下丘脑神经肽（NPY、AgRP、POMC、CART）在大绒鼠体重和能量代谢中调节作用的研究
发布时间：2019-05-15 18:55
【摘要】：大绒鼠(Eothenomys miletus)为中国特有种,主要分布于横断山区及其附近地区,为横断山区固有种。之前的研究结果表明瘦素在不同条件下对于大绒鼠的体重和能量代谢调节是不一样的,但是神经机理调节还不是很清楚,为了进一步了解瘦素在大绒鼠的体重和能量代谢调节机理,本论文从下丘脑神经肽基因进一步研究瘦素是如何调节的,实验包括季节性驯化,温度、光照和食物的单因素实验以及三个因子的三因素实验,最终来探讨下丘脑神经肽基因表达量对大绒鼠能量稳态的调节作用,以确定大绒鼠在横断山区的适应模式,并为进一步研究下丘脑神经元在小型哺乳动物中的体重调节和能量稳态调节奠定了基础。实验结果分为五个部分：1、下丘脑神经肽基因表达量对大绒鼠体重和能量代谢季节性变化的影响：大绒鼠的体重和体脂均出现了季节性变化,冬季较高,夏季较低,食物摄入量季节性差异显著,在冬季较高,夏季最低。血清瘦素含量也出现了季节性变化,和体脂变化趋势类似,瘦素含量与脂肪含量呈正相关关系。下丘脑神经肽基因NPY和AgRP表达量季节性差异显著,而POMC和CART表达量差异不显著。瘦素与NPY和AgRP负相关,与POMC和CART正相关。以上结果表明大绒鼠在季节性变化过程中冬季降低体重、体脂,增加摄入量来维持生存。瘦素通过作用于下丘脑神经肽基因调节大绒鼠的体重和能量代谢。2、下丘脑神经肽基因表达量在冷驯化条件下对大绒鼠体重和能量代谢的影响：低温降低大绒鼠的体重和体脂,增加摄入量。对照组和低温组的瘦素含量差异显著,瘦素含量与脂肪含量呈正相关关系。下丘脑神经肽基因NPY表达量差异显著,而AgRP、POMC和CART表达量差异不显著。瘦素与NPY负相关,与CART正相关,与POMC和AgRP不相关。以上结果表明低温可以降低大绒鼠的体重、体脂和瘦素含量,增加摄入量和NPY表达量。瘦素通过调节NPY和CART表达量来调节大绒鼠的体重和能量代谢。3、下丘脑神经肽基因表达量在不同光照条件下对大绒鼠体重和能量代谢的影响：短光降低大绒鼠的体重和体脂,增加摄入量。光照不影响瘦素含量,瘦素含量与脂肪含量呈正相关关系。下丘脑神经肽基因NPY、AgRP、POMC和CART表达量差异不显著。瘦素与NPY负相关,与POMC、AgRP和CART不相关。以上结果表明短光可以降低大绒鼠的体重和体脂,增加摄入量。瘦素通过调节NPY表达量来调节大绒鼠的体重和能量代谢。4、下丘脑神经肽基因表达量在限食条件下对大绒鼠体重和能量代谢的影响：限食降低大绒鼠的体重和体脂,增加摄入量。对照组和限食组的瘦素含量差异显著,瘦素含量与脂肪含量呈正相关关系。下丘脑神经肽基因NPY、AgRP、POMC和CART表达量差异显著。瘦素与NPY和AgRP负相关,与POMC和CART正相关。以上结果表明限食可以降低大绒鼠的体重、体脂和瘦素含量,增加摄入量和下丘脑神经肽基因表达量。瘦素通过调节NPY/AgRP和POMC/CART表达量来调节大绒鼠的体重和能量代谢。5、温度、光照和食物对大绒鼠体重和下丘脑神经肽基因表达量的影响：低温和限食可以降低体重和体脂,瘦素含量也显著降低,NPY和AgRP表达量显著增加,而POMC和CART表达量显著降低,而光照对于以上指标影响不显著。瘦素与体脂、POMC和CART表达量正相关,与NPY和AgRP表达量负相关。以上结果表明温度和食物是影响大绒鼠体重和能量代谢的因素,瘦素在该环境下通过调节下丘脑神经肽基因的表达量来调节大绒鼠的能量稳态。综上所述：大绒鼠在季节性变化过程中通过调整下丘脑神经肽基因表达量来调节体重和能量代谢,具体表现在：低温条件下主要通过调节NPY和CART表达量、短光可以刺激NPY表达量增加,限食可以改变NPY/AgRP和POMC/CART表达量。对于环境因子影响作用的大小,低温和限食使NPY和AgRP表达量显著增加,而POMC和CART表达量显著降低,而光照对于以上指标影响不显著。大绒鼠在季节变化和不同环境因子条件下的下丘脑神经肽基因表达量的变化最终来适应横断山区年温差小,日温差大,食物资源相对丰富的栖息环境。
[Abstract]:Eothrenomys miletus is a kind of endemic species in China, which is mainly distributed in Hengdushan District and its adjacent area, and is an inherent species of Hengdushan District. Previous studies have shown that leptin is different under different conditions for the body weight and energy metabolism of the large-wool rats, but the regulation of the nerve mechanism is not clear, in order to further understand the mechanism of the adjustment of the weight and energy metabolism of leptin in the rats, This paper further studies how leptin is regulated from the hypothalamic neuropeptide gene, including the single factor experiment of seasonal acclimatization, temperature, light and food, and the three factor experiment of three factors. Finally, the regulation of the expression of neuropeptide gene in the hypothalamus on the steady state of the energy of the large-wool mice was discussed to determine the adaptive pattern of the large-pile mice in the Hengduan area and lay the foundation for further study of the weight regulation and the steady-state regulation of the hypothalamic neurons in the small mammals. The results of the experiment were divided into five parts:1. The effect of the expression of the hypothalamic neuropeptide gene on the body weight and the seasonal change of energy metabolism: the body weight and body fat of the rats were seasonal, the winter was higher, the summer was lower, and the seasonal difference of the food intake was significant. In winter, the lowest in summer. The serum leptin content also has a seasonal change, and the change of the body fat is similar, and the leptin content is positively related to the fat content. There was a significant seasonal difference in the expression of NPY and AgP in the hypothalamic neuropeptide gene, while the difference between the expression of POMC and CART was not significant. Leptin was negatively correlated with NPY and AgRP, and was positively correlated with POMC and CART. The results showed that in the course of seasonal variation, the rats were able to reduce body weight, body fat and increase the intake to maintain the survival. The effect of leptin on the body weight and energy metabolism of the large-wool rats was regulated by the action of the neuropeptide gene in the hypothalamus. The effect of the expression of the neuropeptide gene on the body weight and energy metabolism of the rats under cold acclimatization was studied. The leptin content in the control group and the low-temperature group was significant, and the leptin content was positively related to the fat content. There was a significant difference in the expression of NPY in the hypothalamic neuropeptide gene, and the difference of the expression of agRP, POMC and CART was not significant. Leptin is negatively associated with NPY and is not associated with CART and is not associated with POMC and AgRP. The results showed that low temperature could reduce the body weight, body fat and leptin content, increase the intake and NPY expression. Leptin regulates the weight and energy metabolism of the rats by regulating the expression of NPY and CART.3. The effect of the expression of the neuropeptide gene in the hypothalamus on the body weight and energy metabolism of the rats under different light conditions: the short light reduces the body weight and body fat of the large-wool and increases the intake. The light does not affect the leptin content, and the leptin content is positively related to the fat content. The expression of NPY, AgRP, POMC and CART in the hypothalamic neuropeptide gene was not significant. Leptin is negatively related to NPY and is not related to POMC, AgRP and CART. The above results show that the short light can lower the body weight and body fat and increase the intake. Leptin regulates the weight and energy metabolism of the rats by regulating the expression of NPY. The leptin content in the control group and the limited group was significant, and the leptin content was positively related to the fat content. The expression of NPY, AgRP, POMC and CART in the hypothalamic neuropeptide gene was significant. Leptin was negatively correlated with NPY and AgRP, and was positively correlated with POMC and CART. The above results show that the limited food can reduce the body weight, body fat and leptin content, increase the intake and the expression of the neuropeptide gene in the hypothalamus. Leptin regulates the weight and energy metabolism of the rats by adjusting the expression of NPY/ AgRP and POMC/ CART. The effects of 5, temperature, light and food on the expression of neuropeptide gene in the body weight and the hypothalamus of the female rats: the low temperature and the limited food can reduce the body weight and body fat, and the leptin content is also significantly reduced. The expression of NPY and AgRP was significantly increased, while the expression of POMC and CART was significantly decreased, and the effect of light on the above indexes was not significant. Leptin was positively correlated with the expression of body fat, POMC and CART, and negatively correlated with the expression of NPY and AgRP. The above results show that the temperature and the food are the factors that affect the body weight and energy metabolism of the rats, and the leptin can regulate the energy homeostasis of the rats by adjusting the expression of the hypothalamic neuropeptide gene in the environment. To sum up, in the course of seasonal variation, the large-pile mouse adjusts the weight and energy metabolism by adjusting the expression amount of the neuropeptide gene in the hypothalamus, which is manifested in that the NPY and the CART expression amount are mainly regulated under the low-temperature condition, and the short light can stimulate the increase of the expression amount of the NPY, The restriction can change the expression of NPY/ AgRP and POMC/ CART. For the effects of environmental factors, the expression of NPY and AgRP was increased significantly, while the expression of POMC and CART was significantly decreased, and the effects of light on the above indexes were not significant. The change of the expression of the hypothalamic neuropeptide gene under the conditions of seasonal variation and different environmental factors is the last to adapt to the small temperature difference, the large temperature difference and the relative abundance of food resources in the Hengdushan region.
【学位授予单位】：南京师范大学
【学位级别】：博士
【学位授予年份】：2015
【分类号】：Q493
本文编号：2477727
[Abstract]:Eothrenomys miletus is a kind of endemic species in China, which is mainly distributed in Hengdushan District and its adjacent area, and is an inherent species of Hengdushan District. Previous studies have shown that leptin is different under different conditions for the body weight and energy metabolism of the large-wool rats, but the regulation of the nerve mechanism is not clear, in order to further understand the mechanism of the adjustment of the weight and energy metabolism of leptin in the rats, This paper further studies how leptin is regulated from the hypothalamic neuropeptide gene, including the single factor experiment of seasonal acclimatization, temperature, light and food, and the three factor experiment of three factors. Finally, the regulation of the expression of neuropeptide gene in the hypothalamus on the steady state of the energy of the large-wool mice was discussed to determine the adaptive pattern of the large-pile mice in the Hengduan area and lay the foundation for further study of the weight regulation and the steady-state regulation of the hypothalamic neurons in the small mammals. The results of the experiment were divided into five parts:1. The effect of the expression of the hypothalamic neuropeptide gene on the body weight and the seasonal change of energy metabolism: the body weight and body fat of the rats were seasonal, the winter was higher, the summer was lower, and the seasonal difference of the food intake was significant. In winter, the lowest in summer. The serum leptin content also has a seasonal change, and the change of the body fat is similar, and the leptin content is positively related to the fat content. There was a significant seasonal difference in the expression of NPY and AgP in the hypothalamic neuropeptide gene, while the difference between the expression of POMC and CART was not significant. Leptin was negatively correlated with NPY and AgRP, and was positively correlated with POMC and CART. The results showed that in the course of seasonal variation, the rats were able to reduce body weight, body fat and increase the intake to maintain the survival. The effect of leptin on the body weight and energy metabolism of the large-wool rats was regulated by the action of the neuropeptide gene in the hypothalamus. The effect of the expression of the neuropeptide gene on the body weight and energy metabolism of the rats under cold acclimatization was studied. The leptin content in the control group and the low-temperature group was significant, and the leptin content was positively related to the fat content. There was a significant difference in the expression of NPY in the hypothalamic neuropeptide gene, and the difference of the expression of agRP, POMC and CART was not significant. Leptin is negatively associated with NPY and is not associated with CART and is not associated with POMC and AgRP. The results showed that low temperature could reduce the body weight, body fat and leptin content, increase the intake and NPY expression. Leptin regulates the weight and energy metabolism of the rats by regulating the expression of NPY and CART.3. The effect of the expression of the neuropeptide gene in the hypothalamus on the body weight and energy metabolism of the rats under different light conditions: the short light reduces the body weight and body fat of the large-wool and increases the intake. The light does not affect the leptin content, and the leptin content is positively related to the fat content. The expression of NPY, AgRP, POMC and CART in the hypothalamic neuropeptide gene was not significant. Leptin is negatively related to NPY and is not related to POMC, AgRP and CART. The above results show that the short light can lower the body weight and body fat and increase the intake. Leptin regulates the weight and energy metabolism of the rats by regulating the expression of NPY. The leptin content in the control group and the limited group was significant, and the leptin content was positively related to the fat content. The expression of NPY, AgRP, POMC and CART in the hypothalamic neuropeptide gene was significant. Leptin was negatively correlated with NPY and AgRP, and was positively correlated with POMC and CART. The above results show that the limited food can reduce the body weight, body fat and leptin content, increase the intake and the expression of the neuropeptide gene in the hypothalamus. Leptin regulates the weight and energy metabolism of the rats by adjusting the expression of NPY/ AgRP and POMC/ CART. The effects of 5, temperature, light and food on the expression of neuropeptide gene in the body weight and the hypothalamus of the female rats: the low temperature and the limited food can reduce the body weight and body fat, and the leptin content is also significantly reduced. The expression of NPY and AgRP was significantly increased, while the expression of POMC and CART was significantly decreased, and the effect of light on the above indexes was not significant. Leptin was positively correlated with the expression of body fat, POMC and CART, and negatively correlated with the expression of NPY and AgRP. The above results show that the temperature and the food are the factors that affect the body weight and energy metabolism of the rats, and the leptin can regulate the energy homeostasis of the rats by adjusting the expression of the hypothalamic neuropeptide gene in the environment. To sum up, in the course of seasonal variation, the large-pile mouse adjusts the weight and energy metabolism by adjusting the expression amount of the neuropeptide gene in the hypothalamus, which is manifested in that the NPY and the CART expression amount are mainly regulated under the low-temperature condition, and the short light can stimulate the increase of the expression amount of the NPY, The restriction can change the expression of NPY/ AgRP and POMC/ CART. For the effects of environmental factors, the expression of NPY and AgRP was increased significantly, while the expression of POMC and CART was significantly decreased, and the effects of light on the above indexes were not significant. The change of the expression of the hypothalamic neuropeptide gene under the conditions of seasonal variation and different environmental factors is the last to adapt to the small temperature difference, the large temperature difference and the relative abundance of food resources in the Hengdushan region.
【学位授予单位】：南京师范大学
【学位级别】：博士
【学位授予年份】：2015
【分类号】：Q493
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