关于CCDC-55调节拟南芥钙营养的生理和分子机制的初步探究

发布时间：2018-01-12 18:33

本文关键词：关于CCDC-55调节拟南芥钙营养的生理和分子机制的初步探究　出处：《内蒙古大学》2017年硕士论文　论文类型：学位论文

【摘要】：在植物中,Ca2+作为第二信使,不仅参与对生物胁迫和非生物胁迫的应答,还参与细胞分裂、生长、转录调节等众多进程;此外作为营养物质Ca是细胞壁的组成成分。CCDC-55是包含螺旋卷曲结构域的蛋白55的简称,此蛋白功能未知。对拟南芥CCDC-55(AT2G27280)基因缺失突变体进行Ca2+浓度梯度的表型分析,发现突变体具有低钙敏感表型。钙离子是二价阳离子,ccdc-55对其他植物必需的二价阳离子如镁、铁、锌、锰这四种矿质元素的如何响应不得而知,于是对其进行了 Mg2+、Fe2+、Zn2+、Mn2+浓度梯度的表型分析,发现突变体在低铁、高锌的培养基上与野生型拟南芥相比具有明显差异,但对突变体进行元素含量的分析结果表明,在植物体内,突变体钙、铁、锌含量与野生型植物并没有显著性差异,也就是说明该基因不参与这三种元素的储存。亚细胞定位结果显示CCDC-55定位于细胞核中,启动子活性分析结果表明CCDC-55在植物幼嫩的组织中表达。这说明CCDC-55有可能与细胞中的其他蛋白质相互作用调控植物的某些生物学过程,而在这些过程中Ca2+、Fe2+、Zn2+不可或缺。
[Abstract]:In plants, Ca 2 2, as the second messenger, not only participates in the response to biological stress and abiotic stress, but also participates in many processes, such as cell division, growth, transcription regulation and so on. In addition, as a nutrient, Ca is a component of cell wall. CCDC-55 is the abbreviation of protein 55 containing helical crimp domain. The function of this protein is unknown. The Ca2 concentration gradient phenotypic analysis of Arabidopsis thaliana CCDC-55 AT2G27280) gene deletion mutant was carried out. It was found that the mutant has a low calcium sensitive phenotype. It is not known how calcium ions are the bivalent cations necessary for other plants such as magnesium, iron, zinc and manganese. The phenotypic analysis of the concentration gradient of Mg2 Fe _ 2 and Zn _ 2O _ 2 mn _ 2 showed that the mutant had significant difference from that of wild type Arabidopsis thaliana on low iron and high zinc medium. However, the results of element analysis showed that there was no significant difference between the contents of Ca, Fe and Zn in plants and wild type plants. This means that the gene does not participate in the storage of these three elements. Subcellular localization results show that CCDC-55 is located in the nucleus. The results of promoter activity analysis showed that CCDC-55 was expressed in young tissues of plants, which suggested that CCDC-55 might interact with other proteins in cells to regulate some biological processes in plants. In these processes, Ca2 Fe _ 2 O _ 2 Zn _ 2 is indispensable.
【学位授予单位】：内蒙古大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：Q945;Q943.2

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