新疆地区维吾尔族心房颤动与胆固醇酯转运蛋白基因TaqIB多态性的关联研究

发布时间：2018-01-11 03:27

本文关键词：新疆地区维吾尔族心房颤动与胆固醇酯转运蛋白基因TaqIB多态性的关联研究　出处：《新疆医科大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的:心房颤动(房颤)是临床上最常见的心律失常之一。近年来,国内外房颤基因学方面的研究为明确房颤的发病机制提供了新的方向。本文探讨胆固醇酯转运蛋白基因(CTEP TaqIB)T-aqIB多态性与新疆地区维吾尔族心房颤动患者的关系,从而进一步明确房颤的分子生物学发病机制。方法:本研究采用病例对照方法,选取2014年2月—2015年10月新疆医科大学第一附属医院综合心脏内科收住的维吾尔族非瓣膜性房颤患者100例(房颤组),维吾尔族非房颤患者100例(对照组),从外周静脉血中提取基因组DNA,采用聚合酶链反应-限制性片段长度多态性法(PCR-PFLP)对CTEP基因进行多态性检测,比较2组人群基因多态性分布的差异,分析CTEP TaqIB基因型与房颤发病的相关性。结果:房颤组与对照组样本CTEP TaqIB基因多态性基因型符合哈迪-温伯格(Hardy-weinberg)平衡(房颤组χ~2=1.49,P=0.22;对照组χ~2=0.64,P=0.42)。CTEP TaqIB基因位点的测序结果显示其存在AA型、AG型、GG型3种基因型。基因型AA、AG、GG在维吾尔族房颤人群中分别有7例(7%)、48例(48%)、45例(45%),在对照组人群中分别有22例(22%)、54例(54%)、24例(24%),两组基因型和等位基因分布差异有统计学意义(χ~2=14.5,P㩳0.005;χ~2=13.5,P㩳0.005)。结论:新疆地区维吾尔族心房颤动的发生与CTEP TaqIB基因多态性有相关性。
[Abstract]:Objective: atrial fibrillation (AF) is one of the most common arrhythmias in clinic. Studies on the gene of atrial fibrillation at home and abroad have provided a new direction to clarify the pathogenesis of atrial fibrillation. This article discusses the cholesterol ester transporter gene CTEP TaqIBB). Relationship between T-aqIB polymorphism and Uygur patients with atrial fibrillation in Xinjiang. In order to further clarify the molecular pathogenesis of atrial fibrillation. Methods: this study adopted a case-control method. From February 2014 to October 2015, 100 Uygur patients with non-valvular atrial fibrillation (AF group) were enrolled in the Department of General Cardiology, first affiliated Hospital of Xinjiang Medical University. Genomic DNA was extracted from peripheral venous blood in 100 Uygur patients with non-atrial fibrillation (control group). Polymerase chain reaction-restriction fragment length polymorphism (PCR-PFLP) was used to detect the polymorphism of CTEP gene. The relationship between CTEP TaqIB genotype and atrial fibrillation was analyzed. Results: the polymorphism of CTEP TaqIB gene in atrial fibrillation group and control group was consistent with that of Hadi Weinberg (P < 0.05). Hardy-weinberg) balance (蠂 2 + 1.49 in AF group). P0. 22; The results of sequencing the TaqIB loci of the control group (蠂 ~ (2 +)) (0.64) showed that there were three genotypes of AA type AG and GG genotype. There were 7 cases of GG in Uygur patients with atrial fibrillation, 48 cases with GG and 45 cases with 45 cases with atrial fibrillation. In the control group, there were 22 cases (22 cases) and 54 cases (45 cases). There were significant differences in genotype and allele distribution between the two groups (蠂 2 = 14.5P? 0.005; 蠂 ~ 2 ~ (2) ~ (13. 5) P? Conclusion: the occurrence of atrial fibrillation in Uygur nationality is associated with the polymorphism of CTEP TaqIB gene.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R541.75

【参考文献】