荒漠植物的多功能PGPR的分离鉴定及其功能特性研究

发布时间：2018-07-04 12:41

  本文选题：荒漠 + 多功能植物根际促生菌　； 参考：《内蒙古农业大学》2017年硕士论文

【摘要】：植物根际促生细菌(Plant Groeth Promoting Rhizobacteria,PGPR)是植物根际存在的一类能够促进和保护植物生长发育的有益细菌,可以促使荒漠植物适应其所在恶劣生态环境,并具有应用于生态农业的潜力。然而,有关荒漠植物根际土中的PGPR研究还较少,相关类群组成和功能特性还不清楚。本研究采集了生长于内蒙古巴丹吉林、吉兰泰、腾格里沙漠和巴拉贡等荒漠中的梭梭(Haloxylon ammodendron)、沙冬青(Ammopiptarthus mongolicus)、白刺(Nitraria tangutorum)、盐爪爪(Kalidiumm foliatum)、红砂(Reaumuria soongoricaa)等几种常见植物的根际土混合样,应用传统涂布划线方法,分离筛选了具有固氮、溶磷、产铁载体、产IAA及产ACC脱氨酶功能的根际细菌,基于16SrDNA基因序列技术进行了初步分类鉴定。结果表明,共获得472株PGPR,其中用Ashby培养基筛选得到固氮菌有191株,优势菌门为变形菌门(Proteobacteria)占85.8%,用AZO培养基筛选得到固氮菌149株,优势菌门为放线菌门(Actinobacteria)占43.7%,用有机磷培养基筛选得到溶有机磷菌株53株,优势菌门为放线菌门(Actinobacteria)占69.8%,溶用NBRIP培养基筛选得到无机磷菌株79株,优势菌门为放线菌门(Actinobacteria).占59.5%。将得到的菌株进行促生能力分析,最终具有固氮能力,溶磷能力,产铁载体,产IAA及产ACC脱氨酶能力的细菌有9株,编号分别为1、58、B58、B70、界64、Y16-1、Y18、Y34、Y43,经PCR扩增16SrDN贴,测定其基因序列与EZ-BioCloud数据库中同源序列进行比对,同源性分别与ArthrobacternitroguajacolicusG2-1T、PseudomonasbrassicacearumATCC 49054T、Rhizobium radiobacter ATCC 19358TT、Pantoea conspicua LMG 24534T、Rhodococcus marinonascens DSM 43752T、Gordonia terraeNBRC 100016T、Arthrobbacter nicotianae DSM 20123T、Bacillus cereus ATCC 14579T、Gordonia effusa IFM 10200T最为接近,在99-100%之间。
[Abstract]:Plant Groeth promoting bacteria (PGPR) is a kind of beneficial bacteria which can promote and protect the growth and development of plants in the rhizosphere of plants. It can promote desert plants to adapt to its harsh ecological environment and have the potential to be applied to ecological agriculture. However, there are few studies on PGPR in the rhizosphere of desert plants, and the composition and functional characteristics of related groups are not clear. In this study, the rhizosphere soil mixed samples of Haloxylon ammodendron), (Ammopiptarthus mongolicus), (Ammopiptarthus mongolicus),) grown in Badain Jilin, Jilantai, Tenggari and Barragon deserts in Inner Mongolia were collected, such as Kalidiumm foliatum), red sand (Reaumuria soongoricaa) and so on. Rhizosphere bacteria with nitrogen-fixing, phosphorus-soluble, iron-producing carrier, IAA production and ACC deaminase production were isolated and screened by traditional coating method. The bacteria were preliminarily classified and identified based on 16s rDNA gene sequence technique. The results showed that 472 strains of PGPRs were obtained, of which 191 strains were screened by Ashby medium, 85.8% by Proteobacteria, 149 strains were screened by AZO medium. The dominant phylum was actinomycetes (Actinobacteria) accounting for 43.7%. 53 strains of organophosphorus strains were screened by organic phosphorus medium, 69.8% of them by (Actinobacteria), 79 strains of inorganic phosphorus strains were screened by using NBRIP medium, and 79 strains were screened as (Actinobacteria). Of actinomycetes by using organophosphorus medium. 59.5%. 9 strains of bacteria with nitrogen fixation ability, phosphorus solubilization ability, iron production carrier, IAA production ability and ACC deaminase production capacity were identified as 1t58.B58B70, and 64FY16-1OY18OY34-Y43, respectively, and 16SrDN stickers were amplified by PCR, and the results showed that the bacteria had the ability to fix nitrogen, dissolve phosphorus, produce IAA, and produce ACC deaminase, respectively. 娴嬪畾鍏跺熀鍥犲簭鍒椾笌EZ-BioCloud鏁版嵁搴撲腑鍚屾簮搴忓垪杩涜�屾瘮瀵�,鍚屾簮鎬у垎鍒�涓嶢rthrobacternitroguajacolicusG2-1T,PseudomonasbrassicacearumATCC 49054T,Rhizobium radiobacter ATCC 19358TT,Pantoea conspicua LMG 24534T,Rhodococcus marinonascens DSM 43752T,Gordonia terraeNBRC 100016T,Arthrobbacter nicotianae DSM 20123T,Bacillus cereus ATCC 14579T,Gordonia effusa IFM 10200T鏈，

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