let-7c-1对PTZ致痫大鼠学习记忆功能的影响

发布时间：2018-01-31 04:50

本文关键词： 癫痫 let-7c 学习记忆功能凋亡　出处：《广西医科大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的研究let-7c-1对戊四氮(pentylenetetrazol,PTZ)致痫大鼠学习记忆功能的影响,探讨其可能的机制。方法通过PTZ腹腔注射SD雄性大鼠建立慢性癫痫模型,随机分为癫痫组、干预对照组(侧脑室注射let-7c-1-3p agomir control)、let-7c-1激动剂组(侧脑室注射let-7c-1-3p agomir),每组12只,另设12只大鼠为正常对照组。28天后,观察各组大鼠的行为学变化,然后检测各组大鼠海马组织中let-7c-1基因表达量及Bcl-2蛋白、Caspase3蛋白的表达。结果1.行为学变化结果显示:与正常对照组相比,癫痫组大鼠逃避潜伏期延长、穿越平台次数减少、在目标象限的总路程缩短,差异有统计学意义(P0.05);癫痫组与干预对照组相比,逃避潜伏期、穿越平台次数、在目标象限的总路程无显著差异(P0.05);与干预对照组相比,let-7c-1激动剂组逃避潜伏期明显延长,穿越平台次数减少、在目标象限的总路程缩短,差异有统计学意义(P0.05)。2.let-7c-1基因表达:癫痫组let-7c-1基因表达量高于正常对照组组,差异有统计学意义(P0.05)。癫痫组与干预对照组相比无明显差异(P0.05)。let-7c-1激动剂组let-7c-1基因相对表达量明显高于干预对照组,差异有统计学意义(P0.05)。3.Bcl-2、Caspase3蛋白表达:与正常对照组相比,癫痫组的Bcl-2蛋白表达减少,Caspase3蛋白表达增加,差异有统计学意义(P0.05);癫痫组与干预对照组相比,Bcl-2蛋白及Caspase3蛋白的表达无显著差异(P0.05)。与干预对照组相比,let-7c-1激动剂组Bcl-2蛋白表达明显减少,Caspase3蛋白表达明显增加,差异有统计学意义(P0.05)。结论1.let-7c-1可使PTZ致痫大鼠的学习记忆功能受损。2.let-7c-1可能通过减少Bcl-2蛋白表达及增加Caspase-3蛋白表达增加神经细胞凋亡而使癫痫大鼠的学习记忆功能障碍加重。
[Abstract]:Objective to study the effects of let-7c-1 on learning and memory function in pentylenetetrazolium pentylenetetrazolium pentylenetetrazolium (PTZ) -induced epilepsy rats. Methods male SD rats were injected intraperitoneally with PTZ to establish chronic epilepsy model and were randomly divided into epileptic group. Intervention control group (intraventricular injection of let-7c-1-3p agomir control). Let-7c-1 agonist group (12 rats in each group) were injected with let-7c-1-3p agomirin in lateral ventricle, and 12 rats were used as normal control group for 28 days. The behavioral changes of rats in each group were observed, and then the expression of let-7c-1 gene and Bcl-2 protein in hippocampus of each group were detected. Results 1.Behavioral changes showed that compared with the normal control group, the escape latency of epileptic rats was prolonged, and the times of crossing the platform decreased. 2. The total distance in the target quadrant was shortened, and the difference was statistically significant (P 0.05). Compared with the intervention control group, the Epilepsy group had no significant difference in the total distance in the target quadrant (P 0.05). Compared with the intervention control group, the escape latency of the let-7c-1 agonist group was significantly prolonged, the number of times of crossing the platform was reduced, and the total distance in the target quadrant was shortened. The expression of let-7c-1 gene in epilepsy group was higher than that in normal control group. The difference was statistically significant (P 0.05). There was no significant difference between the epileptic group and the intervention control group (P 0.05). The relative expression of let-7c-1 gene in let-7c-1 agonist group was significantly higher than that in intervention control group. The difference was statistically significant (P 0.05). 3. Expression of Bcl-2Caspase3 protein: compared with normal control group, the expression of Bcl-2 protein in epileptic group was decreased. The expression of Caspase3 protein increased, and the difference was statistically significant (P 0.05). There was no significant difference in the expression of Bcl-2 protein and Caspase3 protein between the epileptic group and the intervention control group, but there was no significant difference between the epileptic group and the intervention control group. In let-7c-1 agonist group, the expression of Bcl-2 protein decreased significantly and the expression of Caspase3 protein increased significantly. The difference was statistically significant (P0.05). Conclusion 1. Let-7 c-1 can impair the learning and memory function of epileptic rats induced by PTZ. 2. Let-7 c-1 may decrease the expression of Bcl-2 protein and increase Caspase-3 eggs. 2. White expression increased neuronal apoptosis and aggravated learning and memory dysfunction in epileptic rats.
【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R742.1

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