皮层谷氨酸能和GABA能神经元可塑性与学习效率相关
发布时间：2018-11-03 16:01
【摘要】：目的:通过建立的联合式学习记忆模型小鼠,探究学习效率与桶状皮层中不同类型神经元可塑性之间的关系。方法:1.造模:实验动物主要选用出生20天后的C57Thy1-YFP/GAD67-GFP小鼠(以下简称小鼠),实验小鼠总共分为两组,第一组小鼠在以乙酸丁酯作为气味源刺激训练的同时,机械摆动小鼠右侧胡须,摆动频率为5Hz,持续时间为20秒,每隔2小时训练一次,每天训练5次,连续训练10天,训练期间,将每只小鼠放在家庭笼中饲养并观察情况。第二组不同于第一组小鼠的是在给予乙酸丁酯作为气味源嗅觉刺激的同时没有胡须摆动的刺激条件,而是在嗅觉条件完成一小时后再刺激胡须使其周期性摆动,此为第二组实验小鼠的一个训练方法。一天内反复实验五次,从而形成不配对的对照训练组。2.行为监测:在每天的训练完成后,拍摄并分析小鼠胡须摆动情况,拍摄时只给予小鼠乙酸丁酯的嗅觉刺激(嗅觉诱导的胡须摆动情况),随后用MATLAB软件进行运动追踪胡须摆动轨迹。依据胡须的摆动轨迹情况对小鼠学习效率进行划分,第一组小鼠产生高效学习组、低效学习组,第二组小鼠为不对称训练对照组。3.电生理实验:联合式训练第6天时对各组小鼠分别进行电生理实验,本实验主要对小鼠脑内barrel区的谷氨酸能神经元和GABA能神经元的编码能力和突触传递效率这两类指标的变化进行着重考察和分析。结果:1.行为学结果:(1)高效学习组与低效学习组相比,小鼠胡须摆动频率增加(P0.05),角度增加(P0.05);(2)低效学习组与不对称训练组,小鼠胡须摆动频率增加(P0.01),角度增加(P0.01);(3)高效学习组与不对称训练组,小鼠胡须摆动频率显著增加(P0.001),角度显著增加(P0.001)。2.电生理实验结果:(1)谷氨酸能神经元实验结果:1)高效学习组与低效学习组相比,小鼠大脑桶状皮层谷氨酸能神经元在单位时间内动作电位发放数量增加(P0.05),s EPSC(自发性兴奋性突触后电流)的电流发放间距减小(P0.05)、幅度增加(P0.05),s IPSC(自发性抑制性突触后电流)的电流发放间距增加(P0.05)、幅度减小(P0.05);2)低效学习组与不对称训练组相比,小鼠大脑桶状皮层的单位时间内动作电位发放个数增加(P0.051),s EPSC(自发性兴奋性突触后电流)的电流发放间距减小(P0.01)、幅度增加(P0.01),s IPSC(自发性抑制性突触后电流)的电流发放间距增加(P0.01)、幅度减小(P0.01);3)高效学习组与不对称训练组相比,小鼠大脑桶状皮层谷氨酸能神经元在单位时间内动作电位发放个数增加(P0.001),s EPSC(自发性兴奋性突触后电流)的发放间距减小(P0.001)、幅度增加(P0.001),s IPSC(自发性抑制性突触后电流)的发放间距增加(P0.001)、幅度减小(P0.001)。(2)GABA能神经元实验结果:1)高效学习组与低效学习组相比,小鼠大脑桶状皮层GABA能神经元在单位时间内动作电位发放个数减少(P0.05),s EPSC(自发性兴奋性突触后电流)的电流发放间距增加(P0.05)、幅度减小(P0.05);2)低效学习组与不对称训练组相比,小鼠大脑桶状皮层GABA能神经元在单位时间内动作电位发放个数减少(P0.01),s EPSC(自发性兴奋性突触后电流)的电流发放间距增加(P0.01)、幅度减小(P0.01);3)高效学习组与不对称训练组相比,小鼠大脑桶状皮层GABA能神经元在单位时间内动作电位发放个数减少(P0.001),s EPSC(自发性兴奋性突触后电流)的电流发放间距增加(P0.001)、幅度减小(P0.001)。结论:配对的胡须和气味刺激能够建立嗅觉诱导的胡须摆动情况,且出现高学习效率和低学习效率。学习效率与桶状皮层不同类型神经元可塑性变化相关。与未配对训练组相比,配对训练组的小鼠桶状皮层谷氨酸能神经元功能上调,GABA能神经元功能下调;且学习效率高的小鼠这种上调和下调比学习效率低的小鼠更显著。
[Abstract]:Objective: To explore the relationship between learning efficiency and plasticity of different types of neurons in barrel-shaped cortex by establishing a mouse model of combined learning and memory model. Method: 1. Model: The experimental animals were randomly divided into two groups: the first group of mice were randomly divided into two groups: the first group of mice stimulated the training with butyl acetate as the odor source, the right beards of the mice were shaken, the swing frequency was 5Hz, and the duration was 20 seconds. Each mouse was trained once every 2 hours, five times a day, 10 days of continuous training, and each mouse was kept and observed in a family cage during training. The second group is different from the first group of mice, which is a training method for the second group of experimental mice after the stimulation condition that butyl acetate is administered as an odor source olfactory stimulus without a beard swing, but is stimulated periodically after the olfactory condition is completed for one hour. Repeat the experiment five times a day to form an unpaired control training group. Behavior monitoring: After each day's training, shoot and analyze the rocking condition of the mouse's beard. Only the olfactory stimuli of butyl acetate (smell induced beard swing) were given during shooting, and then the trajectory of beard swing was traced with MATLAB software. The learning efficiency of the mice was divided on the basis of the swinging locus of the beard. The first group of mice produced the high-efficiency learning group and the inefficient learning group. The second group of mice was the asymmetric training control group. Electrophysiological experiment: Electrophysiological experiments were carried out on each group of mice on the sixth day of combined training. The experiment was mainly focused on the changes of the coding ability and synaptic transmission efficiency of glutamate neurons and GABA-energy neurons in the barrel region of mice. Result: 1. Results: (1) Compared with inefficient learning group, the swinging frequency of beard of mice increased (P 0.05), the angle increased (P 0.05), (2) inefficient learning group and asymmetric training group increased (P0.01), and the angle increased (P0.01). (3) The swinging frequency of beard of mice increased significantly (P0.001) and the angle was increased significantly (P0.001) in the high efficiency learning group and the asymmetric training group (P0.001). The results of electrophysiological experiments: (1) The experimental results of glutamate neurons: 1) Compared with inefficient learning group, the number of action potentials of glutamate neurons in barrel-shaped cortex of mice increased in unit time (P0.05). The current distribution interval of s EPSC (spontaneous excitability postsynaptic current) decreased (P 0.05), the current distribution interval of s IPSC (spontaneous inhibitory postsynaptic current) increased (P0.05), and the amplitude decreased (P0.05). The number of action potential distribution in the barrel cortex of mouse brain increased (P 0.01), and the current distribution interval of s EPSC (spontaneous excitability postsynaptic current) decreased (P0.01), and the amplitude was increased (P0.01). The current distribution interval of s IPSC (spontaneous inhibitory postsynaptic current) increased (P0.01), and the amplitude was decreased (P0.01); 3) The number of action potentials (P0.001) was increased in barrel-shaped cortical glutamate neurons in mouse brain compared with asymmetric training group (P <0.01). The distribution interval of s EPSC (spontaneous excitability postsynaptic current) decreased (P0. 001), the distribution interval of s IPSC (spontaneous inhibitory postsynaptic current) increased (P0.001), and the amplitude decreased (P0.001). (2) GABA-energy neuron experiment results: 1) Compared with inefficient learning group, GABA-energy neurons in barrel-shaped cortex of mice decreased the number of action potential distribution in unit time (P0.05), and the current distribution interval of s EPSC (spontaneous excitable synaptic current) increased (P0.05). Compared with asymmetric training group, GABA-energy neurons in barrel-shaped cortex of mice decreased the number of action potentials in unit time (P0.01), and the current distribution interval of s EPSC (spontaneous excitability postsynaptic current) increased (P0.01). Compared with asymmetric training group, GABA-energy neurons in barrel-shaped cortex of mice decreased the number of action potentials (P0.001) in unit time compared with asymmetric training group (P <0.01), and the current distribution interval of s EPSC (spontaneous excitable synaptic current) increased (P <0.001). Amplitude decreased (P0.001). Conclusion: Paired beard and odor stimulation can establish olfactory induced beard swing and high learning efficiency and low learning efficiency. The learning efficiency was related to the plasticity of different types of neurons in barrel cortex. Compared with the unpaired training group, the mouse barrel-shaped cortical glutamate neuron function of the paired training group is up-regulated, the function of GABA can be down-regulated, and the mouse with high learning efficiency is more remarkable than the mice with low learning efficiency.
【学位授予单位】：蚌埠医学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R338
本文编号：2308256
[Abstract]:Objective: To explore the relationship between learning efficiency and plasticity of different types of neurons in barrel-shaped cortex by establishing a mouse model of combined learning and memory model. Method: 1. Model: The experimental animals were randomly divided into two groups: the first group of mice were randomly divided into two groups: the first group of mice stimulated the training with butyl acetate as the odor source, the right beards of the mice were shaken, the swing frequency was 5Hz, and the duration was 20 seconds. Each mouse was trained once every 2 hours, five times a day, 10 days of continuous training, and each mouse was kept and observed in a family cage during training. The second group is different from the first group of mice, which is a training method for the second group of experimental mice after the stimulation condition that butyl acetate is administered as an odor source olfactory stimulus without a beard swing, but is stimulated periodically after the olfactory condition is completed for one hour. Repeat the experiment five times a day to form an unpaired control training group. Behavior monitoring: After each day's training, shoot and analyze the rocking condition of the mouse's beard. Only the olfactory stimuli of butyl acetate (smell induced beard swing) were given during shooting, and then the trajectory of beard swing was traced with MATLAB software. The learning efficiency of the mice was divided on the basis of the swinging locus of the beard. The first group of mice produced the high-efficiency learning group and the inefficient learning group. The second group of mice was the asymmetric training control group. Electrophysiological experiment: Electrophysiological experiments were carried out on each group of mice on the sixth day of combined training. The experiment was mainly focused on the changes of the coding ability and synaptic transmission efficiency of glutamate neurons and GABA-energy neurons in the barrel region of mice. Result: 1. Results: (1) Compared with inefficient learning group, the swinging frequency of beard of mice increased (P 0.05), the angle increased (P 0.05), (2) inefficient learning group and asymmetric training group increased (P0.01), and the angle increased (P0.01). (3) The swinging frequency of beard of mice increased significantly (P0.001) and the angle was increased significantly (P0.001) in the high efficiency learning group and the asymmetric training group (P0.001). The results of electrophysiological experiments: (1) The experimental results of glutamate neurons: 1) Compared with inefficient learning group, the number of action potentials of glutamate neurons in barrel-shaped cortex of mice increased in unit time (P0.05). The current distribution interval of s EPSC (spontaneous excitability postsynaptic current) decreased (P 0.05), the current distribution interval of s IPSC (spontaneous inhibitory postsynaptic current) increased (P0.05), and the amplitude decreased (P0.05). The number of action potential distribution in the barrel cortex of mouse brain increased (P 0.01), and the current distribution interval of s EPSC (spontaneous excitability postsynaptic current) decreased (P0.01), and the amplitude was increased (P0.01). The current distribution interval of s IPSC (spontaneous inhibitory postsynaptic current) increased (P0.01), and the amplitude was decreased (P0.01); 3) The number of action potentials (P0.001) was increased in barrel-shaped cortical glutamate neurons in mouse brain compared with asymmetric training group (P <0.01). The distribution interval of s EPSC (spontaneous excitability postsynaptic current) decreased (P0. 001), the distribution interval of s IPSC (spontaneous inhibitory postsynaptic current) increased (P0.001), and the amplitude decreased (P0.001). (2) GABA-energy neuron experiment results: 1) Compared with inefficient learning group, GABA-energy neurons in barrel-shaped cortex of mice decreased the number of action potential distribution in unit time (P0.05), and the current distribution interval of s EPSC (spontaneous excitable synaptic current) increased (P0.05). Compared with asymmetric training group, GABA-energy neurons in barrel-shaped cortex of mice decreased the number of action potentials in unit time (P0.01), and the current distribution interval of s EPSC (spontaneous excitability postsynaptic current) increased (P0.01). Compared with asymmetric training group, GABA-energy neurons in barrel-shaped cortex of mice decreased the number of action potentials (P0.001) in unit time compared with asymmetric training group (P <0.01), and the current distribution interval of s EPSC (spontaneous excitable synaptic current) increased (P <0.001). Amplitude decreased (P0.001). Conclusion: Paired beard and odor stimulation can establish olfactory induced beard swing and high learning efficiency and low learning efficiency. The learning efficiency was related to the plasticity of different types of neurons in barrel cortex. Compared with the unpaired training group, the mouse barrel-shaped cortical glutamate neuron function of the paired training group is up-regulated, the function of GABA can be down-regulated, and the mouse with high learning efficiency is more remarkable than the mice with low learning efficiency.
【学位授予单位】：蚌埠医学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R338
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