缺氧预处理对大鼠钝性心肌挫伤保护作用的研究

发布时间：2018-07-05 10:13

本文选题：缺氧预处理 + 心脏挫伤　；参考：《山西医科大学》2006年硕士论文

【摘要】：目的：探讨缺氧预处理对大鼠钝性心脏挫伤的保护作用及其机制。方法：建立大鼠钝性心脏挫伤模型，健康成年wistar大鼠60只，随机分为缺氧预处理组、单纯挫伤组(每组各24只)和正常对照组(12只)，并将每组大鼠分为2h、4h、6h、12h四个时间段。挫伤前以O_2(10％)和N_2(90％)_2的混合气体进行全身缺氧3h，，常氧正常饲养24h复制缺氧预处理模型。HE染色观察大鼠心肌组织学改变，免疫组化法检测心肌组织中cTnT及bcl-2蛋白含量并用图像分析仪对其进行定量分析。结果：免疫组织化学染色(1)心脏挫伤2h可见大鼠心肌cTnT明显脱失，随着时间延长挫伤组cTnT脱失逐渐加重，与单纯挫伤组相比缺氧预处理组cTnT的脱失显著减轻(p＜0.05)，对照组心肌未见明显脱失；(2)对照组有少量bcl-2表达，挫伤2h组bcl-2表达减少，随时间延长挫伤组bcl-2表达逐渐增多，与假手术组和挫伤组相比HPC组bcl-2表达明显增强(p＜0.05)。结论：bcl-2的表达说明其参与了心脏挫伤后心肌损伤的形成。缺氧预处理对大鼠钝性心肌挫伤有保护作用，表现为HPC能减轻心肌细胞cTnT的脱失及促进bcl-2的表达。缺氧预处理的心肌保护作用很可能是通过上调bcl-2的表达来实现的，但其具体机制有待进一步研究。
[Abstract]:Objective: to investigate the protective effect and mechanism of anoxic preconditioning on blunt cardiac contusion in rats. Methods: a blunt cardiac contusion model was established in rats. 60 healthy adult wistar rats were randomly divided into hypoxia preconditioning group, simple contusion group (24 rats in each group) and normal control group (12 rats). Before contusion, the mixture of O _ 2 (10%) and N _ 2 (90%) was used for 3 h of systemic hypoxia. The hypoxic preconditioning model was induced by normoxic feeding for 24 h. The histological changes of myocardium were observed by HE staining. The contents of cTnT and bcl-2 protein in myocardial tissue were detected by immunohistochemical method and quantitatively analyzed by image analyzer. Results: immunohistochemical staining (1) the myocardial cTnT loss was observed at 2h after cardiac contusion in rats, and increased gradually with time prolonging in the contusion group. Compared with the simple contusion group, the loss of cTnT in hypoxia preconditioning group was significantly reduced (p < 0. 05), but no significant loss of cTnT was found in the control group. (2) there was a small amount of bcl-2 expression in the control group, while the bcl-2 expression decreased in the contusion group for 2 hours, and the bcl-2 expression in the contusion group gradually increased with the extension of time. The expression of bcl-2 in HPC group was significantly higher than that in sham operation group and contusion group (p < 0. 05). Conclusion the expression of BCL 2 suggests that it is involved in the formation of myocardial injury after cardiac contusion. Hypoxia preconditioning has protective effect on blunt myocardial contusion in rats, which shows that HPC can attenuate the loss of cTnT and promote the expression of bcl-2 in cardiac myocytes. The myocardial protective effect of hypoxia preconditioning may be achieved by upregulating the expression of bcl-2, but its mechanism needs further study.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：D919

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