三维旋转生物反应器设计及其流体剪切力分析

发布时间：2018-10-31 16:23

【摘要】：传统的微重力研究只能在空间站、失重飞机、落塔等空间设备上进行,但均难以避免模拟时间短,成本高的代价。旋转式反应器模拟微重力效应原理与空间失重效应类似,因此被国际上微重力实验室所采用,甚至医学、遗传学、生物学等领域专家学者运用该设备来观察微生物、动植物体的微重力应激反应。现有的旋转式生物反应器都面临实现培养液供给和控制流动剪切力之间矛盾问题,培养液供给不可避免地带来了剪切力作用,剪切力作用不利于植物生长。本课题来源于福建省农科院生态研究所承担的国家高技术研究发展项目(2010AA743042),福建省自然科学基金项目(2011J01101)。以三维旋转生物反应器为研究对象,立足于培养液供给和流动剪切力这2个基点出发,研制一种能够模拟植物微重力效应三维旋转反应器,本文主要完成如下工作：1)运用功能分析法对三维旋转反应器进行功能元分解和形态学矩阵重组设计,得出多种解法并选取最优解；2)测量304不锈钢内壁实验组与对照组2种条件下栽培盘的光照强度,验证304不锈钢内壁能否满足植物光源需求,此外,运用ANSYS对旋转轴进行结构分析；3)应用FLUENT软件对二维、三维旋转生物反应器栽培盘流体分析,比较二维、三维旋转生物反应器水培植物的剪切力整体水平对比,结果表明,三维旋转反应器较小的剪切力水平更适合栽培植物,研究离轴位置与流体剪切力关系,得出结论,离轴距离越近,剪切力峰值越小。样机完成后应用于多种植物体(红萍、苋菜)进行旋转培育实验,实验结论表明：说明三维旋转生物反应器对红萍根系生长有着显著影响；此外,苋菜株高实验结果表明,栽培位置X轴为基准旋转轴,离轴距离越小,幼苗的株高在同等培育条件下略高与对照,这种方式宏观上亦验证了FLUENT流体分析的剪切力理论。
[Abstract]:Traditional microgravity research can only be carried out on space equipment such as space station, weightlessness aircraft and landing tower, but it is difficult to avoid the cost of short simulation time and high cost. The principle of simulating microgravity effects in rotary reactors is similar to that of space weightlessness, so it has been used in microgravity laboratories around the world, and even experts and scholars in medicine, genetics, biology and other fields have used this equipment to observe microbes. The microgravity stress response of animals and plants. The existing rotary bioreactor is confronted with the contradiction between the supply of culture medium and the control of flow shear force, which inevitably brings about shear force, which is not conducive to plant growth. This project comes from the National High Technology Research and Development Project (2010AA743042) and the Natural Science Foundation (2011J01101) of Fujian Provincial Academy of Agricultural Sciences. A three-dimensional rotating bioreactor was developed based on the two basis points of medium supply and flow shear force, which can simulate the microgravity effect of plants. The main work of this paper is as follows: 1) the functional element decomposition and morphological matrix reorganization design of 3D rotating reactor are carried out by using functional analysis method, and various solutions are obtained and the optimal solution is selected; 2) measuring the light intensity of the inner wall of 304 stainless steel under two kinds of conditions: experimental group and control group, to verify whether the inner wall of 304 stainless steel can meet the demand of plant light source. In addition, ANSYS was used to analyze the structure of rotation shaft; 3) FLUENT software was used to analyze the fluid of two-dimensional and three-dimensional rotating bioreactor, and to compare the shear stress of hydroponic plants in two-dimensional and three-dimensional rotating bioreactor. The results showed that: 1. The relationship between the off-axis position and the fluid shear force is studied. It is concluded that the closer the distance from the axis to the axis, the smaller the peak value of shear force. After the prototype was finished, it was applied to a variety of plant (red duckweed, amaranth) for rotation cultivation experiment. The experimental results showed that the three-dimensional rotating bioreactor had a significant effect on the root growth of red duckweed. In addition, the experimental results of plant height of Amaranth showed that the plant height of seedlings was slightly higher than that of the control under the same cultivation conditions. The experimental results showed that the cultivation position X axis was the base rotation axis and the distance from the axis was smaller. This method also verified the shear force theory of FLUENT fluid analysis.
【学位授予单位】：福建农林大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：Q94-337

【相似文献】