新疆哈萨克族阿尔茨海默病中医证型与ApoE基因多态性的相关性研究

发布时间：2018-01-06 14:40

本文关键词：新疆哈萨克族阿尔茨海默病中医证型与ApoE基因多态性的相关性研究　出处：《新疆医科大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的:探讨新疆哈萨克族阿尔茨海默病中医证型与ApoE基因多态性的相关性研究。方法:收集2010年6月-2016年11月在新疆阿勒泰地区完成AD流行病学调查的基础上诊断哈萨克族AD患者及新疆神经退行性疾病临床大数据库的AD患者共58例,对照组共79例,将病例组按中医诊断标准及常用证候分型标准进行分类,通过对各组人群ApoE基因型的检测,比较其分布的差异性。结果:通过本次实验研究,新疆哈萨克族人群两组基因型分布在AD患者中,ApoEε2携带者比例明显降低,ApoEε4携带者比例明显升高;在正常人群中,ApoEε3携带者比例最高,ApoEε2次之,ApoEε4最低。两组等位基因频率有显著差异性(P0.01)。中医分型后的ApoE基因型在病例组和对照组中的分布,肾精亏虚证ApoEε3/ε4基因型与对照组相比其分布较高(P0.05);与其他证型相比较亦有差异性(P0.05)。同时,rs429358T/C位点突变基因频率分布有统计学意义(P0.05),对于其它位点(rs769449、rs7412、rs405509)在现有的样本数上没有统计学意义(P0.05)。结论:通过本课题的研究,发现新疆哈萨克族阿尔茨海默病中医证型与ApoE基因多态性之间有相关性,其中rs429358T/C位点突变基因频率分布有统计学意义,提示该位点为新疆哈萨克族阿尔茨海默病的危险因素。
[Abstract]:Objective: to study the relationship between TCM syndromes of Alzheimer's disease and polymorphism of ApoE gene in Kazak nationality of Xinjiang. Methods:. The AD patients in Kazak nationality and the large clinical database of neurodegenerative diseases in Xinjiang were diagnosed on the basis of AD epidemiological investigation completed in Altay region of Xinjiang from June 2010 to November 2016. There were 58 cases. In the control group, 79 cases were classified according to the criteria of TCM diagnosis and common syndromes, and the genotypes of ApoE were detected in each group. Results: according to this experiment, the proportion of ApoE 蔚 2 carriers in two groups of Kazak population in Xinjiang was significantly lower than that in AD patients. The proportion of ApoE 蔚 4 carriers increased significantly. The proportion of ApoE 蔚 3 carriers was the highest in the normal population, followed by ApoE 蔚 2. ApoE 蔚 4 was the lowest. There was significant difference in allelic frequency between the two groups. The distribution of ApoE genotype after TCM typing was found in the case group and the control group. The distribution of ApoE 蔚 3 / 蔚 4 genotype in kidney essence deficiency syndrome was higher than that in control group (P 0.05). Compared with other syndromes, there was also a significant difference in the frequency distribution of mutation gene at rs429358T / C locus (P 0.05). For other loci, rs769449 / rs7412rs405509) there is no statistical significance in the number of existing samples. Conclusion: through the study of this subject. It was found that there was a correlation between the TCM syndrome type of Alzheimer's disease and the polymorphism of ApoE gene in Xinjiang Kazak nationality, and the frequency distribution of rs429358T/C locus mutation gene was statistically significant. These results suggest that this locus is a risk factor for Alzheimer's disease in Kazak nationality in Xinjiang.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R749.16

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