水稻Dof转录因子家族功能的系统研究

发布时间：2018-01-03 13:01

本文关键词：水稻Dof转录因子家族功能的系统研究　出处：《南京大学》2017年硕士论文　论文类型：学位论文

【摘要】：Dof转录因子是植物中参与多种生长发育过程调控,具有重要功能的一类蛋白质。目前对于Dof基因的研究主要集中在拟南芥中,功能可以分为组织分化、种子发育和代谢调控等方面。水稻Dof转录因子家族共含有30个基因,通过进化分析,我们可以将其分为7个亚家族,并且每个家族都存在大量特异、保守的motif。Dof基因在栽培稻和野生稻之间差异度较小,不存在明显的分化;Dof基因在栽培稻和野生稻中均存在明显的纯化选择作用,并且Dof基因在栽培稻中的群体分化程度较小。分析Dof转录因子的表达模式发现,不同亚家族的表达模式存在差异。因此根据以上系统分析,我们构建了不同的亚家族的基因功能缺失突变体,以期分析系统进化的分支与基因功能分化之间的关系。我们共构建了 30个CRISPR/Cas9的载体,对受体水稻进行农杆菌转化后,共得到12个基因的功能缺失突变体。12个基因的突变体中有10个基因表现出明显的表型,表型涉及到株高、生育期、叶形、穗形、育性等方面。其中,株高的表型散布在亚家族A、C、D、F中,而育性相关表型主要集中在亚家族C和亚家族E中,叶片相关表型集中在亚家族A和F中,而亚家族A和亚家族F影响的表型较多。结果表明,在Dof转录因子家族在演化过程中,分化出的不同分支负责调控水稻得的不同表型,并且存在重复基因的基因功能冗余情况。本研究首次利用CRISPR/Cas9技术对水稻中Dof基因进行系统的基因敲除,通过突变体表型和进化关系对水稻中的功能进行系统分析,为揭示Dof基因在植物中0作用机制和互作网络的构建奠定了基础。
[Abstract]:The transcription factor Dof is a process of growth and development regulation in various plants, a class of proteins with important functions. The present study mainly for the Dof gene in Arabidopsis, the function can be divided into tissue differentiation, seed development and metabolism regulation. Dof transcription factor family in rice contains 30 genes, through phylogenetic analysis, we it can be divided into 7 subfamilies, and there are a large number of each specific family, motif.Dof gene conserved between cultivated and wild rice difference is small, there is no obvious differentiation; Dof genes have obvious effect on selective purification of cultivated rice and wild rice, degree of population differentiation and Dof gene in small in the cultivated rice. Analysis showed that the expression pattern of Dof transcription factor, expression pattern of different subfamily differences. According to the above analysis, we construct different subfamilies The function of gene deletion mutant in the relationship between the branch and the phylogenetic analysis of gene function differentiation period. We constructed a vector of 30 CRISPR/Cas9, the receptor of Agrobacterium mediated transformation, there are 12 mutant genes deletion mutants of.12 genes in 10 genes showed significant related to the phenotype phenotype, plant height, growth period, leaf, panicle, fertility and other aspects. Among them, plant height phenotype scatter in the subfamily A, C, D, F, and fertility related phenotypes are mainly concentrated in the subfamily C and subfamily E, related phenotypes in the sub leaf the family of A and F, and the phenotype of more subfamily A and subfamily F effect. The results showed that in the Dof family of transcription factors in the evolution process of different phenotypes of the different branches are responsible for the regulation of rice, and the presence of gene function redundancy of duplicated genes. In this study, for the first time CRISPR/Cas9 technology was used to systematically knock out Dof gene in rice. The function of rice was systematically analyzed by mutational surface and evolutionary relationship, which laid the foundation for revealing the 0 mechanism of Dof gene in plant and the construction of interaction network.

【学位授予单位】：南京大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S511;Q943.2

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