巨噬细胞标记物CD68和CD163在泡型肝包虫病人边缘带肝组织和血清中的表达及相关性分析

发布时间：2018-03-17 14:50

本文选题：泡型肝包虫病　切入点：分化群68分子　出处：《青海大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的:CD68和CD163是不同功能状态的巨噬细胞标记物,巨噬细胞被认为是其他寄生虫感染中的必需细胞。本研究旨在探讨泡型肝包虫病患者组织和外周血中CD68和CD163的表达情况,以及巨噬细胞在泡型肝包虫病发展中的作用。方法:通过免疫组织化学法检测术后泡型肝包虫病患者边缘带肝组织和正常肝组织CD68和CD163的表达,用ELISA法检测血清中s CD68和s CD163(CD68和CD163的可溶性形式)的浓度。通过Mann-Whitney检验评估CD68和CD163与患者特征的关系。通过线性回归分析评估组织和血清中CD68和CD163表达之间的关系。结果:边缘带肝组织CD68和CD163阳性细胞率均明显高于正常肝组织(60%vs.10%,85%vs.15%)。然而,边缘带肝组织CD68和CD163的表达差异无统计学意义(P0.05)。患者组s CD68和s CD163平均浓度(ng/ml)均高于健康对照组(11.36±3.46vs.2.57±2.72,209.73±186.40vs.86.34±135.80),患者组s CD163浓度明显高于s CD68组(P0.05)。此外,组织和血清中CD68和CD163水平都呈正相关(R2=0.896,P0.01;R2=0.491,P0.05)。结论:HAE患者病变边缘带肝组织CD68和CD163水平均显着升高,HAE患者血清中s CD68和s CD163水平也增加。巨噬细胞可能在泡型肝包虫病的发病机制中发挥作用。
[Abstract]:Objective to investigate the expression of CD68 and CD163 in the tissues and peripheral blood of patients with hepatic hydatid disease. Methods: immunohistochemical method was used to detect the expression of CD68 and CD163 in the marginal and normal liver tissues of patients with alveolar hydatid disease. The concentration of s CD68 and s CD163(CD68 and the soluble form of CD163 in serum were detected by ELISA method. The relationship between CD68 and CD163 and the characteristics of patients was evaluated by Mann-Whitney test. The correlation between CD68 and CD163 expression in tissue and serum was evaluated by linear regression analysis. Results: the positive rates of CD68 and CD163 in marginal liver tissue were significantly higher than those in normal liver tissue. There was no significant difference in the expression of CD68 and CD163 in the marginal liver tissue. The average concentrations of s CD68 and s CD163 in the patients group were higher than those in the healthy control group (11.36 卤3.46vs.2.57 卤2.72vs.209.73 卤186.40vs.86.34 卤135.80). In addition, the concentration of s CD163 in the patient group was significantly higher than that in the CD68 group (P 0.05). There was a positive correlation between the levels of CD68 and CD163 in tissues and serum. Conclusion the levels of CD68 and CD163 in the liver tissue of the lesion marginal zone in the patients with Hae were significantly higher than those in the controls. The levels of s CD68 and s CD163 in the serum of patients with HAE were significantly higher than those of the controls. Macrophages may be associated with hydatidosis of the alveolar liver. Play a role in the pathogenesis of the disease.
【学位授予单位】：青海大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R657.3

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