对海兔位置移动网络里蠕动波的神经控制研究

发布时间：2018-05-31 06:26

本文选题：海兔 + 位置移动　；参考：《南京大学》2017年硕士论文

【摘要】：神经活动的蠕动波(rolling wave)所引起的位置移动是广泛存在于脊椎和无脊椎动物中的节律性运动,而控制这种运动的神经环路被称为中枢模式发生器。在海兔(Aplysia californica)中,以蠕动波所产生的逃跑位置移动行为是研究位置移动中枢模式发生器的绝佳模型。与那些由多个体节神经网络或体节神经节调控的位置移动不同,海兔的中枢模式发生器存在于单一的脚神经节中。根据先前的研究,我们将工作的重点放在了腹侧脚神经节上。我们用色素回填定位了右侧脚神经节腹面p1神经根部的一个细胞团,并且用半离体实验确认了它们可以引起肌肉收缩,进一步定性为运动神经元。用电生理记录的方法同时进行细胞内记录和细胞外神经记录,发现了运动神经元兴奋发放模式与运动指示器PPCN运动神经存在时相差,并将所记录的细胞与PPCN的时相关系、细胞间电偶联做了总结,发现时相相近的运动神经元互相电偶联。同时在p7根部记录到与单一时相运动神经元电偶联的中间神经元。这些不同时相节律性发放的神经元有可能是位置移动行为蠕动波的神经基础,而电偶联现象可能是时间节律性发放的理论基础。依据色素回填的实验,我们猜测有协调型中间神经元的存在。我们的研究对海兔蠕动波的中枢控制有了一定初步的了解,为将来更进一步的研究打下了坚实基础。
[Abstract]:The peristaltic wave rolling wave caused by neural activity is a rhythmic movement widely found in vertebrates and invertebrates. The neural loop that controls this movement is called the central pattern generator. In the sea rabbit Aplysia californica, the flight position movement behavior caused by peristaltic waves is an excellent model for studying the central pattern generator of position movement. The central pattern generator of sea rabbits is located in a single ganglion instead of those that are regulated by multiple ganglion networks or ganglion. Based on previous studies, we focused our work on the ventral ganglion of the foot. We have located a cell mass in the root of the ventral face of the right foot ganglion by pigment backfilling. The semi-isolated experiments have confirmed that they can induce muscle contraction and are further characterized as motoneurons. The electrophysiological recording method was used to record both intracellular and extracellular nerves. It was found that the excitatory mode of motor neurons was different from that of PPCN motor nerves, and the time-dependent relationship between the recorded cells and PPCN was found. The results of intercellular electrocoupling showed that motoneurons with similar phases were electrically coupled with each other. At the same time, the interneuron electrically coupled with a single temporal motor neuron was recorded at the root of p7. These rhythmic neurons may be the neural basis of peristaltic waves, while the electrocoupling phenomenon may be the theoretical basis of temporal rhythmic distribution. Based on the experiment of pigment backfilling, we speculate the existence of coordinated intermediate neurons. Our study provides a preliminary understanding of the central control of peristaltic waves in sea rabbits, which lays a solid foundation for further research.
【学位授予单位】：南京大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：Q42

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