中国海岸带耐盐植物DNA条形码研究

发布时间：2018-01-09 01:23

本文关键词：中国海岸带耐盐植物DNA条形码研究　出处：《华东师范大学》2017年硕士论文　论文类型：学位论文

【摘要】：以耐盐植物为主体形成的海岸带湿地生态系统,是全球单位面积服务功能最高的生态系统之一。本文对我国从辽宁到海南10个沿海省市大陆及岛屿海岸带耐盐植物广泛采样,从采集获得的样品中筛选出53科97属的116物种共562样品进行DNA条形码研究。对3个叶绿体片段rbtK、cL、trnH-psbA和1个核基因片段ITS进行扩增和测序,统计各个片段的引物通用性和序列获得率。扩增和测序方面,选择使用两对matK引物(matK-390F//matK-1326R和matK3F-KIM/matK IR-KIM)能扩增出所有类群,序列获得率达到89.32%;应用18sdir/ITS4对ITS进行扩增,序列获得率为85.59%。序列相似性分析的结果中单个片段ITS物种识别率最高(73.36%),rbcL的物种识别率最低,仅为46.41%;系统发育树的结果显示matK的物种识别率最好(82.3%)。多维度非度量分析(NMDS)和主坐标分析(PCoA)结果表明在进行海岸带区域性植物的DNA条形码研究时,叶绿体片段和核基因片段均应该考虑。综合上述研究结果,推荐联合片段ITS+matK作为中国海岸带耐盐植物DNA条形码。本文为海岸带耐盐植物研究提供了总计1939条DNA条形码基础数据,为构建耐盐植物DNA条形码数据库奠定了基础。
[Abstract]:The salt tolerant plants for wetland ecosystem in the main form of the coastal zone, is one of the global ecosystem services per unit area of the highest. The extensive sampling on China to Hainan 10 coastal provinces and cities in the mainland and islands off the coast of salt tolerant plants from Liaoning, from the collected samples screened 116 families and 97 genera of 53 species selected a total of 562 samples were DNA barcoding of 3 chloroplast fragments. RbtK, cL, trnH-psbA and 1 nuclear ITS gene fragments were amplified and sequenced. The statistics of each fragment primers universal and sequence. PCR amplification and sequencing, using two pairs of matK primers (matK-390F//matK-1326R and matK3F-KIM/matK IR-KIM) can be amplified in all groups sequence, rate 89.32%; application of 18sdir/ITS4 to ITS were amplified, sequence rate of single fragment 85.59%. sequence similarity analysis result in ITS species, the highest recognition rate (73.36%), RB Species identification of cL was the lowest, only 46.41%; phylogenetic tree species showed that the recognition rate of matK (82.3%). The best non metric multidimensional analysis (NMDS) and principal coordinate analysis (PCoA) showed that in the study of DNA barcode with Regional Coast plants, chloroplast and nuclear gene fragment all should be considered. Based on the above research results, combined with a fragment of ITS+matK as Chinese recommended coastal salt tolerant plant DNA barcoding. This paper provided a total of 1939 DNA barcode basic data for the coastal zone study on the salt tolerance of plants, for the construction of salt tolerant plants DNA laid the foundation of shape code database.

【学位授予单位】：华东师范大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：Q943.2

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