构巢曲霉锰过氧化物酶转化子菌株对6种染料的脱色

发布时间：2018-10-09 18:12

【摘要】：为了获得构巢曲霉(Aspergillus nidulans)锰过氧化物酶转化子菌株TN02A7-Lg-mnp2对不同染料的脱色效果。用分光光度法对酶活性检测的丙二酸钠缓冲溶液的pH进行了优化筛选,用单因素和正交试验对产酶条件进行了优化;检测了优化后的酶液对达旦黄、孔雀石绿、中性红、刚果红、曲利本蓝和结晶紫的脱色效果。菌株酶液对偶氮类的达旦黄、三苯基甲烷类的孔雀绿和杂环类的中性红都有显著的脱色效果,在1 h时脱色率分别为达94.41%、91.43%和90.00%,在16 h时脱色率分别为98.36%、100%和96.47%。酶液对双偶氮的刚果红和曲利本蓝也有不错的脱色效果,在16 h的脱色率分别为96.76%和89.39%。对三苯基甲烷类的结晶紫的脱色效果相对较弱,在16 h的脱色率为67.31%。最终结果表明,菌株TN02A7-Lg-mnp2的酶液对多种染料都有很强的脱色能力,在染料废水处理方面具有极强的应用前景。
[Abstract]:In order to obtain the decolorization effect of (Aspergillus nidulans) manganese peroxidase transformant strain TN02A7-Lg-mnp2 on different dyes. The pH of sodium malonate buffer solution detected by enzyme activity was optimized by spectrophotometry, and the conditions of enzyme production were optimized by single factor and orthogonal test. Decolorizing effect of Congo Red, Tribon Blue and Crystal Violet. The enzyme solution had significant decolorization effect on malachite green of azo group, malachite green of triphenyl methane group and neutral red of heterocyclic group. The decolorization rates were 94.41% 91.43% and 90.00000% respectively at 1 h, and 98.36100% and 96.4747% at 16 h, respectively. The decolorization rate of the enzyme solution was 96.76% and 89.39% at 16 h, respectively. The decolorization efficiency of crystal violet for triphenylmethane was relatively weak, and the decolorization rate was 67.31 at 16 h. The final results showed that the enzyme solution of the strain TN02A7-Lg-mnp2 had strong decolorization ability for many dyes and had a strong application prospect in dye wastewater treatment.
【作者单位】：东北林业大学;
【基金】：黑龙江省自然科学基金面上项目(C2016006) 东北林业大学博士研究生自主创新基金项目(2572015AA32)
【分类号】：X172;X703

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