二苯并噻吩脱硫菌的筛

发布时间：2018-03-22 08:01

  本文选题：二苯并噻吩　切入点：脱硫菌　出处：《吉林农业大学》2015年硕士论文　论文类型：学位论文

【摘要】：一提到能源,人们大多数会想到各种化石燃料,而化石燃料主要包括石油、煤炭、天然气等,这是时下世界各个国家应用最广泛的能源物质。尤其是石油的开发使用,它的贮备量与国家的命运紧密的联系起来,同时也关乎国家的工业经济命脉,所以被人们形象的誉为“工业的血液”。化石燃料的形成与结构是非常特殊而复杂的,几乎所有的化石燃料多含有硫元素,其中包括了无机硫和有机硫。无机硫多数以单质硫、硫铁矿等形式存在;有机硫主要包括硫醚、硫醇、噻吩和其他结构更为复杂的杂环化合物。而这些硫通过各种各样的方式排放到大气中,对环境造成了严重的污染,所以如何降低有机硫的排放量成为了世界各个国家最紧迫最棘手的任务之一,而二苯并噻吩(DBT)则是石油中最主要的杂环类含硫有机化合物,并且传统的脱硫工艺很难去除。所以大多数的生物脱硫技术通常以DBT为模型化合物来进行研究。本论文则以DBT作为唯一硫源,从被石油污染的土壤中驯化、分离筛选出一株具有活性高且能专一性的降解DBT的脱硫菌株5-1。通过BIOLOG鉴定结果为棒杆菌属,革兰氏染色呈阳性。通过测定菌株5-1的生长曲线可知,菌株5-1生长约为12h后开始进入了对数期,大概能持续时间达40h。此后便进入了稳定期,大约也持续40h。测定该菌株脱硫曲线可知,DBT初始浓度由0.2mmol/L降到了0.0284mmol/L,其降解率达到了85.8%。本实验论文通过八组单因素的实验对脱硫菌株5-1开始进行优化条件的培养,所得到菌株5-1的培养条件为:最佳碳源为蔗糖;最佳氮源为氯化铵;最佳C/N比为25:1;最佳培养温度为30℃;最佳初始pH值为7.0;最佳初始DBT浓度为0.2mmol/L;最佳接种量为1ml;最佳摇床转速为200r/min。
[Abstract]:When it comes to energy, most people think of all kinds of fossil fuels, and fossil fuels mainly include oil, coal, natural gas and so on. This is the most widely used energy material in various countries in the world nowadays, especially the development and use of oil. Its reserve is closely related to the fate of the country, and it is also related to the lifeblood of the country's industrial economy. Therefore, it is reputed as "the blood of industry" by people. The formation and structure of fossil fuels is very special and complex. Almost all fossil fuels contain sulfur elements, including inorganic sulfur and organic sulfur. Most inorganic sulfur exists in the form of elemental sulfur, pyrite, etc. Thiophene and other more complex heterocyclic compounds, which are emitted into the atmosphere in a variety of ways, cause serious environmental pollution. So how to reduce the emission of organic sulfur has become the most urgent and thorniest Ren Wuzhi in every country in the world, and the dibenzothiophene dithiophene (DBT) is the most important heterocyclic organic compound in petroleum. And the traditional desulfurization process is very difficult to remove. Therefore, most biological desulfurization technologies usually use DBT as a model compound. In this paper, DBT is used as the sole sulfur source, which is domesticated from the soil contaminated by oil. A desulphurized strain 5-1 with high activity and specificity for degrading DBT was isolated and screened. The result of BIOLOG identification was Corynebacterium and Gram-positive staining. The growth curve of strain 5-1 was determined. Strain 5-1 began to enter a logarithmic phase after growth of about 12 hours, which lasted about 40 hours, and then entered a stable period. The initial concentration of 0.2mmol/L decreased from 0.0284 mmol / L to 0.0284 mmol / L, and the degradation rate reached 85.8%. In this paper, eight groups of single-factor experiments were conducted to culture the desulfurized strain 5-1. The culture conditions of strain 5-1 were as follows: sucrose was the best carbon source, ammonium chloride was the best nitrogen source, the optimum C / N ratio was 25: 1, and the optimum culture temperature was 30 鈩，

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