骨髓增生异常综合征患者FHIT、SOCS1、DAPK和P15基因甲基化的研究

发布时间：2018-03-18 22:47

本文选题：骨髓增生异常综合症　切入点：甲基化　出处：《新疆医科大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的:研究骨髓增生异常综合征(myelodysplastic syndrome,MDS)患者FHIT、SOCS1、DAPK和P15基因甲基化的表达及其临床意义。方法:采用甲基化特异性方法检测骨髓标本中FHIT、SOCS1、DAPK和P15基因的甲基化状态,分析甲基化状态与临床相关性。结果:67例MDS患者FHIT、SOCS1、DAPK和P15基因甲基化频率分别为52.2%(35/67)、47.8%(32/67)、35.8%(24/67)和37.3%(25/67),较对照组增高明显(P0.05);相对低危组与相对高危组患者相比较,P15、SOCS1甲基化频率明显增高(P0.05);四种基因中高于2个基因甲基化率对于相对高危组明显高于相对低危组患者(P0.05);67例患者中位生存时间为18(13.3-22.7)月;基因甲基化阳性患者生存时间明显短于阴性患者(15个月与21个月,P0.05);相对低危组中SOCS1基因甲基化患者生存时间短于非甲基化患者(P0.05)。相对高危组中SOCS1、P15及≥2个基因甲基化患者生存时间明显短于非甲基化患者(P0.05)。多因素分析显示SOCS1及P15基因甲基化是MDS患者预后不良的影响因素。结论:FHIT、SOCS1、DAPK和P15基因甲基化在MDS中具有一定的影响;SOCS1、P15基因甲基化是MDS患者预后不良的影响因素。
[Abstract]:Objective: To study the myelodysplastic syndrome (myelodysplastic syndrome, MDS) in patients with FHIT, SOCS1, DAPK expression and methylation of P15 gene and its clinical significance. Methods: using methyl FHIT, specific method for the detection of SOCS1 in bone marrow samples, methylation of DAPK gene and P15 gene, methylation status and clinical correlation analysis. Results: 67 cases of MDS patients with FHIT, SOCS1, DAPK and P15 gene methylation frequencies were 52.2% (35/67), 47.8% (32/67), 35.8% (24/67) and 37.3% (25/67), significantly higher than the control group (P0.05); patients with relatively low risk group and the relatively high risk group compared to P15, SOCS1 methyl the frequency increased significantly (P0.05) higher than 2; gene methylation rate for the relatively high risk group was significantly higher than the relatively low risk group were four (P0.05) gene in 67 patients; the median survival time was 18 months (13.3-22.7); gene methylation positive patients had shorter survival time than those with Who (15 months and 21 months, P0.05); relative survival time in patients with SOCS1 gene methylation in the low risk group is shorter than the patients without methylation (P0.05). The relatively high risk group of SOCS1, P15 and more than 2 gene methylation in patients with survival time was shorter in patients with non methylation (P0.05). Factor analysis showed that SOCS1 and P15 gene methylation is the influencing factors of poor prognosis of patients with MDS. Conclusion: FHIT, SOCS1, DAPK and methylation of P15 gene has a certain effect in MDS; SOCS1, P15 gene methylation is the influencing factors of poor prognosis of patients with MDS.

【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R551.3

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