敏性猝死者肺脏、咽喉和胃肠组织的P 物质表达进行研究,同时以20 例冠心病猝死者作为对照组;应用BI-2000 计算机图像分析系统对P 物质免疫组化染色结果进行分析,计算阳性指数(PI)。(2)应用俾士麦棕-碘绿-马汀黄(BB-IG-MY)组合染色法显示肥大细胞脱颗粒现象。结果(1)与对照组相比,实验组咽喉和胃肠组织P 物质表达明显增加。统计学t 检验结果为:咽喉粘膜组t=6.942,咽喉腺体组t=4.601,胃肠组t=5.025,全部p 值小于0.001,均有统计学意义。(2)实验组和对照组肺组织细支气管壁周围未见P 物质阳性染色。(3)组合染色法结果表明,肥大细胞胞质颗粒呈深棕色,细胞核呈绿色,红细胞呈黄色;对照组肥大细胞未见脱颗粒现象,而实验组肥大细胞脱颗粒现象明显。结论过敏性猝死时,咽喉、胃肠组织的P 物质表达明显增加,BB-IG-MY 组合染色法可清晰显示过敏时肥大细胞脱颗粒现象。这些指标可作为过敏性猝死的法医学鉴定的形态学依据和参考指标。
[Abstract]:Objective to observe the expression of substance P in the lung, throat and gastrointestinal tissues of patients with sudden allergic death, and to demonstrate the degranulation of mast cells by combined staining, and to explore the role and significance of substance P in forensic identification. This paper attempts to provide a reliable morphological basis for forensic identification of sudden allergic death. Methods (1) Immunohistochemical method was used to study the expression of substance P in lung, throat and gastrointestinal tissues of 15 patients with sudden allergic death and 20 patients with sudden death of coronary heart disease as control group. The immunohistochemical staining results of substance P were analyzed by BI-2000 computer image analysis system. The positive index (Pi). (2) was calculated and the mast cell degranulation was demonstrated by the combination staining method of Pygmy brown iodide green marting yellow (BB-IG-MY). Results (1) compared with the control group, the expression of substance P in the throat and gastrointestinal tissue of the experimental group was significantly increased. The results of t test were as follows: t of throat mucosa group was 6.942, of throat gland group was 4.601, of gastrointestinal group was 5.025, all p values were less than 0.001. (2) there was no positive staining of substance P around bronchiolus wall in experimental group and control group. (3) combination group. The results of staining showed that, The cytoplasmic granules of mast cells were dark brown, the nuclei were green and the red blood cells were yellow. In the control group, the degranulation of mast cells was not seen, but the degranulation of mast cells in the experimental group was obvious. Conclusion during sudden anaphylactic death, the expression of substance P in larynx and gastrointestinal tissues increases significantly by BB-IG-MY combined staining method, which can clearly show the degranulation of mast cells in allergy. These indexes can be used as morphological basis and reference index for forensic identification of sudden allergic death.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：D919

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