ABA调控拟南芥种子休眠的相关机制探究

发布时间：2018-04-17 16:03

本文选题：种子休眠 + 脱落酸　；参考：《重庆师范大学》2017年硕士论文

【摘要】：种子作为农业生产中最基本、最重要的生产资料,植株的生长发育和产量很大程度上取决于种子的质量。种子的萌发和休眠在植物的个体生长发育中处于初期阶段,是一个至关重要的过程。探究种子休眠机制对提高种子活力,增加幼苗的抗逆性、出苗率以及作物产量都有着重大意义。植物激素脱落酸(ABA)能够抑制种子的萌发,促进种子的休眠,其中ABI1基因具有非常重要的作用。ABI1基因在序列中第180处甘氨酸突变为天冬氨酸后,导致种子对ABA不敏感。在野生型的拟南芥中非特异性表达该突变型ABI1基因,可消除种子对ABA的响应。但这一影响是否存在组织特异性目前尚不明确。本研究通过构建组织特异性启动子和植物GAL4/UAS异位表达技术在拟南芥的不同组织细胞中特异性表达abi1,利用不同浓度的ABA进行萌发实验,探究ABA在种子休眠的分子机制。研究结果如下:(1)根尖干细胞特异表达的abi1基因的转基因植株种子萌发率高于野生型但萌发速率没有明显的增加。(2)茎尖干细胞特异表达的abi1基因的转基因植株表现出对ABA更加敏感,萌发率不到40%。(3)J2133驱动的abi1表达导致种子对ABA的敏感性降低。
[Abstract]:As the most basic and important means of production in agricultural production, the growth, development and yield of plants depend to a great extent on the quality of seeds.Seed germination and dormancy is a very important process in the process of individual growth and development of plants.It is of great significance to explore the mechanism of seed dormancy to improve seed vigor, increase seedling resistance, seedling emergence rate and crop yield.The plant hormone abscisic acid (Aba) can inhibit seed germination and promote seed dormancy. ABI1 gene plays a very important role. ABI1 gene mutates to aspartic acid at 180th point in the sequence, which results in the seed being insensitive to ABA.The nonspecific expression of the mutant ABI1 gene in wild type Arabidopsis thaliana could eliminate the response of seeds to ABA.However, it is not clear whether this effect has tissue specificity.In this study, we constructed tissue specific promoter and plant GAL4/UAS heterotopic expression techniques to express abi1 in different Arabidopsis tissues and cells, and used different concentrations of ABA to carry out germination experiments to explore the molecular mechanism of ABA in seed dormancy.The results are as follows: (1) the seed germination rate of abi1 gene specifically expressed by root tip stem cells is higher than that of wild type, but the germination rate of abi1 gene expressed by stem tip stem cells is not significantly increased. 2) the expression of transgenic plants of abi1 gene specifically expressed by stem tip stem cells is higher than that of wild type.More sensitive to ABA,Abi1 expression, which was less than 40%.(3)J2133 driven by germination rate, reduced the sensitivity of seeds to ABA.
【学位授予单位】：重庆师范大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：Q943.2

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