急性冠脉综合征患者外周血红细胞源微粒水平的研究

发布时间：2017-12-31 11:33

本文关键词：急性冠脉综合征患者外周血红细胞源微粒水平的研究　出处：《新疆医科大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的:分析急性冠脉综合征(Acute Coronary Syndrome ACS)患者外周血红细胞源微粒的水平以及三种亚型之间的差异,评价外周血红细胞源微粒与ACS发生之间的关联,进一步评价外周血红细胞源微粒对于ACS早期诊断的临床意义。方法:研究对象分为Non-CAD组(n=45)和ACS组(n=105),其中ACS组分为急性ST段抬高性心梗组STEMI(n=37)、非ST段抬高性心梗组Non-STEMI(n=31)和不稳定型心绞痛组UAP(n=37),收集研究对象外周静脉血,离心后加入钙离子载体A23187,得到微粒。用特异性外周血红细胞源微粒抗体(glycophorine A CD235a)标记红细胞源微粒,经流式细胞仪进行定性定量分析。结果:ACS组外周血中红细胞源微粒水平为26.20[15.90,38.00],非冠心病组红细胞源微粒水平14.00[4.35,36.35],ACS组高于非冠心病组(P=0.005,P0.05),其中ACS组三种亚型的外周血红细胞源微粒水平STEMI 27.20[17.25,24.25],Non-STEMI21.50[12.76,34.90],UAP 30.20[17.10,39.65],ACS组各亚组之间红细胞源微粒水平的差异并无统计学意义(P0.05)。结论:急性冠脉综合征患者外周血红细胞源微粒水平高于正常对照组,提示外周血红细胞源微粒可能参与急性冠脉综合征的发生发展过程,可能与ACS发生急性心血管事件相关。
[Abstract]:Objective: to analyze the acute coronary syndrome (Acute Coronary Syndrome ACS) the difference between the levels of red blood cell particulates in patients with peripheral and three subtypes, evaluate the relationship between peripheral blood cells and ACS particle source, further evaluation of peripheral blood red cell source particles on clinical significance to the early diagnosis of ACS. Methods: the research objects were divided into Non-CAD group (n=45) and ACS group (n=105), the ACS group was divided into acute ST segment elevation myocardial infarction group (STEMI n=37), non ST segment elevation myocardial infarction group (Non-STEMI n=31) and unstable angina group (n=37 UAP), collected peripheral venous blood. After centrifugation, adding calcium ionophore A23187 obtained particles. With specific peripheral blood erythrocyte antibody (glycophorine A particulates CD235a) labeled red blood cell source particles by flow cytometry for qualitative and quantitative analysis. Results: the red blood cell ACS in peripheral blood level of 26. particles 20[15.90,38.00], non CHD group erythrocyte derived microparticles in 14.00[4.35,36.35], ACS group was higher than that of non CHD group (P=0.005, P0.05), group ACS included three subtypes of peripheral red blood cell source level of STEMI Non-STEMI21.50[12.76,34.90], 27.20[17.25,24.25] particles, UAP 30.20[17.10,39.65], ACS group between erythrocyte derived particle levels no significant difference (P0.05). Conclusion: the acute coronary syndrome in patients with peripheral blood level of particulate cell source higher than the normal control group, suggesting that the peripheral blood red cell particulates may be involved in the occurrence and development of acute coronary syndrome combined, may be associated with ACS occurrence of acute cardiovascular events.

【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R541.4

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