益母草药材产地加工与饮片炮制生产一体化工艺研究

发布时间：2018-03-05 02:02

本文选题：益母草　切入点：生物碱　出处：《南京中医药大学》2017年硕士论文　论文类型：学位论文

【摘要】：益母草为常用中药,历代本草均有收载,主要含有生物碱类、黄酮类、二萜类、挥发油类等化合物。药理研究表明,益母草有收缩子宫、抑制血小板聚集等广泛的作用。临床上主要用于月经不调、行经腹痛及产后瘀阻等症。益母草质地疏松,易于切制,但贮藏及运输不便,且有效成分易随水流失。通过将产地加工和炮制进行整和,可以通过减少水处理步骤,控制干燥的温度和时间,从而保证饮片的质量,建立、形成具有优化生产环节、便于贮藏运输、降低成本等优势的产地加工与炮制生产一体化的工艺技术和规范。根据益母草宜采用产地加工技术、传统炮制技术及产地加工与炮制一体化生产饮片的比较性研究。建立体现中药安全、有效及可控性的评价质量体系。本文运用现代的分析技术与方法,初步建立益母草中生物碱类、黄酮类、无机元素的分析与评价方法,探讨了不同干燥方式对益母草中的有效成份与无机元素的影响,运用网络药理学方法通过构建"药物-靶点-疾病"网络,全面系统预测益母草生物碱类成分的潜在作用靶点,分析其作用机制。为益母草质量标准的提升及生物活性的研究,提供科学合理的理论依据。生物碱类和黄酮类成分的分析:建立了益母草生物碱类与黄酮类成分的反相高效液相含量测定方法。以甲醇(A)-0.1%磷酸溶液(B)为流动相等度洗脱,流速控制在1.0mL·min-1，，柱温:30℃,在检测波长为270 nm的条件下,益母草碱、芦丁、槲皮素、芹菜素、芫花素均能够达到基线分离;以乙腈-水(80:20),流速:1.0mL·min-1,柱温:30℃,用蒸发光散射检测器检测,载气流速为2.4L·min-1,漂移管温度为80℃的条件下,可测定水苏碱的含量。根据不同产地益母草生物碱类和黄酮类成分的测定分析,建立益母草药材的高效液相指纹图谱。无机元素与重金属元素的分析:采用ICP-MS法简单、快速、有效的测定益母草中15种无机元素的含量,研究结果显示益母草中微量元素中以Ga、Ba、Cu、Zn的含量较为丰富。重金属Hg有少量检出外,其余重金属几乎均未检出且在2015年《中国药典》拟新增中药重金属的限量标准之内,通过主成分分析将Ga、Ba、Cu、Zn、Tl、Ni、Sn、As作为益母草中特征元素与质量评价的重要因子。干燥方式对益母草有效成分与无机元素的影响:探讨5种不同的干燥技术(包括传统的阴干、晒干与现代的热风干燥、红外干燥、微波干燥)对益母草中生物碱类、黄酮类含量以及无机元素的影响情况。结果显示,鲜益母草中生物碱类成分含量较高,而热风干燥处理后,益母草中的生物碱类和黄酮类成分保留最多;微波干燥对这两类有效成分造成的损失最大。而益母草干燥前后无机元素的含量变化明显,差异显著的元素主要是Mn,Zn,Sn,Ba,其中Mn,Zn的含量在干燥后增加,而Sn,Ba的含量在干燥后显著下降。文章以探讨生物碱类成分的潜在作用靶点和作用机制为目的,采用反向药效团匹配方法,结合生物信息学分析,预测了水苏碱和益母草碱的潜在作用靶点,并构建了生物碱成分-靶点-疾病网络,验证水苏碱和益母草碱是益母草的主要药理成分,并通过多靶点多途径共同发挥药理作用。研究发现,20个基因PCK1、PTpN1、OAT、CYP2C9、GSTP1、FGG、AKR1C3、SRC、CRABP2、HMGCR、GSTA1、PDE4D、GPI、SULT2B1、RASH、CA2、CBS、HSP71、GSTM2、LCN2、CDK2可能是两种生物碱共同的潜在重要作用靶点,Metabolic pathways、Biosynthesis of antibiotics 以及 Drug metabolism-cytochrome P450 等 7 种通路可能是两种生物碱发挥药理作用的重要途径。生物碱类成分的预测结果不仅体现了中药多靶点、多途径的作用特点,还为深入探讨其作用机制提供了理论依据。
[Abstract]:Motherwort is commonly used in traditional Chinese medicine, ancient herbal were recorded, mainly include alkaloids, flavonoids, two terpenes, volatile oils and other compounds. Pharmacological studies showed that motherwort has uterine contraction, inhibition of platelet aggregation. Widely used clinically for the irregular menstruation, abdominal pain and postpartum blood stasis syndrome. Motherwort loose texture, easy to cut, but the storage and transportation is inconvenient, and the effective components with water loss. The origin of the whole processing and through the processing steps to reduce the water control, drying temperature and time, quality, so as to ensure the establishment of form a pieces, optimize production processes, convenient storage and transportation. Reduce the cost advantage of producing and processing production integration technology and standard. Motherwort should adopt the origin processing technology based on the traditional processing technology, and the origin processing and system integration production pieces of cannon The comparative study of the Chinese medicine. The establishment of safe, effective and controllable quality assessment system. In this paper, analysis of the use of modern technology and methods, established, motherwort alkaloid flavonoids, analysis and evaluation methods of inorganic elements, discusses the influence of different drying methods on active ingredients and inorganic elements in motherwort. Using network pharmacology method through the construction of "network drug target disease", a comprehensive system to predict potential targets of motherwort alkaloids, analysis its mechanism. Research activity for the upgrading of quality standards and motherwort substances, provide a theoretical basis for scientific and reasonable. Analysis of alkaloids and flavonoids: establish the alkaloids and flavonoids in Herba Leonuri by reversed-phase HPLC method for the determination of methanol. (A) -0.1% phosphoric acid solution (B) as mobile phase elution, flow rate control in 1.0mL. Min-1, column temperature: 30 degrees, at the detection wavelength of 270 nm, Leonurine, rutin, quercetin, apigenin and genkwanin were able to reach the baseline separation; using acetonitrile and water (80:20), flow rate: 1.0mL - min-1, column temperature: 30 degrees Celsius, with evaporative light scattering detector, carrier the gas flow rate was 2.4L - min-1, the condition drift tube temperature of 80 degrees, can be used to determine the content of stachydrine. According to the analysis of different origin of alkaloids and flavonoids in Herba Leonuri, a high performance liquid chromatographic fingerprint of Herba Leonuri. Analysis of inorganic elements and heavy metal elements: the ICP-MS method is simple, fast effective, determination of the content of 15 kinds of inorganic elements in Herba Leonuri, the results showed that trace elements in Herba Leonuri by Ga, Ba, Cu, Zn content is rich. There is a small amount of heavy metal Hg detection, other heavy metals were not detected in 2015 and almost Chinese intends to add Chinese Pharmacopoeia > < heavily Within the limits of the genus through principal component analysis, Ga, Ba, Cu, Zn, Tl, Ni, Sn, As as an important factor in Leonurus feature elements and quality evaluation. The effects of drying methods on the effective components and inorganic elements of Herba Leonuri: explore 5 different drying technologies (including the traditional shade, dry and modern hot air drying, infrared drying, microwave drying) of alkaloids in Herba Leonuri, effects of flavonoids and inorganic elements. The results showed that the alkaloid content was higher in fresh motherwort, and hot air drying treatment, motherwort of alkaloids and flavonoids in microwave drying of the most retained; two kinds of active ingredients can cause significant loss. And the long before and after drying the contents of inorganic elements change obviously, significant differences between the elements are mainly Mn, Zn, Sn, Ba, Mn, Zn content increased, after drying and Sn, the content of Ba in the dry After decreased significantly. Potential targets and to explore the mechanism of the alkaloids role for the purpose of using the reverse pharmacophore matching method, combined with bioinformatics analysis, to predict the potential targets of stachydrine and Leonurine, and construct alkaloids target disease network, verification of stachydrine and Leonurine is a main pharmacological component of Herba Leonuri, and through multi targets more ways to exert their pharmacological effects. The study found that 20 genes PCK1, PTpN1, OAT, CYP2C9, GSTP1, FGG, AKR1C3, SRC, CRABP2, HMGCR, GSTA1, PDE4D, GPI, SULT2B1, RASH, CA2, CBS, HSP71. GSTM2, LCN2, CDK2 may be the two alkaloids common potentially important targets, Metabolic pathways, Biosynthesis of antibiotics and Drug metabolism-cytochrome P450 7 pathway may be an important way of two alkaloids. The pharmacological effects of alkaloids The prediction results not only reflect the characteristics of multiple targets and multiple pathways of Chinese medicine, but also provide a theoretical basis for the in-depth discussion of its mechanism of action.

【学位授予单位】：南京中医药大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R283;R284.1

【相似文献】