拟南芥生物节律钟相关基因LHY、CCA1和GI对其营养生长时相转变的作用

发布时间：2018-01-10 22:04

本文关键词：拟南芥生物节律钟相关基因LHY、CCA1和GI对其营养生长时相转变的作用　出处：《天津农学院》2017年硕士论文　论文类型：学位论文

【摘要】：高等植物的生长发育在整个生命周期中是一个连续的过程,包括胚胎发育和胚后发育。胚后发育也能够划分为营养发育期和生殖发育期,唯有通过营养生长时期中的幼龄期和成熟期,才会完成营养生长时相转变(vegetative phase change,VPC)。VPC是植物生长发育的一个重要生理过程,只有经历这一转变,植物体才能进入生殖生长,完成开花、结果。植物在生长发育过程中,一些表型变化、茎顶端分生组织的变化以及在分子表达水平上的不同等,都与营养生长时相转变有着密不可分的联系。最早莲座叶远轴面表皮毛可作为划分幼龄期和成熟期的一个标记;茎尖分生组织在植株生长中起着重要作用,是植物生长发育的重要途径;miR156及其靶基因家族SPLs(SQUAMOSA PROMOTER BINDING PROTEIN LIKE)中的SPL3基因在VPC中发挥重要作用。植物通过一个内在的“钟”来达到对夜长的应答进行调控的目的,在一定条件下,植物每天都能维持24 h的节律,调控这种节律的内在机制叫做生物节律钟(circadian clock)。营养生长时相转变与植物自身生物钟节律密切相关,但生物节律钟相关基因在此转变中的作用仍不明确。本研究从形态特点、解剖结构以及调控VPC的miR156和SPL3表达的分子检测等各方面分析了拟南芥(Arabidopsis thaliana)生物节律钟(circadian clock)相关基因突变体lhy、cca1、gi的营养生长时相转变特点。结果表明,突变体lhy、cca1生长周期与野生型Col-0的相差不大,但gi的生长周期比Col-0的延长;从莲座叶叶片的状态、叶片长宽比、远轴面表皮毛出现等营养生长形态特点和茎尖分生组织解剖组织特点剖析,lhy、cca1、gi突变体都延迟了营养生长时相转变。此外,RT-PCR和Q-PCR检测营养生长时相转变过程中关键调控因子miR156和SPL3的表达量结果也显示,lhy、cca1、gi突变体也延迟了营养生长时相转变。
[Abstract]:Higher plant growth and development throughout the life cycle is a continuous process, including embryo development and embryo development. Post embryonic development can also be divided into nutrition and reproductive growth period and development period, only through the period of vegetative growth in juvenile and mature period, will complete the health camp long phase transition (vegetative phase change, VPC.VPC) is one of the most important physiological processes in plant growth and development, only through this transformation, plants can enter the reproductive growth, flowering, results in plant growth and development, some phenotypic changes, changes in stem apical meristem and in the expression of the different, and vegetative growth when the phase transition is inextricably linked. The earliest rosette leaf abaxial epidermal hairs can be used as a marker of juvenile and mature period; meristem in plants plays an important role in the growth of That is an important way for plant growth and development; miR156 and its target gene family SPLs (SQUAMOSA PROMOTER BINDING PROTEIN LIKE) in the SPL3 gene play an important role in VPC. The plant through an internal clock to achieve the regulation of the night long response, under certain conditions, the plant every day maintain 24 h rhythm, the intrinsic mechanism of regulation of this rhythm is called circadian clock (circadian clock). The vegetative growth phase transition is closely related with the plant's own biological clock rhythm, but the change of circadian clock related genes in this role is not clear. This study from the aspects of morphological characteristics, anatomical structure and expression regulation VPC miR156 and SPL3 detection analysis of Arabidopsis thaliana (Arabidopsis thaliana) biological clock (circadian clock) gene mutant lhy, CCA1, GI during the vegetative growth phase transition characteristics. Results Ming, mutant lhy, CCA1 growth cycle has little difference with the wild type Col-0, but the growth cycle of GI than the Col-0 extension; from rosette leaf state, leaf length and width ratio, abaxial trichomes appeared as vegetative morphological features and meristem anatomical characteristics analysis, lhy, CCA1, GI mutants have delayed the vegetative growth phase transition. In addition, the expression of RT-PCR and Q-PCR detection when the vegetative growth phase transformation of key regulatory factor miR156 and SPL3 results also showed that lhy, CCA1, GI mutants also delayed the vegetative growth phase shift.

【学位授予单位】：天津农学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：Q943.2;Q945

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