基于大数据分析的KRT1、KRT10、GSTP1在食管鳞癌中的表达及意义

发布时间：2017-12-28 03:01

本文关键词：基于大数据分析的KRT1、KRT10、GSTP1在食管鳞癌中的表达及意义　出处：《新疆医科大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的:分析KRT1、KRT10、GSTP1基因在各肿瘤中表达的大数据信息及在哈萨克族食管鳞癌组织中的表达及临床意义。方法:通过www.proteinatlas.org生物学信息公众平台,查询KRT1、KRT10、GSTP1基因在各正常人体组织及各肿瘤中的表达情况;运用Western blot技术对28对新鲜食管癌组织及相应的癌旁正常组织中的KRT1、KRT10、GSTP1表达情况进行半定量分析;用免疫组织化学技术(IHC)对60对食管鳞状细胞癌组织及癌旁正常食管黏膜组织中的KRT1、KRT10、GSTP1表达情况进行定性检测,结合临床病理资料,对免疫组化结果进行数据分析,并用ROC曲线对三个蛋白的诊断价值进行评估。结果:1.Western blot结果显示KRT1、KRT10、GSTP1三个蛋白在食管鳞状细胞癌组织中表达高于相应癌旁正常组织(P0.001),在哈萨克族与汉族中表达无统计学差异(P0.05);2.免疫组化结果显示KRT1、KRT10、GSTP1在食管鳞状细胞癌组织中的表达均高于癌旁正常组织(P0.001),结合临床病理参数分析,结果显示KRT1、KRT10的表达与食管鳞癌组织的分化程度有关(P0.05),GSTP1在食管鳞状细胞癌中的强阳性表达与患者的年龄、性别、族别、淋巴结转移与否有关(P0.05),根据ROC曲线下面积提示三个差异蛋白中KRT10蛋白的诊断价值最高,GSTP1蛋白其次,KRT1蛋白最低,三者均有诊断价值。结论:KRT1、KRT10、GSTP1为食管鳞状细胞癌的发生发展中的重要变化分子,KRT1、KRT10与食管鳞癌的分化有关,可作为食管鳞癌恶性程度判断的重要指标,KRT10有望成为食管鳞癌诊断中的特异性分子标记物,GSTP1与患者的年龄、性别、族别、淋巴结转移有关,可对食管鳞癌的协助诊断、恶性程度判断及后期个体化治疗有一定指导意义。
[Abstract]:Objective: to analyze the large data information expressed by KRT1, KRT10 and GSTP1 genes in various tumors and the expression and clinical significance in Kazak's esophageal squamous cell carcinoma. Methods: the www.proteinatlas.org biological information public platform, the query expression of KRT1, KRT10, GSTP1 gene in normal human tissues and tumor; using semi quantitative analysis of 28 pairs of cancer and corresponding fresh esophageal cancer tissues, the expression of GSTP1 Western KRT1, KRT10 blot; immunohistochemistry Chemical Technology (IHC) expression were detected in 60 of esophageal squamous cell carcinoma tissue and normal esophageal mucosa tissues of KRT1, KRT10, GSTP1, combined with clinical data, data analysis on the results of immunohistochemistry, and were evaluated by ROC curve in diagnosis of three proteins. Results: 1.Western blot showed that KRT1, KRT10 and GSTP1 three protein expression in esophageal squamous cell carcinoma tissues than adjacent normal tissues (P0.001), there were no significant difference in Kazak and Han in 2. (P0.05); immunohistochemistry showed that the expression of KRT1, KRT10 and GSTP1 in esophageal squamous cell carcinoma were higher than those in adjacent normal tissues (P0.001), combined with analysis of clinical pathological parameters, the results showed that KRT1, KRT10 expression and differentiation of esophageal squamous cell carcinoma (P0.05), GSTP1 strongly positive in esophageal squamous cell carcinoma and patients' age, gender, nationality, is associated with lymph node metastasis (P0.05), according to the diagnostic value of the area under the ROC curve showed that KRT10 protein three proteins in the highest GSTP1 protein KRT1 protein, secondly, the lowest value of three has diagnosis. Conclusion: KRT1, KRT10, GSTP1 as an important change in the development of molecular differentiation of esophageal squamous cell carcinoma, KRT1, KRT10 and esophageal squamous cell carcinoma, as an important index to judge the malignant degree of esophageal squamous cell carcinoma, KRT10 is expected to become the specific molecular markers for diagnosis of esophageal squamous cell carcinoma, GSTP1 and the age and sex of the patients, nationality and lymph node metastasis of esophageal squamous cell carcinoma, can assist diagnosis and individualized treatment of judging malignant degree and later have a certain significance.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R735.1

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