沉积物陆丹氏菌（Loktanella sediminum sp. nov.）等3株东海新菌的分类鉴定

发布时间：2018-06-07 19:30

本文选题：ECS + 海洋细菌　；参考：《中国海洋大学》2015年硕士论文

【摘要】：中国东海(East China Sea, ECS)是西太平洋的一个边缘海,位于我国大陆东侧,大致范围在2133°N、117-131°E之间。东海拥有丰富的海洋生物资源,而且还是中国油气资源储备的战略要地。海洋微生物所具有的独特的生理代谢特征及其生态功能,具有非常重要的研究意义和广泛的应用前景。对这些微生物进行系统地分类和鉴定,可以丰富海洋微生物种类,为研究其在生物地球化学循环中的作用、开发生物活性物质奠定基础。本论文利用多相分类法对从东海表层海水及表层沉积物中分离得到的三株海洋新菌进行了鉴定,分别命名为：沉积物陆丹氏菌(Loktanella sediminum sp. nov.)、海水纪氏菌(Jiella aquimaris gen. nov., sp. nov.)和耐盐橙芽孢杆菌(Aurantibacillus halotolerans gen. nov., sp. nov.)。1.沉积物陆丹氏菌(Loktanella sediminum sp. nov.) S3B03T的分类鉴定：S3B03T属于a-变形菌纲,陆丹氏菌属的一个新种,命名为沉积物陆丹氏菌(Loktanella sediminum sp. nov.),标准菌株为S3B03T (=JCM 30120T= DSM 28715T=MCCC 1K00257T)。从遗传学特征来看,S3B03T的16S rRNA基因序列与Loktanella litorea KCTC 23883T的相似度最高,为96.16%,与Loktanella属其他种的相似度在92.99-95.38%之间。S3B03T细胞为革兰氏阴性,短杆状,无鞭毛,胞内贮存有PHB (poly-p-hydroxybutyrate)颗粒。基因组DNA G+C含量为57.6 mol%,主要的呼吸醌为泛醌-10(Q-10),主要的脂肪酸(含量超过10%)为summed feature 8 (C18:ω7c and/or C18:1ω6c),主要的极性脂为磷脂酰甘油(PG)、双磷脂酰甘油(DPG)、卵磷脂(PC)、磷脂酰乙醇胺(PE)和两种未鉴定的极性脂(L1、L2)。2.海水纪氏菌(Jiella aquimaris gen. nov., sp. nov.) LZB041T的分类鉴定：LZB041T属于α-变形菌纲,橙单胞菌科的一个新属新种,命名为海水纪氏菌(Jiella aquimaris gen. nov., sp. nov.),标准菌株为LZB041T(=JCM 30119T=MCCC 1K00255T)。遗传学分析表明,LZB041T与橙单胞菌科的菌亲缘关系最近,其16S rRNA基因序列与Aurantimonas和Aureimonas属的相似度比较接近,分别为96.0-96.4%和94.5-96.0%。LZB041T细胞为革兰氏阴性,杆状,周生鞭毛。基因组DNA G+C含量为71.3 mol%,主要的呼吸醌为泛醌-10(Q-10),主要的脂肪酸(含量超过10%)为summed feature 8(C18:1ω7c and/or C18:1ωe6c)和cyclo-C19:0ω8c,主要的极性脂为磷脂酰甘油(PG)、双磷脂酰甘油(DPG)、卵磷脂(PC)、磷脂酰乙醇胺(PE)、磷脂酰单甲基乙醇胺(PME)、一种未鉴定的氨基脂(AL)、一种未鉴定的磷脂(PL)和一种未鉴定的极性脂(L)。3.耐盐橙芽孢杆菌(Aurantibacillus halotolerans gen. nov., sp. nov.) S1203T的分类鉴定：S1203T属于厚壁菌门,芽孢杆菌科的一个新属新种,命名为耐盐橙芽孢杆菌(.Aurantibacillus halotolerans gen. nov., sp. nov.),标准菌株为S1203T (= JCM 30067T= DSM 28697T= MCCC 1K00259T)。从遗传学特征来看,S1203T与芽孢杆菌科的菌亲缘关系最近,其16S rRNA基因序列与Bacillus属的相似度为86.2-93.8%。S1203T细胞为革兰氏阳性,杆状,周生鞭毛,且产生芽孢。基因组DNA G+C含量为47.7 mol%,主要的呼吸醌为甲基萘醌-7(MK-7),细胞壁特征二氨基酸为meso-diaminopimelic acid (meso-Dpm),主要的脂肪酸(含量超过10%)为anteiso-C15:0、iso-C15:0和C16：0,主要的极性脂为磷脂酰甘油(PG)、双磷脂酰甘油(DPG)、一种未鉴定的糖脂(GL)和一种未鉴定的磷脂(PL)。
[Abstract]:The East China Sea (ECS) is a marginal sea in the Western Pacific Ocean, located on the eastern side of the mainland of China, roughly between 2133 and 117-131 E. The East China Sea has abundant marine biological resources, and it is also the strategic place of China's oil and gas reserves. The systematic classification and identification of these microbes can enrich the species of marine microorganisms, and lay the foundation for the study of their role in the biogeochemical cycle and the development of bioactive substances. In this paper, the polyphase classification method was used to deposit surface water and surface layer from the East China Sea. Three new marine strains isolated from the samples were identified as Loktanella sediminum sp. Nov., Jiella aquimaris Gen. Nov., sp. Nov. and Bacillus salioris (Aurantibacillus halotolerans). The classification and identification of sp. Nov.) S3B03T: S3B03T belongs to the a- Proteus, a new species of the genus Lu Dan, named for the Loktanella sediminum sp. Nov., and the standard strain is S3B03T (=JCM 30120T=). The similarity of 883T is the highest, 96.16%. The similarity degree between Loktanella and other species is gram negative, short rod like, non flagellate and PHB (poly-p-hydroxybutyrate) granules in the cell. The content of DNA G+C in the genome is 57.6 mol%, the main respiratory quinone is ubiquinone -10 (Q-10) and the main fatty acid (more than 10%). ) for summed feature 8 (C18: Omega 7C and/or C18:1 Omega 6c), the main polar lipids are phosphatidylglycerol (PG), diphosphatidic glycerol (DPG), lecithin (PC), phosphatidyl ethanolamine (PE) and two unidentified polar lipids. A new genus of the genus orangiaceae, named Jiella aquimaris Gen. Nov. (sp. Nov.), and the standard strain is LZB041T (=JCM 30119T=MCCC 1K00255T). Genetic analysis shows that LZB041T is closely related to the bacteria in the family of orangiaceae. 96.0-96.4% and 94.5-96.0%.LZB041T cells are gram-negative, rod-shaped and peripheral flagellum, respectively. The content of genomic DNA G+C is 71.3 mol%, the main respiratory quinones are ubiquinone -10 (Q-10), and the main fatty acids (more than 10%) are summed feature 8 (C18:1 Omega 7C and/or) and omega, and the main polar lipids are Phosphatidylglycerols (PG), diphosphatidyl glycerol (DPG), lecithin (PC), phosphatidyl ethanolamine (PE), phosphatidyl monomethyl ethanolamine (PME), an unidentified amino fat (AL), an unidentified phospholipid (PL) and an unidentified polar lipid (L).3. tolerant orange bacillus (Aurantibacillus halotolerans Gen.) A new genus and new species of the family bacilliaceae, named.Aurantibacillus halotolerans Gen. Nov. (sp. Nov.), and the standard strain S1203T (= JCM 30067T= DSM 28697T= MCCC). The similarity of 86.2-93.8%.S1203T cells with Bacillus is gram positive, rod like, peripheral flagellum and spore. The DNA G+C content of the genome is 47.7 mol%, the main respiratory quinone is methylnaphthone -7 (MK-7), the cell wall characteristic is meso-diaminopimelic acid (meso-Dpm), and the main fatty acid (more than 10%) is anteiso-C15. 0, iso-C15:0 and C16:0, the main polar lipids are phosphatidylglycerol (PG), double phosphatidylglycerol (DPG), an unidentified glycolipid (GL) and an unidentified phospholipid (PL).
【学位授予单位】：中国海洋大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：Q939

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