黑脉金斑蝶bHLH转录因子家族成员鉴定与分析

发布时间：2018-03-22 09:04

  本文选题：黑脉金斑蝶　切入点：bHLH　出处：《江苏大学》2017年硕士论文　论文类型：学位论文

【摘要】：碱性螺旋-环-螺旋(basic helix-loop-helix,bHLH)转录因子广泛存在于动物、植物及真菌中,组成了一个庞大的转录因子超家族,对生物的生长发育起着重要的调控作用。动物bHLH转录因子按照功能特性分为45个家族,同时根据所结合的目标DNA元件和自身结构特点将45个家族分为A、B、C、D、E和F六个高阶组;其中A组22个家族、B组12个家族、C组7个家族、D组1个家族、E组2个家族以及F组1个家族。黑脉金斑蝶(Danaus plexippus)是鳞翅目斑蝶属的模式种,是地球上唯一一种迁徙性蝴蝶,对黑脉金斑蝶基因表达模式的研究具有重要意义与价值。分析与鉴定黑脉金斑蝶中具有哪些bHLH转录因子家族成员有助于对其迁徙行为以及昼夜节律响应等开展进一步研究。本文采用Blast搜索、多序列比对和系统发生分析等生物信息学手段对黑脉金斑蝶的bHLH转录因子进行鉴定和分类,并将其与其它昆虫的bHLH家族成员进行比较与分析,获得如下结果:1、从黑脉金斑蝶基因组中成功鉴定出55个bHLH家族成员,并将其归入40个基因家族和6个高阶组;其成员分别为A组26个,B组10个,C组11个,D组1个,E组6个以及F组1个;通过分析黑脉金斑蝶bHLH蛋白的特殊结构域,发现其成员符合动物各高阶组bHLH蛋白结构域的特征。2、在鉴定出的55个黑脉金斑蝶bHLH家族成员中,49个成员在Gen Bank蛋白序列数据库中有对应的蛋白质序列,其中24条的注释与我们的分类结果一致,8条与我们的分类结果不一致,17条注释为假定蛋白;因此,本研究为修订25条及新增6条新的黑脉金斑蝶bHLH蛋白注释信息提供了有用资料。3、黑脉金斑蝶在E12/E47、MyoD、Twist和Hand家族中分别具有2个成员,其它昆虫一般只有1个成员。黑脉金斑蝶具有3个AHR家族成员,其它昆虫一般只有2个成员。上述五个家族成员的bHLH基因分别在调控肌肉、心脏、气管的发育中发挥作用,意味着黑脉金斑蝶为了适应长距离迁徙很可能演化出了特殊的发育调控机理。4、本文还详细调查了14种昆虫bHLH基序编码区内含子的分布情况,得到了昆虫bHLH基序编码区内含子分布的总体特征,即:昆虫B组bHLH基序编码区分别与A、C、E和F组共同拥有某种内含子结构,说明其它组的bHLH编码区均不同程度地继承了B组的内含子结构;只有A组bHLH在螺旋1区存在内含子,这些内含子很可能是昆虫为了创造新的bHLH基因而插入的。上述研究结果不仅为进一步研究bHLH转录因子在黑脉金斑蝶生长发育中的调控机理建立了良好基础,也为比较分析不同昆虫bHLH基因的结构与功能以及探讨昆虫bHLH基因结构的演化机理积累了有用资料。
[Abstract]:Basic helix-loop-helix-loop-helixhhh (basic helix-loop-helixhhh) transcription factors are widely found in animals, plants and fungi, forming a large superfamily of transcription factors. Animal bHLH transcription factors were divided into 45 families according to their functional characteristics, and 45 families were divided into six higher order groups according to their target DNA elements and their own structural characteristics. Among them, group A, group B, group B, family group C, group C, group C, family group D, group D, family group E, group E, family 2 and group F. Danaus plexiippus) is a model species of the genus Lepidoptera and the only migratory butterfly on earth. It is of great significance and value to study the gene expression pattern of Erythropodes nigra. The analysis and identification of the bHLH transcription factor family members in this species is helpful to the development of its migration behavior and circadian rhythm response. This paper uses Blast search, The bHLH transcription factors were identified and classified by multiple sequence alignment and phylogenetic analysis, and compared with the bHLH family members of other insects. The following results were obtained: 1. 55 members of the bHLH family were successfully identified from the genome of Monodon nigra and classified into 40 gene families and 6 higher order groups. Its members were group A, group A, group B, group B, group 10, group C, group C, group 11, group D, group 1, group E, group E, group F, one. It was found that its members were in accordance with the characteristics of the bHLH protein domain of various higher order groups of animals. Among the 55 identified members of the bHLH family, 49 members had corresponding protein sequences in the Gen Bank protein sequence database. Of these, 24 notes are consistent with our classification results and 8 are inconsistent with our classification results. 17 notes are hypothetical proteins; therefore, This study provides useful data for revising 25 and 6 new bHLH protein annotation information. The E12 / E47 MyoDU Twist and Hand families have two members, respectively, in the E12 / E47 MyoDU Twist family and in the E12 / E47 MyoDU Twist family. The other insects usually have only one member. There are three members of the AHR family and two members of the other insects. The bHLH genes of the five family members play a role in regulating the development of muscle, heart and trachea, respectively. In order to adapt to long distance migration, a special developmental regulation mechanism. 4. The distribution of intron in bHLH motif coding region of 14 species of insects was investigated in detail. The general characteristics of intron distribution in insect bHLH motif coding region were obtained, that is, insect group B bHLH motif coding region shared a certain intron structure with group A Con E and group F, respectively. The results show that the bHLH coding regions of other groups inherit the intron structure of group B to some extent, only group A bHLH has intron in helix 1 region. These introns were probably inserted by insects in order to create new bHLH genes. These results not only provide a good basis for further study on the regulation mechanism of bHLH transcription factors in the growth and development of Erythropodes nigra. It also provides useful data for comparing and analyzing the structure and function of different insect bHLH genes and for exploring the evolution mechanism of insect bHLH gene structure.
【学位授予单位】：江苏大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：Q963

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