中国雨蛙种组物种界定及谱系地理学研究

发布时间：2018-01-28 06:06

本文关键词： 中国雨蛙种组系统发生物种界定谱系地理祖先区域重建　出处：《安徽大学》2017年硕士论文　论文类型：学位论文

【摘要】：中国雨蛙种组(Hyla chinesis-group))是从无斑雨蛙种组分离出而来,为了确定中国雨蛙种组内物种数目、成种过程以及扩散模式,本研究选用线粒体12S rRNA和ND1基因,核基因POMC作为分子标记,对中国雨蛙种组进行物种界定和谱系地理学研究。研究样品采集自大别山地区和黄山地区,并结合来自数据库的雨蛙种组的物种的序列,分别采取贝叶斯法和最大似然法对中国雨蛙种组进行系统发生分析。分析得到的系统发生树拓扑结构一致并且得到很高的置信值的支持。系统发生树中中国雨蛙种组共分为六个支系,提示中国雨蛙种组可能存在六个独立的物种。进一步的物种界定分析也支持这一结果。其中BFD的分析结果中BF值大于10,在BPP分析中结果大于0.95,均显著地支持在中国雨蛙种组中有六个物种存在。对中国雨蛙种组进行分歧时间模拟的结果显示,中国雨蛙种组起源于约18.84百万年前(处于中新世早期)。基于分歧时间,进行了祖先区域重建分析,发现中国雨蛙种组首先出现在广西-海南地区,然后从不同的扩散路线遍布全国。其中向东扩散的路线大致为:至南岭、武夷山地区,然后向北扩散到黄山周边地区,向南扩散至台湾。西向的扩散路线为:由广西-海南地区向西至云贵高原、四川盆地,进而到达秦岭山脉,最后抵达大别山地区。从整体上来看,中新世时期中纬度地区气候干旱、寒冷环境,以及在上新世时期青藏高原不断隆以及伴随而来的环境变化的后果可能是导致中国雨蛙种组形成目前谱系地理格局的主要原因。
[Abstract]:Hyla chinesis-group) was isolated from the species group of Rana chinesis-group in order to determine the species number, speciation process and diffusion pattern of Rana chensinensis. In this study, mitochondrial 12s rRNA and ND1 genes and nuclear gene POMC were used as molecular markers. The species definition and pedigree geography of Rana chensinensis were studied. The samples were collected from Dabie Mountain and Huangshan Mountains. The phylogenetic analysis of Rana chensinensis was carried out by using Bayesian method and maximum likelihood method, respectively. The phylogenetic tree topology of the phylogenetic tree was consistent and supported by a high confidence value, and the Rana chensinensis in the phylogenetic tree was supported by the phylogenetic tree. The species were divided into six branches. It is suggested that there may be six independent species in Rana chensinensis. Further species definition analysis also supports this result. The BF value of BFD analysis is greater than 10. The results of BPP analysis showed that there were six species in the group of Rana chensinensis, and the results of the diverging time simulation showed that there were six species in the group of Rana chensinensis. The species of Rana chensinensis originated from about 18.84 million years ago (located in early Miocene). Based on the time difference, the ancestor region reconstruction analysis was carried out, and it was found that the group of Rana chensinensis first appeared in Guangxi and Hainan. Then spread from different routes throughout the country. One of the routes to the east spread to the Nanling, Wuyishan region, and then spread north to the area around Huangshan. From Guangxi to Hainan to the west, to the Yungui Plateau, Sichuan Basin, then to the Qinling Mountains, and finally to the Dabie Mountains. In the Miocene, the climate was dry and cold at mid-latitude. In the Pliocene, it is possible that the rising of Qinghai-Xizang Plateau and the accompanying environmental changes may be the main reasons leading to the formation of the present phylogenetic pattern of Rana chensinensis (Rana chensinensis).
【学位授予单位】：安徽大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：Q959

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