刺络泻血对高尿酸血症模型大鼠血尿酸及相关酶活性的影响

发布时间：2018-02-02 17:03

  本文关键词： 刺络泻血 高尿酸血症 肝功能 黄嘌呤氧化酶 肾功能 血尿酸 腺苷脱氨酶　出处：《北京中医药大学》2017年硕士论文　论文类型：学位论文

【摘要】：刺络泻血,作为中医传统治疗方法之一,古谓"启脉"、"刺络",是指用三棱针或者其他泻血工具刺破人体穴位或周围血络,放出适量血液以达到防治疾病的目的的一种治疗方法。刺络泻血通过刺破体表瘀阻络脉,可以直接起到活血化瘀、疏通经络、祛瘀生新的功效。在临床上,经过长期的临床实践发现刺络泻血可以有效治疗高尿酸血症,降低血尿酸水平。前期做过刺络泻血治疗高尿酸血症的临床研究,在此基础上,本课题深入探讨刺络泻血治疗高尿酸血症的疗效及可能机制。本研究采用腺嘌呤联合乙胺丁醇灌胃法建立高尿酸血症大鼠模型,通过观"委中"、"足三里"、"丰隆"刺络泻血对高尿酸血症模型大鼠血尿酸、相关酶活性及肝肾功能的影响,以探讨刺络泻血治疗高尿酸血症的相关机制,为临床实践提供更多的依据。目的本实验采用腺嘌呤合乙胺丁醇混合溶液联合灌胃法,以制备高尿酸血症大鼠模型。造模成功后,刺络泻血组于委中穴、足三里穴及丰隆穴浅表血络行刺络泻血,别嘌呤醇组根据大鼠体重标准灌胃别嘌呤醇。治疗结束后,观察大鼠血清尿酸(serum uric acid,SUA)黄嘌呤氧化酶(xanthine oxidase,XOD)、腺苷脱氨酶(adenosine deaminase,ADA)、血肌酐(serumcreatinine,Cr)、血清尿素氮(blood urea nitrogen,BUN)、谷丙转氨酶(cer-ealthird transaminase,ALT)及谷草转氨酶(aspartate transaminase,AST)的影响。方法将雄性SD大鼠40只,按随机原则将大鼠分为正常组、模型组、别嘌呤醇组和刺络泻血组,每组各10只。造模第一阶段从实验开始,正常组根据体重标准按生理盐水350 mg.kg-1.d-1体重标准灌胃,其他组按腺嘌呤100 mg.kg-1.d-1和乙胺丁醇250 mg.kg-1.d-1体重标准灌胃,每天1次。连续4周造模成功后,第二阶段正常组按生理盐水350 mg.kg-1.d-1体重标准灌胃,每组大鼠仍按腺嘌呤100mg.kg-4.d-1体重标准灌胃,3次/周至第8周治疗结束停止,维持尿酸水平,以防止大鼠自愈。4周造模成功后,刺络泻血组选取大鼠双侧委中穴、足三里穴附近血络刺血,每次出血量约0.5-0.8ml,别嘌呤醇组据大鼠体重灌胃别嘌呤醇0.03 mg.kg-1.d-1,2次/周,至第8周治疗结束。治疗结束后,10%水合氯醛溶液腹腔注射麻醉大鼠,取血进行检测。采用比色法检测SUA、Cr、XOD、ADA,脲酶法检测BUN,丙酮酸氧化酶法检测ALT、AST。结果1.体质量、肾质量、肾指数:模型组、别嘌呤醇组、刺络泻血组大鼠的体质量均明显降低(P0.05);与正常组相比,大鼠肾质量、肾指数均明显升高;与模型组相比,别嘌呤醇组大鼠体质量、肾质量、肾指数均明显升高,刺络泻血组肾质量、肾指数均显著下降(P0.05);与别嘌呤醇组相比,刺络泻血组肾质量、肾指数均明显下降(P0.05)。2.血清SUA含量:与正常组相比,模型组SUA显著增高(P0.05),与模型组相比,别嘌呤醇组、刺络泻血组SUA含量显著降低(P0.05)。3.血清XOD、ADA活性情况:与正常组相比,模型组ADA、XOD活性均显著增高(P0.05),与模型组相比,别嘌呤醇组XOD活性显著降低(P0.05),刺络泻血组XOD、ADA活性显著降低(P0.05)。4.血清Cr、BUN含量:与正常组相比,模型组Cr、BUN含量均显著增高(P0.05);与模型组相比,别嘌呤醇组Cr、BUN含量无明显变化,刺络泻血组Cr、BUN含量降低(P0.05)。5.血清AST、ALT的变化:与正常组相比;ALT、AST活性明显增强(P0.05),与模型组相比,别嘌呤醇组AST活性无明显变化,ALT活性显著增强(P0.05),刺络泻血组ALT、AST活性显著降低(P0.05)。结论1.大鼠"委中"、"足三里"、"丰隆"刺络泻血可以降低高尿酸血症大鼠肾指数,减轻肾脏的充血、炎性反应。2.大鼠"委中"、"足三里"、"丰隆"刺络泻血可以有效抑制XOD及ADA活性,减少尿酸合成,降低大鼠SUA水平。3.大鼠"委中"、"足三里"、"丰隆"刺络泻血可以降低Cr、BUN含量及ALT、AST活性,在一定程度上可以降低尿酸对肝肾的损伤,同时也可以避免因服用降尿酸西药对肝肾的损伤。
[Abstract]:Pricking blood, as one of the traditional Chinese medicine treatment method, called "ancient kaiclock", "pricking", refers to the use of triangular needle or other tools on the acupoints of the human body spilled blood or peripheral blood vessels, a treatment method of releasing amount of blood to achieve the purpose of disease treatment. Blood pricking through puncture the surface of blood stasis in collaterals, can be directly to blood stasis, dredge the meridians, removing stasis and promoting new effect. In clinical practice, through long-term clinical practice found that blood pricking therapy can effectively reduce hyperuricemia, serum uric acid level. Early clinical research done pricking blood therapy, high urine acidemia, on the basis of on this topic, in-depth study of curative effect of pricking blood therapy of hyperuricemia and its possible mechanism. This study uses adenine and ethambutol to establish hyperuricemia rat model by intragastric administration, through the concept of "Wei", "Zusanli", "Fenglong" pricking blood of high blood uric acid A rat model with blood uric acid, related enzyme activity and liver function, the mechanism of pricking blood therapy of hyperuricemia, provide more evidence for clinical practice. The purpose of this experiment using adenine and ethambutol mixed solution combined gavage method, rat models with high uricemia. Successful model after pricking blood group in Weizhong, Zusanli and Fenglong superficial blood vessels to assassinate the winding spilled blood allopurinol group, according to the standard weight of rats with allopurinol. After the treatment, observation of serum uric acid in rats (serum uric acid SUA (xanthine) oxidase, xanthine oxidase XOD (adenosine deaminase), adenosine deaminase, ADA), serum creatinine (serumcreatinine, Cr), blood urea nitrogen (blood urea, nitrogen, BUN), alanine aminotransferase (cer-ealthird transaminase ALT) and aspartate aminotransferase (aspartate transaminase, AST). The effects of the method 40 male SD rats were randomly divide the rats into normal group, model group, allopurinol group and pricking blood group, 10 rats in each group. The first stage of modeling from the beginning of the experiment, the normal group according to the standard according to the weight of saline 350 mg.kg-1.d-1 weight standard intragastric administration, other groups by adenine 100 mg.kg-1.d-1 and 250 mg.kg-1.d-1 standard weight of ethambutol orally, 1 times a day. After 4 weeks, the second groups according to the stages of normal saline 350 mg.kg-1.d-1 weight gavage, the rats in each group according to the 100mg.kg-4.d-1 standard weight of adenine orally, 3 times / week to eighth weeks after the treatment stopped, maintain uric acid level in order to prevent the rat self-healing.4 weeks after the modeling, pricking blood group selected rats with bilateral Zusanli Point Weizhong blood pricking blood near, every time the amount of bleeding was about 0.5-0.8ml, allopurinol group according to the body weight of rats intragastric administration of allopurinol in 0.03 mg.kg-1.d-1,2 娆，

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