亚硝酸钠对雌性小鼠配子质量及生殖力影响的研究

发布时间：2018-02-09 00:14

  本文关键词： 亚硝酸钠 卵母细胞 氧化压力 生殖力 小鼠　出处：《内蒙古大学》2017年硕士论文　论文类型：学位论文

【摘要】：亚硝酸钠是一种工业化学制剂,常被作为食品添加剂用于肉制品的防腐,增加与保持色泽度。同时,许多地区地下水中亚硝酸盐与硝酸盐含量超标从而导致人们过量摄入。许多研究表明,摄入大剂量亚硝酸钠会增加癌症风险、导致高铁血红蛋白症、氧化应激甚至死亡。然而,亚硝酸盐对女性生育力或胎儿发育影响研究甚少。在我们的研究中,以5周龄的ICR品系雌性小鼠为实验对象,探究亚硝酸钠对雌性小鼠生殖力的影响。实验分为三组连续灌胃21天,分别为:不含有亚硝酸钠的生理盐水作为对照组,低剂量亚硝酸钠组60 mg/kg/天,高剂量亚硝酸钠组120 mg/kg/天。我们检测了灌胃后小鼠卵母细胞形态、纺锤体染色体复合体动力学、线粒体分布、三磷酸腺苷(adenosine triphosphate,ATP)水平、细胞早期凋亡、DNA损伤、组蛋白H3K4me2与H3K9me3修饰、活性氧(reactive oxygen species,ROS)水平、产仔情况。实验结果表明,亚硝酸钠导致GV期与MⅡ期卵母细胞数量减少,并抑制卵母细胞极体的排出,增加GV期卵母细胞透明带厚度。同时,亚硝酸钠引发了 MⅡ期卵母细胞纺锤体紊乱、线粒体分布异常、ATP水平降低、ROS水平升高、组蛋白H3K4me2甲基化水平上升、早期细胞凋亡增加和DNA损伤,更重要的是,亚硝酸钠的灌胃使雌性小鼠产仔数明显减少。综合上述实验结果,我们认为亚硝酸钠影响雌性小鼠的生殖机能,造成生殖毒性。这些数据为日后亚硝酸盐在食品和其他工业生产中的应用所引起的人类生殖障碍提供了理论基础。
[Abstract]:Sodium nitrite is a kind of industrial chemical preparation, which is often used as food additive for preservative of meat products to increase and maintain color. In many areas, excessive levels of nitrite and nitrate in groundwater lead to overconsumption. Many studies have shown that high doses of sodium nitrite increase the risk of cancer, lead to methemoglobin, oxidative stress and even death. The effects of nitrite on female fertility or fetal development were seldom studied. In our study, 5-week-old ICR strain female mice were used as experimental subjects. To explore the effect of sodium nitrite on the fecundity of female mice, the experiment was divided into three groups: normal saline without sodium nitrite as control group and low dose sodium nitrite group with 60 mg 路kg ~ (-1) 路kg ~ (-1) 路d ~ (-1) of sodium nitrite. In high dose sodium nitrite group (120 mg / kg / d), the morphology of oocytes, the dynamics of spindle chromosome complex, the distribution of mitochondria, adenosine triphosphate- ATPase level, early apoptosis and DNA damage, histone H3K4me2 and H3K9me3 modification were measured. The results showed that sodium nitrite reduced the number of oocytes in GV phase and M 鈪，

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