PM2.5对大鼠肺泡巨噬细胞的损伤及对HO-1的影响

发布时间：2018-07-10 11:08

本文选题：PM2.5 + 大鼠　；参考：《河北医科大学》2017年硕士论文

【摘要】：目的:了解不同浓度的PM2.5对大鼠肺泡巨噬细胞的损伤及对血红素氧合酶-1水平的影响。方法:以河北省石家庄市冬季雾霾天气采集的大气细颗粒物为实验材料,制作成不同浓度的PM2.5悬液(50、100、200、400μg/mL),以PBS缓冲液作为阴性对照组,分别染毒从健康雄性的Wistar大鼠肺泡灌洗液中提取、分离的肺泡巨噬细胞(alveolar macrophages,AM),染毒8小时后,采用MTT法测定肺泡巨噬细胞的存活率,用ELISA法检测HO-1的水平。数据分析:采用SPSS 21.0统计软件对所有数据进行统计分析。检测数据正态性及正态方差齐的计量资料采用单因素方差分析(One-Way ANOVA),进一步两两比较均采用使用SNK法;以P0.05为具有统计学意义。结果:随着PM2.5悬液浓度的增加,大鼠肺泡巨噬细胞的存活率逐渐下降,但是50μg/mL组与阴性对照组没有显著性差异,而100、200、400μg/mL组与阴性对照组及50μg/mL组相比则出现显著差异(P0.05);HO-1水平随着PM2.5悬液的浓度升高而逐渐升高,各组之间有统计学差异(P0.05)。结论:雾霾天气越严重,PM2.5对肺泡巨噬细胞的损伤越大,HO-1的水平随着PM2.5浓度的升高而增加,体现出其在雾霾刺激中起到的保护作用。
[Abstract]:Aim: to investigate the effects of different concentrations of PM2.5 on alveolar macrophage injury and heme oxygenase-1 level in rats. Methods: PM2.5 suspensions (50100,200400 渭 g / mL) were prepared from atmospheric fine particles collected in winter of Shijiazhuang City, Hebei Province. PM2.5 suspensions (50100,200400 渭 g / mL) were used as negative control group. Alveolar macrophages were isolated from the alveolar lavage fluid of healthy male rats respectively. The survival rate of alveolar macrophages was measured by MTT assay and the level of HO-1 was detected by Elisa after 8 hours of exposure. Data analysis: all data were analyzed by SPSS 21.0 software. One-Way ANOVA (One-Way ANOVA) was used to measure the normality and homogeneity of the measured data, and SNK was used for further comparison. Results: with the increase of PM2.5 suspension concentration, the survival rate of alveolar macrophages decreased gradually, but there was no significant difference between 50 渭 g / mL group and negative control group. There was significant difference between the 100200400 渭 g / mL group and the negative control group and 50 渭 g / mL group (P0.05). Conclusion: the more severe haze weather is, the greater the damage of PM2.5 to alveolar macrophages is, and the level of HO-1 increases with the increase of PM2.5 concentration, which shows the protective effect of PM2.5 on haze stimulation.
【学位授予单位】：河北医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：X513;R563

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