核苷酸转移酶在拟南芥非生物胁迫应答中的功能研究

发布时间：2018-04-14 18:16

本文选题：NTP蛋白 + 胁迫　；参考：《深圳大学》2017年硕士论文

【摘要】：高盐、干旱、高温或低温等非生物胁迫严重影响现代农业的可持续发展。而生物本身可以通过调节RNA的丰度与RNA的稳定性来提高对逆境的耐受性。NTP(核苷酸转移酶,Nucleotidyltransferase)是一类包含Poly(A)聚合酶和NT结构域(末端尿苷酸转移酶,Nucleotidyltransferase)蛋白,它能在RNA的末端非模板地添加核苷酸,从而改变RNA的稳定性。影响RNA在体内的丰度。虽然对植物中个别的NTP蛋白已经有初步的研究,但是大多数NTP蛋白的功能还尚不清楚。本实验首先通过生物信息学的方法分析了拟南芥中10种NTP的蛋白序列及结构特点,NTP蛋白虽然都拥有相同的结构域的非稳定蛋白,但其酸碱性差异较大;NTP蛋白与其他蛋白有明显的互作关系,并且10种NTP蛋白都与CSDP1直接互作,CSDP1是一种胁迫相关基因,直接参与对胁迫的应答;10种基因中均存在胁迫元件,表明NTP蛋白可能参与各种胁迫应答,并在胁迫响应中发挥功能。其次本实验通过荧光定量PCR的方法,对10种NTP基因分别进行了盐、干旱、冷、热等胁迫下的诱导表达分析。发现HESO1、NTP6和NTP7是受盐、干旱、冷、热等胁迫诱导。最后通过3种突变体在盐及干旱中的表型分析,发现在盐胁迫下heso1、ntp6和ntp7萌发率、变绿子叶率、根长及生物量均低于野生型植株(Col-WT),说明heso1、ntp6和ntp7对盐胁迫的耐受性下降;在干旱胁迫下,heso1、ntp6和ntp7在失水后植物组织受损较Col-WT更严重,说明heso1、ntp6和ntp7对干旱胁迫的耐受性也下降。综上所述,本研究发现NTP家族中的HESO1、NTP6和NTP7受到非生物胁迫的诱导表达,并对拟南芥的非生物胁迫耐受性产生重要影响,说明HESO1、NTP6和NTP7可能参与植物对环境胁迫的反应。
[Abstract]:Abiotic stresses such as high salt, drought, high temperature or low temperature seriously affect the sustainable development of modern agriculture.By regulating the abundance of RNA and the stability of RNA, the organism can improve its tolerance to stress. NTP (nucleotidyltransferase) is a class of nucleotidyltransferase and nucleotidyltransferase protein.It can change the stability of RNA by adding nucleotides to the end of RNA without template.The abundance of RNA in vivo was affected.Although a few NTP proteins have been studied in plants, the function of most NTP proteins remains unclear.In this study, the sequence and structural characteristics of 10 NTP proteins in Arabidopsis thaliana were analyzed by bioinformatics.However, there was a significant difference between NTP protein and other proteins, and all 10 NTP proteins were directly interacting with CSDP1. CSDP1 was a stress related gene, and there were stress elements in all 10 genes directly involved in the response to stress.The results suggest that NTP protein may participate in various stress responses and play a role in stress response.Secondly, the induced expression of 10 NTP genes under salt, drought, cold and heat stress were analyzed by fluorescence quantitative PCR.It was found that HESO1 NTP6 and NTP7 were induced by salt, drought, cold and heat stress.Finally, the phenotypic analysis of three mutants in salt and drought showed that the germination rate, green cotyledon rate, root length and biomass of Heso1ntp6 and ntp7 under salt stress were lower than those of wild type plant Col-WTP, which indicated that the tolerance of Hheso1ntp6 and ntp7 to salt stress was decreased.Hesheso1ntp6 and ntp7 were damaged more seriously than Col-WT after water loss, which indicated that the tolerance of Hesheso1ntp6 and ntp7 to drought stress was also decreased.In conclusion, we found that HESO1 NTP6 and NTP7 in NTP family were induced by abiotic stress and had an important effect on abiotic stress tolerance of Arabidopsis thaliana, suggesting that HESO1 NTP6 and NTP7 may be involved in plant response to environmental stress.
【学位授予单位】：深圳大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：Q943.2;Q945.78

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