大鼠脑组织缺血GFAP、HSP70的表达与缺血时间推断的研究

发布时间：2018-07-01 15:06

本文选题：脑缺血 + 胶质原纤维酸性蛋白　；参考：《山西医科大学》2005年硕士论文

【摘要】：目的探讨大鼠脑组织缺血后不同时间胶质原纤维酸性蛋白(Glial fibrillary acidic protein,GFAP)和热休克蛋白70(Heat shock protein 70,HSP70)的表达,旨为法医实践中推断脑组织缺血时间提供科学的参考依据。方法建立Wistar 大鼠脑组织缺血模型,应用免疫组化S-P 法与显微图像分析技术检测脑组织缺血后10min、30min、1h、3h、6h、12h、24h、48h 和正常组GFAP、HSP70 的表达,观察是否有统计学意义。结果(1)大鼠海马区神经细胞缺血3h 可见水肿、坏死,锥体细胞受损;缺血48h 见大量锥体细胞坏死,核膜崩解、核仁消失而仅留细胞碎屑。(2)脑组织缺血3h,GFAP 在星形胶质细胞的阳性表达与正常组比较无差异; 6h 与3h 比较有差异(p 0.05);12-48h 各组间比较有显著性差异(p 0.01)。(3)HSP70 的表达随着缺血时间的延长而逐渐增高,HSP70 阳性细胞于脑缺血后10min 后开始出现,随着缺血时间的延长开始逐渐增多,缺血6h 内各组间比较无差异;6-12h 各组间比较有差异(p 0.05);24-48h各组间比较有显著性差异(p 0.01)。结论大鼠脑组织缺血GFAP、HSP70 阳性表达呈现一定规律,与缺血时间明显相关,可作为推断脑缺血时间的参考指标之一,在法医检案中具有应用价值。
[Abstract]:Objective to investigate the expression of Glial fibrillary acidic protein (GFAP) and heat shock protein 70 (Heat shock protein 70, HSP70) at different time after cerebral ischemia in rats, and to provide a scientific reference for the inference of cerebral ischemic time in forensic practice.
Methods the cerebral ischemia model of Wistar rats was established, and the immunohistochemical S-P method and microscopic image analysis technique were used to detect 10min, 30min, 1H, 3h, 6h, 12h, 24h, 48h and GFAP, HSP70, and whether there was statistical significance.
Results (1) edema, necrosis, and pyramidal cells were damaged in the ischemic 3H in the hippocampus of the rats, and the ischemic 48h saw a large number of pyramidal cells necrosis, the nuclear membrane disintegrating and the nucleolus disappeared and only the cell debris left. (2) the cerebral ischemia 3h, the positive expression of GFAP in astrocytes was not different from that in the normal group; 6h and 3H were different (P 0.05); 12-48h There were significant differences in each group (P 0.01). (3) the expression of HSP70 increased gradually with the prolongation of ischemic time. The HSP70 positive cells began to appear after 10min after cerebral ischemia, and gradually increased with the prolongation of ischemic time. There was no difference between each group in the ischemic 6h (P 0.05), and the ratio of 24-48h to each group was compared. There was a significant difference (P 0.01).
Conclusion the positive expression of GFAP and HSP70 in the cerebral ischemia of rats is related to the ischemic time. It can be used as one of the reference indexes to infer the time of cerebral ischemia, and it is valuable in forensic examination.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：D919

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