植物乳杆菌降脂软胶囊配方优化及功能性研究
发布时间：2018-08-19 07:19
【摘要】：血脂异常易诱发心梗、冠心病和脑卒中等心脑血管疾病。因此,研发一种预防和缓解血脂异常的功能性食品是非常必要的。本研究以课题组自行分离的高效降胆固醇植物乳杆菌M1-UVs29为出发菌株,以植物乳杆菌M1-UVs29和米糠油为主要功效成分,研制一款新型降脂软胶囊,并通过体外与体内试验,对降脂软胶囊进行功能性评价,主要研究结果如下:(1)依次利用Plackett-Burman,最陡爬坡和Box-Benhnken对植物乳杆菌M1-UVs29冷冻干燥保护剂成分进行优化。确定保护剂的最佳配比为:脱脂乳13.00%(w/v),海藻糖7.60%(w/v),山梨醇2.70%(w/v),该条件下菌粉存活率达86.28%。添加和未添加保护剂的植物乳杆菌M1-UVs29,在模拟人工胃液、人工肠液、储存时间和热处理条件下的存活能力差异显著(P0.05),表明该保护剂具有较好的保护能力。(2)将经过保护剂包埋的植物乳杆菌M1-UVs29和米糠油结合制成具有降脂功能的软胶囊。进行单因素优化确定降脂软胶囊内容物最佳配比为:植物乳杆菌菌粉14.00%、低聚果糖14.00%、米糠油69.97%、蜂蜡2.00%和抗氧化剂0.03%,该软胶囊感官指标、理化指标、卫生指标、加速试验和稳定性试验符合质量标准,说明该配方符合降脂软胶囊工业化生产要求。(3)降脂软胶囊体外功能评价。以软胶囊内容物体外降胆固醇以及脂肪吸附能力作为检测指标,结果表明:体外降胆固醇能力最高可达到59.23%,脂肪吸附能力最高可达到21.14%,说明该软胶囊内容物在体外试验中可以起到降脂、降胆固醇的功效。(4)降脂软胶囊体内功能评价。以该软胶囊内容物作为原料,C57BL/6J小鼠为动物试验对象。以降脂软胶囊PH、PM、PL组与FAT组相比,通过对C57BL/6J小鼠的体重、各部分脂肪与体重比、内脏与体重比、肠道菌群、血清中TC、TG、LDL、HDL、血清中INS、TNF-α、TBARS、肝脏中TG、肝脏中FAS和CPT-1等指标分析,结果表明降脂软胶囊各剂量组与FAT组相比,差异显著(P0.05),肝脏组织病理分析发现该降脂软胶囊可以改善高脂饮食造成的血脂异常症状。综上所述,该降脂软胶囊配方合理,植物乳杆菌M1-UVs29活菌数符合国家质量标准,稳定性良好,具有预防和改善高血脂的作用。
[Abstract]:Dyslipidemia can easily induce myocardial infarction, coronary heart disease and stroke and other cardiovascular and cerebrovascular diseases. Therefore, it is necessary to develop a functional food to prevent and alleviate dyslipidemia. In this study, a new antilipidemic soft capsule was developed with Lactobacillus plantarum M1-UVs29 isolated by our team as the starting strain, Lactobacillus plantarum M1-UVs29 and rice bran oil as the main functional components, and tested in vitro and in vivo. The main results were as follows: (1) Plackett-Burman, steepest climbing slope and Box-Benhnken were used to optimize the composition of M1-UVs29 freeze-drying protectant for Lactobacillus plantarum. The optimum ratio of protective agent was determined as follows: skim milk 13.00% (w / v), trehalose 7.60% (w / v), sorbitol 2.70% (w / v), and the survival rate of bacteria powder reached 86.28%. Lactobacillus plantarum M1-UVs29, added and not added, in simulated artificial gastric juice, artificial intestinal fluid, There were significant differences between storage time and survival ability under heat treatment (P0.05), which indicated that the protective agent had better protective ability. (2) soft capsules with lipid-lowering function were made from Lactobacillus plantarum M1-UVs29 and rice bran oil. The optimum proportion of contents of Jiangzhi soft capsule was determined as follows: Lactobacillus plantarum powder 14.00, fructose 14.00, rice bran oil 69.97, beeswax 2.00% and antioxidant 0.03. The accelerated test and stability test accord with the quality standard, which shows that the formula meets the requirement of industrial production of Jiangzhi soft capsule. (3) the function evaluation of Jiangzhi soft capsule in vitro. The content of soft capsule was used as the index of cholesterol lowering and fat adsorption in vitro. The results showed that the ability of lowering cholesterol in vitro was the highest 59.23 and the ability of fat adsorption was 21.14. The results showed that the content of the soft capsule could reduce lipid and reduce cholesterol in vitro. (4) the function of Jiangzhi soft capsule in vivo was evaluated. The soft capsule contents were used as raw material for C57BL / 6J mice. Compared with FAT group, the weight of C57BL/6J mice, the ratio of fat to body weight, the ratio of viscera to body weight, intestinal flora, serum TCG-TGG-LDLL-HDL, serum INSTNF- 伪 TBARS, liver, FAS and CPT-1 were analyzed. The results showed that there were significant differences between the different dosage groups of Jiangzhi soft capsule and FAT group (P0.05). The pathological analysis of liver tissue showed that Jiangzhi soft capsule could improve the hyperlipidemia symptoms caused by high-fat diet. To sum up, the formula of Jiangzhi soft capsule is reasonable, the number of live bacteria of Lactobacillus plantarum M1-UVs29 accords with the national quality standard, the stability is good, and it has the function of preventing and improving hyperlipidemia.
【学位授予单位】：黑龙江八一农垦大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：TS201.3;TS218
本文编号：2191050
[Abstract]:Dyslipidemia can easily induce myocardial infarction, coronary heart disease and stroke and other cardiovascular and cerebrovascular diseases. Therefore, it is necessary to develop a functional food to prevent and alleviate dyslipidemia. In this study, a new antilipidemic soft capsule was developed with Lactobacillus plantarum M1-UVs29 isolated by our team as the starting strain, Lactobacillus plantarum M1-UVs29 and rice bran oil as the main functional components, and tested in vitro and in vivo. The main results were as follows: (1) Plackett-Burman, steepest climbing slope and Box-Benhnken were used to optimize the composition of M1-UVs29 freeze-drying protectant for Lactobacillus plantarum. The optimum ratio of protective agent was determined as follows: skim milk 13.00% (w / v), trehalose 7.60% (w / v), sorbitol 2.70% (w / v), and the survival rate of bacteria powder reached 86.28%. Lactobacillus plantarum M1-UVs29, added and not added, in simulated artificial gastric juice, artificial intestinal fluid, There were significant differences between storage time and survival ability under heat treatment (P0.05), which indicated that the protective agent had better protective ability. (2) soft capsules with lipid-lowering function were made from Lactobacillus plantarum M1-UVs29 and rice bran oil. The optimum proportion of contents of Jiangzhi soft capsule was determined as follows: Lactobacillus plantarum powder 14.00, fructose 14.00, rice bran oil 69.97, beeswax 2.00% and antioxidant 0.03. The accelerated test and stability test accord with the quality standard, which shows that the formula meets the requirement of industrial production of Jiangzhi soft capsule. (3) the function evaluation of Jiangzhi soft capsule in vitro. The content of soft capsule was used as the index of cholesterol lowering and fat adsorption in vitro. The results showed that the ability of lowering cholesterol in vitro was the highest 59.23 and the ability of fat adsorption was 21.14. The results showed that the content of the soft capsule could reduce lipid and reduce cholesterol in vitro. (4) the function of Jiangzhi soft capsule in vivo was evaluated. The soft capsule contents were used as raw material for C57BL / 6J mice. Compared with FAT group, the weight of C57BL/6J mice, the ratio of fat to body weight, the ratio of viscera to body weight, intestinal flora, serum TCG-TGG-LDLL-HDL, serum INSTNF- 伪 TBARS, liver, FAS and CPT-1 were analyzed. The results showed that there were significant differences between the different dosage groups of Jiangzhi soft capsule and FAT group (P0.05). The pathological analysis of liver tissue showed that Jiangzhi soft capsule could improve the hyperlipidemia symptoms caused by high-fat diet. To sum up, the formula of Jiangzhi soft capsule is reasonable, the number of live bacteria of Lactobacillus plantarum M1-UVs29 accords with the national quality standard, the stability is good, and it has the function of preventing and improving hyperlipidemia.
【学位授予单位】：黑龙江八一农垦大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：TS201.3;TS218
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