两种邻苯二甲酸酯类污染物对斑马鱼胚胎发育的影响

发布时间：2017-12-27 19:09

本文关键词：两种邻苯二甲酸酯类污染物对斑马鱼胚胎发育的影响　出处：《中国环境科学》2017年09期 　论文类型：期刊论文

【摘要】：研究了邻苯二甲酸二辛酯(DEHP)和邻苯二甲酸二丁酯(DBP)对斑马鱼胚胎发育形态学指标的影响,并利用酶联免疫法检测了DEHP和DBP暴露后两种与胚胎发育相关的蛋白(Nkx2.5和LOX)及甲状腺素(T3、T4)水平的变化.结果发现试验浓度下(10~500μg/L)的DEHP和DBP可诱导斑马鱼胚胎出现一系列发育异常,包括自主运动异常、心率下降、脊柱弯曲及心包水肿等,同时伴随心脏发育蛋白Nkx2.5含量显著下降.ELISA检测结果表明,500μg/L的DEHP和DBP暴露后,斑马鱼胚胎T3、T4含量显著上升.上述结果表明DEHP和DBP对鱼类早期生命阶段同样具备内分泌干扰作用.另外,本研究中DEHP和DBP对斑马鱼胚胎的最低可观察效应浓度(LOEC)为10μg/L,已经接近它们在一些环境水域的检出浓度,因此其对水环境中处于早期生命阶段的生物的潜在危害急需重视.
[Abstract]:Study on the adjacent benzene two formic acid two ester (DEHP) and dibutyl phthalate (DBP) on morphological index of zebrafish embryos, and the detection of DEHP and DBP after exposure to two and embryonic development related protein by enzyme linked immunosorbent assay (Nkx2.5 and LOX) and thyroxine (T3, T4) were measured. Results showed that test concentrations (10~500 g/L) DEHP and DBP can induce a series of zebrafish embryo developmental abnormalities, including independent motion abnormalities, decreased heart rate, spinal curvature and pericardial edema, accompanied by the development of heart Nkx2.5 protein content decreased significantly.ELISA results showed that 500 g/L of DEHP and DBP increased significantly after exposure, zebrafish embryonic T3, T4 content. The results showed that DEHP and DBP also have endocrine disrupting effects on fish early life stages. In addition, DEHP and DBP in the study of zebrafish embryos the lowest observable effect The concentration (LOEC) is 10 g/L, which is close to their concentration in some environmental waters. Therefore, it is urgent to pay attention to the potential hazards of organisms in the early stage of life in water environment.
【作者单位】：中国水产科学研究院资源与环境研究中心;中国农业大学理学院;
【基金】：中央级公益性科研院所基本科研业务费专项(2016C008);中央级公益性科研院所基本科研业务费专项(2017HY-ZD0201)
【分类号】：X592
【正文快照】： 增塑剂(又称塑化剂)是一种能够增加材料可塑性、柔韧性和膨胀性的添加剂,在塑料生产中的使用十分频繁.邻苯二甲酸酯(PhthalateEsters,PAEs)是目前应用最为普遍的增塑剂,广泛用于食品包装、儿童玩具、建筑材料、医疗用品和化妆品等领域[1].邻苯二甲酸酯与塑料分子之间由氢键或

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