IL-18与纤维组织在病变血管及心肌的表达及法医学意义

发布时间：2018-07-07 15:05

本文选题：病变血管 + IL-18　；参考：《重庆医科大学》2005年硕士论文

【摘要】：目的:实验拟从形态学研究动脉粥样硬化(AS)及 AS 致动脉破裂出血血管壁纤维成分的变化以及 IL-18 在上述血管和心肌的表达,探讨纤维组织和IL-18在动脉粥样硬化和/或高血压发病机制中的作用以及法医学意义。方法:1、用 Masson 三色染色法、渡边改良法、HE 染色等实验方法,对 A 组(AS 致动脉破裂组)10 例、B 组(AS 无破裂组)10 例、C组(正常对照组)15 例和 3 例 D 组(Ⅰ期原发性高血压组)标本进行特殊染色,分析三种纤维的成分,探讨纤维成分的变化及意义。 2、用免疫组化的方法,检测 IL-18 在上述标本的表达;同时检测IL-18 在 10 例心肌梗塞、11 例早期缺血和 13 例正常心肌的表达。探讨IL-18在动脉粥样硬化和/或原发性高血压发病机制中的作用及法医学意义。结果:1、主动脉壁三种纤维成分的分布与含量胶原纤维(CF)、弹力纤维(EF)、网状纤维(RF)在 C 组动脉壁各层排列较规则,未见断裂,其含量分别为 21.57±13.17%,32.39±4.14%,9.68±0.9%。 CF 在 A、B 组动脉壁各层明显增多,排列不规则,含量较 C 组增多4-5 倍,CF 含量在 A、B、C 组之间两两比较均有显著性差异(P0.01)。重庆医科大学硕士研究生学位论文 4 EF 断裂在 A、B 组动脉壁各层明显增多,含量较 C 组明显减少(50% 左右), A、B 组 EF 含量与 C 组比较有显著性差异(P0.01),A、B 组之间没有显著性差异(P0.05)。 A、B 组内膜下有较多散在 RF,SMCs 层 RF 排列不规则、缺失或减少、断裂、塌陷成团, A、B、C 组之间 RF 含量没有显著性差异(P0.05) 。在主动脉粥样斑块坏死区周围,CF 增多,排列不规则,EF 明显减少或缺失,无 RF;A 组坏死区 CF 很少甚至缺失;B 组坏死区 CF 增多, 并向弹力层伸入。 D 组除内膜 CF 稍增多外,其余各层 CF、EF、RF 各层较少,排列尚规则;EF 断裂不明显;RF 呈线条状。 2、各组冠状动脉壁 CF、EF、RF 含量及分布与主动脉类似。 3、 A、B 组脾中央动脉 CF 排列不规则,向 SMCs 浸润生长,含量较 C 组明显增多, A、B 组含量与 C 组有显著性差异(P0.01),A、B 组之间没有显著性差异(P0.05)。C 组 CF 分布在外膜、量少;D 组 CF 较 C 组增多;RF 分布于整个血管壁,排列较规则;含量在 A、B、C 组没有显著性差异(P0.05)。(注:Masson 染色难以区分脾中央动脉管壁 EF,故未进行统计分析)。 4、 IL-18 的表达 IL-18 在 A 组主动脉、冠状动脉、脾中央动脉血管内皮细胞(ECs)、内膜、炎性细胞(尤以巨噬细胞)及粥样斑块坏死区及周围表达强阳性; B 组呈阳性;C 组呈阴性;A、B、C 组之间两两比较,均有显著性差异(P0.05);D 组 IL-18 在主动脉、冠状动脉呈阳性,脾中央动脉 ECs 及炎性细胞呈弱阳性。 IL-18 在正常心肌(NM)呈阴性;早期心肌缺血(EMI)呈阳性; 心肌梗塞(MI)呈强阳性,NM、EMI 与 MI 之间比较均有显著性差异 (P0.05)。
[Abstract]:Objective : To investigate the changes of vascular wall fiber composition and the expression of IL - 18 in the vascular and myocardium of AS and AS , and to investigate the role of fibrous tissue and IL - 18 in the pathogenesis of atherosclerosis and / or hypertension , as well as the significance of forensic medicine .

Methods : The expression of IL - 18 in 10 cases , group B ( AS non - rupture group ) , group C ( normal control group ) in 10 cases , group B ( AS non - rupture group ) in 10 cases , group C ( normal control group ) in 10 cases , group B ( AS without rupture group ) , 15 cases in group C ( normal control group ) and 3 cases of D group ( stage I essential hypertension group ) were examined by immunohistochemistry .

Results : 1 . Distribution and content of three kinds of fiber components in aortic wall

Collagen fiber ( CF ) , elastic fiber ( EF ) , reticular fiber ( RF ) were regularly arranged in each layer of C - group arterial wall , and no fractures were observed . The contents were 21.57 卤 13.17 % , 32.39 卤 4.14 % and 9.68 卤 0.9 % , respectively .

In group A and group B , CF increased 4 - 5 times higher in group A and group B than in group C , and CF content was significantly different between group A , B and C ( P0.01 ) .

Master ' s Degree Thesis in Chongqing Medical University

4 .

EF broke obviously in the arterial wall of group A and B , and the content of EF was significantly decreased in group C ( 50 % ) .

The EF content in group A and B was significantly different from that in group C ( P0.01 ) .

There was no significant difference between them ( P0.05 ) .

In group A and group B , there were more scattered RF , irregular , missing or reduced RF arrangements in the intima .

There was no significant difference in RF content between groups A , B and C ( P0.05 ) .

There was an increase in CF , irregular arrangement , and a significant decrease in EF around the necrotic area of the aorta

less or absent , no RF ; little or even deletion of the necrosis area CF in group A ; increased CF in group B necrosis ,

and extends into the elastic layer .

In group D , the CF , EF and RF were less and the number of CF , EF and RF was less .

Rule ; EF breaks are not obvious ; RF is linear .

2 . The levels and distribution of CF , EF , RF in each group were similar to that of the aorta .

In group A and group B , the CF of central splenic artery was irregular , infiltration and growth were observed .

There was significant difference in group A , group B and group C ( P0.01 ) , A , B were higher than those in group C ( P0.01 ) .

There was no significant difference between the groups ( P0.05 ) . CF in group C was distributed in the outer membrane , the quantity was small , and the CF in group D was less than that of group D .

Increased number of C ; RF distribution throughout the vessel wall , arrangement of rules ; content in Groups A , B , C

There was no significant difference ( P0.05 ) .

Wall EF , no statistical analysis was performed ) .

4 . Expression of IL - 18

IL - 18 was in the aorta , coronary artery and central splenic artery endothelial cells ( ECs ) in group A ,

Intima , inflammatory cell ( especially macrophages ) and atherosclerotic plaques necrotic area and surrounding expression of strong yang

sex ; group B was positive ; group C was negative ; there were significant differences between group A , B and C .

Difference ( P0.05 ) ; Group D IL - 18 was positive in aorta , coronary artery and central splenic artery .

ECs and inflammatory cells were weakly positive .

IL - 18 was negative in normal myocardium ( NM ) ; early myocardial ischemia ( EMI ) was positive ;

Myocardial infarction ( MI ) was strongly positive , and there was a significant difference between NM , EMI and MI .

(P0.05).
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：D919

【参考文献】