新疆维吾尔族、汉族脑梗死患者MTHFR基因多态性的差异研究

发布时间：2017-12-28 09:12

本文关键词：新疆维吾尔族、汉族脑梗死患者MTHFR基因多态性的差异研究　出处：《新疆医科大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的:比较新疆地域维吾尔族与汉族急性脑梗死病患的5,10-亚甲基四氢叶酸还原酶遗传因子677位点的单核苷酸遗传的差异性。方法:选取于新医大第一附属医院脑病科病房住院的维吾尔族(50例)、汉族(111例)急性脑梗死病人,取其外周血液,提取DNA,采用扩增-聚合酶链反应-芯片杂交法来检测5,10-亚甲基四氢叶酸还原酶遗传因子677位点单核苷酸突变特点,分析比较此遗传因子型在维族、汉族两民族急性脑梗死病患间的差别。结果:1)新疆地域的维吾尔族、汉族两民族急性脑梗死病人5,10-亚甲基四氢叶酸还原酶遗传因子677位点类型分布频率依次:TT型[38%(19/50)]比[19.8%(22/111)];CT型[36.0%(18/50)]比[28.83%(32/111)];CC型[26%(13/50)]比[51.37%(57/111)];T等位基因分布频率依次:56%比34.23%,维吾尔族急性脑梗死患者TT型及T等位基因频率明显高于汉族患者(P0.05)。2)维吾尔族、汉族急性脑梗死患者的5,10-亚甲基四氢叶酸还原酶遗传因子677位点3型相对应的同型半胱氨酸有显著差别(F=12.30,P=0.00),TT型明显高于CT型;CT型高于CC型,维吾尔族急性脑卒中病人的同型半胱氨酸浓度显著高于汉族病人(P=0.004)。3)维吾尔族、汉族两民族急性脑梗死病患在年龄、性别、收缩压值、血糖、低密度脂蛋白、抽烟史、喝酒史、体重指数方面差异均无统计差别(P0.05)。结论:5,10-亚甲基四氢叶酸还原酶遗传因子677位点出现T变异,而导致高同型半胱氨酸血症;TT基因型及T等位基因分布频率可能会增加脑梗死的患病风险,这种风险在新疆地区维吾尔族和汉族急性脑梗死人群之间存在统计学上的差异。但由于此研究纳入统计样本含量偏少,需扩大样本含量后,研究分析证实。
[Abstract]:Objective: To compare the genetic differences of 5,10- methylenetetrahydrofolate reductase gene locus 677 in Xinjiang area between Uygur and Han patients with acute cerebral infarction. Methods: the Uygur in encephalopathy Department of the First Affiliated Hospital of Xinjiang Medical University Hospital Ward (50 cases) and Han (111 cases) of patients with acute cerebral infarction, from peripheral blood, extracted DNA, to detect 5,10- methylenetetrahydrofolate reductase genetic factor 677 single nucleotide mutations were amplified with polymerase chain reaction chip hybridization analysis. Comparison of the genetic factors in Uygur and Han nationality type two acute cerebral infarction patients between. Results: 1 patients of Uygur and Han nationality) of two acute cerebral infarction 5,10- methylenetetrahydrofolate reductase genetic factors in Xinjiang region 677 site type distribution frequencies are: TT [38% (19/50)] [19.8% (22/111)]; CT [36.0% (18/50)] [28.83% (32/111)]; CC [26% (13/50)] than [51.37% (57/111)]; T allele frequencies are: 56% to 34.23%, Uygur patients with acute cerebral infarction TT genotype and T allele frequency was significantly higher than that in Han patients (P0.05). 2) there was significant difference between homocysteine 5,10- methylenetetrahydrofolate reductase genetic factors in patients with acute cerebral infarction in Han and Uygur type 3 corresponding to the 677 loci (F=12.30, P=0.00), TT was significantly higher than that of CT type; CT type is higher than that of the CC, homocysteine concentration disease Uygur acute stroke was significantly higher than that in Han patients (P=0.004). 3) there was no significant difference in age, sex, systolic blood pressure, blood sugar, low density lipoprotein, smoking history, drinking history and body mass index between two nationalities of Uygur and Han Nationalities (P0.05). Conclusion: 5,10- methylenetetrahydrofolate reductase genetic factor 677 loci of T mutation, resulting in hyperhomocysteinemia; TT genotype and T allele frequency distribution may increase the risk of cerebral infarction, there is a statistically significant difference between the Uygur and Han nationality in Xinjiang area, the risk of acute cerebral infarction group. However, due to the lack of statistical samples in this study, it is proved that the sample content should be expanded.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R743.33

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