3D打印组织工程骨支架的骨改建机制研究

发布时间：2017-12-27 02:28

本文关键词：3D打印组织工程骨支架的骨改建机制研究　出处：《新疆医科大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的:观察由双向陶瓷骨粉(DCCP)与聚乙烯醇(PVA)凝胶混合构建的3D打印组织工程骨支架支架,检测其微观结构及理化性能,同时将材料回植入实验动物体内,通过扫面电镜、实时定量PCR等方法检测相关成骨基因Collagen-I、OC、OPN、破骨基因CSF-1、TNF、IL-1等在回植后体内的表达变化,研究其骨改建的机制。方法:通过3D打印成形技术制作双相陶瓷化骨粉/PVA组织工程骨支架与纳米羟基磷灰石(NHA)/PVA组织工程骨支架,检测对比支架的微观结构、孔隙率、吸水率、压缩性能、细胞毒性,同时将3D打印双相陶瓷化骨粉/PVA组织工程骨支架回植入实验动物颌骨内,采用同体同颌对照,将实验动物分为三组,通过扫面电镜、HE染色、实时定量PCR的方法,研究该支架在体内的骨改建机制。结果:(1)3D打印的双相陶瓷化骨粉/PVA组织工程骨支架孔径为240-600μm;孔隙率为49.43±8.21%;吸水性为24小时试点为23.84±0.74%;力学性能200Mpa时应变为25%;培养7d后,双相陶瓷化骨粉/PVA组织工程骨支架表面活细胞数量高于对照组(P0.05)(2)3D打印的双相陶瓷化骨粉/PVA支架在回植动物体内后,OC、OPN、Collagen-I、CSF-1、TNF-α与OPG的表达量在三月试点即骨改建中后期结束时,均显示实验组大于对照组(P0.05);IL-1显示对照组大于实验组。结论:3D打印制备的双相陶瓷化骨粉/PVA组织工程骨支架在回植后不同时段各基因表达差异明显,其中其在骨组织修复过程中的表达量可猜测与材料降解速率相关,可以证明在骨组织修复过程中,该支架可以诱导周围骨组织快速修复,增强骨缺损的骨改建愈合速度。
[Abstract]:Objective: To observe the bidirectional ceramic bone (DCCP) and polyvinyl alcohol (PVA) gel mixed construction of 3D printing scaffolds in tissue engineering scaffolds, detection and physicochemical properties of the micro structure, and the material back to the in vivo experimental animal, scanning electron microscope and detected by real-time PCR method related to osteogenic gene Collagen-I, OC OPN, CSF-1, TNF, osteoclast gene and IL-1 expression in the back body after planting, to investigate the mechanism of bone remodeling. Methods: using 3D printing technology production of dual phase ceramic bovine bone /PVA bone tissue engineering scaffold with nano hydroxyapatite (NHA) /PVA scaffolds for bone tissue engineering, scaffold for contrast detection the microstructure, porosity, water absorption, compressive properties, cytotoxicity, and print 3D dual phase ceramic bovine bone /PVA bone tissue engineering scaffold implanted back the experimental animal bones, the body with the jaw control, experimental animal were divided into three groups by scanning electron microscopy, HE staining, real-time quantitative PCR method, study the remodeling mechanism of in vivo bone scaffold. Results: (1) dual phase ceramic 3D printing of bone tissue engineering bone scaffold /PVA diameter is 240-600 m; the porosity was 49.43 + 8.21%; water for 24 hour pilot was 23.84 + 0.74%; the mechanical properties of the 200Mpa strain was 25%; after 7d, the number of live cells in biphasic ceramic bovine bone tissue scaffold /PVA engineering bone surface is higher than that of control group (P0.05) (2) dual phase ceramic 3D printing of thebbp /PVA stent in replantation of animal body, expression of OC, OPN, Collagen-I, CSF-1, TNF- alpha and OPG in March that the pilot later remodeling at the end were showed in the experimental group than the control group (P0.05 IL-1); control group showed greater than the experimental group. Conclusion: 3D printing preparation of biphasic ceramic bone /PVA bone tissue engineering scaffold after implantation in different periods of each gene expression differences, including its expression in bone tissue repair process in the amount of biodegradable materials and guess rate can be demonstrated in the repair process, the bone, the scaffold can induce rapid repair of bone the surrounding tissue, enhance bone defect healing of bone remodeling.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R318.08

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