‘蓓蕾’蓝靛果花色苷组成鉴定及其单体微胶囊稳定性的研究

发布时间：2018-08-12 10:17

【摘要】：本论文对人工培育的新品种-'蓓蕾'蓝靛果中的花色苷进行了提取纯化工艺优化研究,以最优工艺制备得到花色苷冻干粉样品,对其中花色苷总量、种类进行了检测鉴定,并比较分析其抗氧化能力。另外,试验对'蓓蕾'蓝靛果花色苷中含量最高的矢车菊-3-葡萄糖苷进行了单体制备。对矢车菊-3-葡萄糖苷进行了微胶囊包埋处理,以提高其稳定性,并对高矢车菊-3-葡萄糖苷微胶囊的稳定性进行了研究。为'蓓蕾'蓝靛果产品推广及矢车菊-3-葡萄糖苷单体应用提供数据支持。通过试验及数据分析,主要研究成果如下:(1)利用单因素试验研究提取剂种类、超声波助提时间、料液比、提取剂浓度对提取'蓓蕾'蓝靛果中花色苷效果的影响,之后采用正交试验及方差分析得到提取'蓓蕾'蓝靛果中的花色苷的最佳工艺参数为:用体积分数为60%经0.1%盐酸酸化的乙醇进行提取,料液比为1:10mg/mL,超声波助提时间90min。(2)在花色苷纯化研究中,发现AB-8、X-5、D101、XAD7、NKA-9五种大孔树脂中D101型大孔树脂的吸附、解析能力最佳,并通过试验确定了上样浓度1.Omg/mL、pH=3、吸附时间120min、60%的乙醇洗脱等重要工艺参数。在此条件下制得的'蓓蕾'蓝靛果花色苷粗提物中花色苷含量为353.35±0.79mg/g,纯度由4.76%提高到了 35.43%。(3)利用HPLC-MS法鉴定'蓓蕾'蓝靛果中的花色苷组成,共11种花色苷被发现,其中矢车菊-3-葡萄糖苷占花色苷总量90.679%,并且矢车菊-3-乙酰己糖苷(MS =491;MS2=287)首次在蓝靛果中发现。此外,试验还通过总抗氧化能力(T-AOC)测定和ABTS+ ·、DPPH自由基清除能力测定,将蓝靛果花色苷提取物、矢车菊-3-葡萄糖苷、VC的抗氧化能力进行比较分析。数据显示,以上3种物质的抗氧化能力排序为:矢车菊-3-葡萄糖苷花色苷提取物VC。(4)利用半制备液相色谱法制备出'蓓蕾'蓝靛果花色苷中含量最高的矢车菊-3-葡萄糖苷,其冻干粉纯度达到97.8%。正交试验优化得到制备矢车菊-3-葡萄糖苷微胶囊的工艺条件为芯材:壁材为1:97,大豆分离蛋白:麦芽糊精为2:8,均质时间为8min。在此条件下制得微胶囊效果良好,其包埋率为92.17 ±1.87%,微胶囊化率为78.42 ±1.23%,溶解度为 76.21 ± 2.14%,含水率为 5.98 ±0.5%。(5)研究发现矢车菊-3-葡萄糖对光照和高温非常敏感,空气对其影响较小,试验证明包埋处理能显著提高矢车菊-3-葡萄糖苷对光照、温度氧气及消化稳定性。
[Abstract]:In this paper, the extraction and purification of anthocyanin from a new breed of "Bud Bud" Lonicera indigo were studied. The samples of anthocyanin freeze-dried powder were prepared by the optimum technology, and the total amount and species of anthocyanin were detected and identified. The antioxidant capacity was compared and analyzed. In addition, the highest content of anthocyanin-3-glucoside of Lonicera edulis was prepared. In order to improve its stability, the microencapsulation of cornflower-3-glucoside was carried out in order to improve its stability, and the stability of high-cornflower-3-glucoside microcapsule was studied. It provides data support for the product promotion of Lonicera edulis and the application of the-3-glucoside monomer of cornflower-3-glucoside. The main results are as follows: (1) the effects of extraction agents, ultrasonic extraction time, ratio of material to liquid and concentration of extractant on the extraction of anthocyanin from 'Bud' Lonicera indigo fruit were studied by single factor test. The optimum technological parameters for extracting anthocyanin from 'Bud Bud' Lonicera edulis fruit were obtained by orthogonal test and variance analysis. The optimum technological parameters were as follows: 60% volume fraction was extracted by 0.1% hydrochloric acid acidified ethanol. The ratio of solid to liquid was 1: 10 mg / mL, and the extraction time was 90 min. (2) in the study of anthocyanin purification, it was found that the adsorption of D101 macroporous resin was the best. Some important technological parameters, such as pH ~ (3), adsorption time of 120 min ~ (60%) and ethanol elution, were determined. Under these conditions, the anthocyanin content in the crude extract of 'Bud' Lonicera edulis was 353.35 卤0.79 mg / g, and the purity was increased from 4.76% to 35.43%. (3) the anthocyanin composition of 'Bud'Lonicera indigo was identified by HPLC-MS, and 11 anthocyanins were found. Among them, the total amount of anthocyanin was 90.679 9%, and the first time was found in Lonicera edulis fruit. In addition, the total antioxidant capacity (T-AOC) and the free radical scavenging ability of ABTS were measured to compare and analyze the antioxidant ability of anthocyanin extract of Lonicera edulis and the VC of Corydanthemum 3-glucoside. The results showed that the order of antioxidant ability of the above three substances was as follows: (4) the highest content of the anthocyanin of 'Bud'Lonicera indigo was prepared by semi-prepared liquid chromatography. The purity of the freeze-dried powder is 97.8%. The optimum conditions for preparation of cornflower -3-glucoside microcapsules were as follows: core material: wall material 1: 97, soy protein isolate: maltodextrin 2: 8, homogenization time 8 min. The results showed that the encapsulation rate was 92.17 卤1.87, the encapsulation rate was 78.42 卤1.23, the solubility was 76.21 卤2.14 and the moisture content was 5.98 卤0.5. (5) the results showed that cornflower -3- glucose was sensitive to light and high temperature, but air had little effect on it. The results showed that the embedding treatment could significantly improve the stability of sunlight, temperature, oxygen and digestion of cornflower-3-glucoside.
【学位授予单位】：沈阳农业大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：TS264.4

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