中药成分绿原酸和异绿原酸的敏化荧光与分析方法研究

发布时间：2018-01-01 10:12

本文关键词：中药成分绿原酸和异绿原酸的敏化荧光与分析方法研究　出处：《河北师范大学》2017年硕士论文　论文类型：学位论文

【摘要】：绿原酸和异绿原酸是重要的中药活性成分,广泛存在于药用植物中,具有多种药理作用。测定中药样品中绿原酸和异绿原酸的含量对于中药质量评价具有重要意义。本文研究了绿原酸和三种异绿原酸(包括异绿原酸A、异绿原酸B和异绿原酸C)的敏化荧光性质,进而研究了菊花和金银花中绿原酸和异绿原酸的荧光分析方法,并用薄层吸光扫描法分别测定了金银花中绿原酸、异绿原酸A和异绿原酸C的含量。论文包括以下三部分:1.研究了绿原酸和三种异绿原酸的荧光性质。绿原酸和异绿原酸水溶液的内源荧光均很弱,加入硼砂和溴化十六烷基三甲胺(CTAB)可产生较强的敏化荧光。绿原酸荧光峰的最大激发/发射波长位于350 nm/460 nm,异绿原酸A在350 nm/480 nm,异绿原酸B在350 nm/471nm,异绿原酸C在350 nm/480 nm。四种物质的敏化荧光强度均与水溶液中甲醇的体积分数、离子强度、硼砂和CTAB的浓度有关。在最佳实验条件下,测得绿原酸的荧光量子产率为0.0177,异绿原酸A为0.0167,异绿原酸B为0.0154,异绿原酸C为0.0248。2.由于绿原酸和异绿原酸的荧光性质非常相似,在不经分离的情况下难以测定各种组分含量,因而用荧光分光光度法和三维荧光二阶校正法测定了菊花和金银花中绿原酸和异绿原酸的总量。两种方法对菊花的测定结果的平均值分别为5.59%和5.40%,对金银花测定结果的平均值分别为7.05%和8.48%。研究结果表明,荧光分析法可以测定菊花和金银花中绿原酸和异绿原酸的总量,进而可用于评估药材的质量。3.用薄层吸光扫描法测定了金银花中绿原酸、异绿原酸A和异绿原酸C的含量,结果分别为2.76%、1.35%和0.733%。实验表明,金银花中各组分在硅胶板上能够完全分离,各组分在薄层板上性质稳定,测定精密度好,方法简单易行。
[Abstract]:Lv Yuan acid and isob_person1# acid are important active components of traditional Chinese medicine. They are widely used in medicinal plants. Determination of Lv Yuan acid and isob_person1# acid in Chinese traditional medicine samples is of great significance for the quality evaluation of traditional Chinese medicine. The sensitized fluorescence properties of iso#china_person0# acid B and isob_person1# acid C) were studied, and then the fluorescence analysis method of Lv Yuan acid and isob_person2# acid in chrysanthemum and honeysuckle flower was studied, and the Lv Yuan acid in honeysuckle flower was determined by thin layer absorption-scanning method. The contents of iso#china_person0# acid A and isob_person1# acid C. The paper includes three parts: 1. The fluorescence properties of Lv Yuan acid and three kinds of isob_person2# acid were studied. The endogenous fluorescence of Lv Yuan acid and iso#china1# acid aqueous solution was very weak. Addition of borax and cetyltrimethylammonium bromide (CTAB) can produce strong sensitized fluorescence. The maximum excitation / emission wavelength of the fluorescence peak of Lv Yuan is at 350 nm/460 nm. Iso#china_person0# acid A was at 350 nm/480 / nm, and iso#china_person1# acid B was at 350 nm/471nm. The sensitized fluorescence intensity of iso#china_person0# C at 350 nm/480 Nm. The sensitized fluorescence intensity of the four substances was in agreement with the volume fraction and ionic strength of methanol in aqueous solution. Under the optimum experimental conditions, the fluorescence quantum yield of Lv Yuan acid was 0.0177, the isob_person1# acid A was 0.0167, and the iso#china_person2# acid B was 0.0154. Iso#china_person0# acid C is 0.0248.2. Due to the very similar fluorescence properties of Lv Yuan acid and isob_person2# acid, it is difficult to determine the contents of various components without separation. Therefore, the total amount of Lv Yuan acid and isob_person1# acid in chrysanthemum and honeysuckle were determined by fluorescence spectrophotometry and three dimensional fluorescence second order correction method. The average values of the two methods for determination of chrysanthemum were 5.59% and 5.59%, respectively. 0%. The average values of the determination results of honeysuckle were 7.05% and 8.48.The results showed that the total amount of Lv Yuan acid and isob_person1# acid in chrysanthemum and honeysuckle flower could be determined by fluorescence analysis. Then it can be used to evaluate the quality of medicinal materials .3.The content of Lv Yuan acid, isobali_person1# acid A and iso#china_person2# acid C in Flos Lonicerae was determined by thin layer absorption-scanning method. The results were 2.76%, respectively. The experimental results show that the components of Flos Lonicerae can be completely separated on silica gel plate, the properties of each component are stable on the thin layer plate, the precision is good, and the method is simple and feasible.
【学位授予单位】：河北师范大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R284.1;O657.3

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