白细胞介素-8及受体基因多态性与银屑病的相关性研究

发布时间：2018-07-02 14:49

本文选题：银屑病 + 白细胞介素-8　；参考：《四川大学》2007年硕士论文

【摘要】： 背景：银屑病(psoriasis，PS)是一种自身免疫系统慢性调节障碍导致的炎性皮肤过度增生性疾病，在人群中的发病率约0．1-3％。我国1984年进行的全国银屑病流行病学调查认为总的患病率为0．123％，据此估计目前我国银屑病患者超过300万。严重的银屑病不仅使患者生活质量下降，而且医药费用高昂，美国每年在该病医疗花费高达16亿美元，尽管如此，目前依然没有有效的治疗方法。引起银屑病的病因与发病机制目前尚不清楚。近年来的流行病学研究高度提示人们，遗传因素在其病因学中具有重要地位。目的：基于我国银屑病的遗传研究现状和人类基因组数据库中IL-8和IL-8R相关资料，通过比较健康群体与银屑病群体在IL-8(-251)、IL-8R(Ser276Thr)、IL-8R(Met31Arg)、IL-8R(Arg335Cys)四个基因座上的基因型和等位基因频率，研究其与银屑病的关系，发现银屑病致病基因或者遗传易感基因，为银屑病的基因筛查、诊断、治疗等提供理论依据和技术支持。方法：(1)根据基因组数据库资料设计并合成特异性引物，确定酶切基因座。(2)随机选取中国汉族PS患者105例以及体检健康者105例作对照，，抽取外周静脉血，提取DNA。(3)对所提取的基因组DNA进行聚合酶链反应-限制性片段长度多态性(PCR-RFLP)的方法检测。(4)基因型和等位基因频率采用基因直接计数法计算，各组间基因型及等位基因频率比较采用SPSS13．0软件的卡方检验(χ~)进行分析。结果：IL-8R(Met31Arg)的GT基因型在PS组出现的例数高于对照组，经校正性χ~2检验P=0．003，小于0．05，说明组间构成有明显差异；G等位基因在PS组出现的频率高于对照组，P=0．032，有统计学意义。IL-8R(Arg335Cys)的TT基因型频率高于对照组，P=0．003，小于0．05，统计学有显著性意义。IL-8(-251)、IL-8R(Ser276Thr)的基因型和等位基因频率比较无统计学意义。结论：(1)IL-8R(Met31Arg)基因座可能与银屑病易感性正相关，会加大银屑病的遗传易感性，可作为银屑病易感性的一个判断指标。 (2)IL-8R(Arg335Cys)基因座TT基因型可能调控IL-8R的表达和功能，从而银屑病疾病的发生和病程相关联。 (3)在所研究人群中未发现IL-8(-251)、IL-8R(Ser276Thr)基因座基因型和等位基因与银屑病遗传易感性相关。
[Abstract]:Background: psoriasis (psoriasis) is an inflammatory skin hyperproliferative disease caused by chronic regulatory disorders of the autoimmune system. The incidence of psoriasis in the population is about 0.1-3. The prevalence rate of psoriasis was estimated to be more than 3 million in China, according to the national epidemiological survey of psoriasis in 1984. Severe psoriasis not only reduces patients' quality of life, but also costs a lot of medicine. The United States spends as much as $1.6 billion a year on the disease, but there are still no effective treatments. The etiology and pathogenesis of psoriasis are unclear. Epidemiological studies in recent years highly suggest that genetic factors play an important role in its etiology. Objective: to compare the genotypes and alleles frequencies of IL-8 and IL-8R on IL-8 (-251) IL-8R (Ser276Thr) / IL-8R (Met31Arg) IL-8R (Arg335Cys) loci between healthy population and psoriatic population based on the status quo of psoriasis genetics in China and the relevant data of IL-8 and IL-8R in human genome database. By studying the relationship between psoriasis and psoriasis, we found that psoriasis pathogenicity genes or genetic susceptibility genes provide theoretical basis and technical support for gene screening, diagnosis and treatment of psoriasis. Methods: (1) specific primers were designed and synthesized according to genomic database data. (2) 105 Chinese Han patients with PS and 105 healthy controls were randomly selected to extract peripheral venous blood. (3) Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect the extracted genomic DNA. (4) genotypes and alleles were calculated by direct gene counting. Genotypes and allelic frequencies were analyzed by Chi-square test (蠂 ~) using SPSS 13.0 software. Results the number of GT genotypes in PS group was higher than that in the control group. The corrected 蠂 ~ 2 test showed that there were significant differences in the composition of GT genotype between PS group and control group (P < 0.05, P < 0.003). The frequency of G allele in PS group was higher than that in control group (P < 0.032), and the frequency of TT genotype of IL-8R (Arg335Cys) was higher than that of control group (P < 0.05). There was significant difference between the genotype and allele frequency of IL-8 (-251) IL-8R (Ser276Thr). There was no statistical significance. Conclusion: (1) IL-8R (Met31Arg) locus may be positively correlated with the susceptibility to psoriasis and may increase the genetic susceptibility of psoriasis. (2) the TT genotype of IL-8R (Arg335Cys) may regulate the expression and function of IL-8R. Therefore, the occurrence of psoriasis was associated with the course of psoriasis. (3) there was no correlation between the genotype and allele of IL-8 (-251) IL-8R (Ser276Thr) locus and the genetic susceptibility of psoriasis.
【学位授予单位】：四川大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：D919

【引证文献】