荷青花中酚类成分的研究

发布时间：2018-04-20 21:28

本文选题：荷青花 + 酚类　；参考：《吉林大学》2017年硕士论文

【摘要】：研究背景:荷青花[Hylomecon japonica(Thunb.)Prantl et Kündig]为荷青花属(Hylomecon)植物,别名鸡蛋黄花(东北)、拐枣七、刀豆三七、水菖三七,大叶老鼠七等,属于多年生早春类短命植物,主要分布于我国黑龙江、吉林、辽宁、陕西、四川、安徽、浙江、湖南、湖北及山西南部等地,在日本、朝鲜及俄罗斯西伯利亚等地也有分布。生于海拔300-1800(2400)米的落叶阔叶林下、杂木林下、林缘、沟边或者沙地,喜水肥条件较好的腐殖土和适当遮阴的环境。荷青花多以根茎入药,具有祛风除湿,舒筋通络,散瘀消肿,止血镇痛之效。荷青花常与多种中药合用,用于治疗风寒湿痹、风湿关节痛、跌打损伤、劳伤、四肢乏力、胃脘痛、痢疾等疾病。实验目的:查阅文献发现,有关荷青花成分的研究很少,从中分离出的化合物主要为生物碱类,而对其它成分的研究目前尚无报道。荷青花属中的其他植物主要分离出酚类及萜类,整体研究尚不深入。现代药理学研究表明,荷青花属植物抗菌、抗炎作用显著。为更好的开发利用荷青花属植物荷青花的药用价值,从而为中药现代化奠定坚实的基础,本文对荷青花中酚类成分进行了系统的研究。实验方法:取荷青花全草,用70%的乙醇溶液重复冷浸提4次,每次一周。合并提取液,减压回收乙醇后合并水层,干燥后用水溶解,过滤除去水不溶性杂质,上样于已处理好的D101大孔树脂,依次用0%、10%、30%、50%、70%、95%的乙醇洗脱,收集30%部分,干燥后用水溶,除去水不溶性杂质,剩余样品经正相硅胶柱分离得到A、B、C、D四部分,利用大孔树脂柱层析、正相硅胶柱层析、HPLC半制备等手段分离得到五个单体化合物。实验结果:利用上述方法,从荷青花中分离得到4个酚类化合物和1个单萜类化合物,并鉴定其结构分别为:I:香叶木素-7-O-β-D-吡喃木糖基(1→6)-β-D-吡喃葡萄糖苷II:苯甲基-α-L-吡喃阿拉伯糖基(1→6)-β-D-吡喃葡萄糖苷III:苯甲基-β-D-吡喃木糖基(1→6)-β-D-吡喃葡萄糖苷IV:苯乙基-α-L-吡喃阿拉伯糖基(1→6)-β-D-吡喃葡萄糖苷V:3-羰基-6-羟基-紫罗兰酮实验结论:化合物I-IV为荷青花植物中首次得到,V为荷青花属中首次得到。
[Abstract]:Background: Hylomecon japonica(Thunb.)Prantl et K 眉 ndig is a species of Hylomecon, also known as Hylomecon, also known as Hylomecon, also known as Hylomecon, also known as Hylomecon, also known as Hylomecon, also known as Hylomecon, also known as Hylomecon, also known as Hylomecon, also known as Hylomecon, also known as Hylomecon, also known as Hylomecon. Jilin, Liaoning, Shaanxi, Sichuan, Anhui, Zhejiang, Hunan, Hubei and southern Shanxi are also distributed in Japan, Korea, Russia and Siberia. Under deciduous broad-leaved forest with 300-1800 m above sea level, under miscellaneous forest, edge of forest, edge of forest or sandy land, humic soil with better conditions of water and fertilizer and suitable shading environment. Lotus blue and white to root into medicine, with dispelling wind dehumidification, Shujin Tongluo, disperse blood stasis and detumescence, hemostatic analgesia effect. It is often used in the treatment of rheumatism and dampness arthralgia, falling injury, labor injury, limb fatigue, epigastric pain, dysentery and other diseases. Objective: to find out that there are few studies on the components of lotus blue and white flowers, and the compounds isolated from them are mainly alkaloids, but there are no reports on the other constituents. Phenols and terpenoids are mainly isolated from other plants in the genus Salvia. Modern pharmacological studies have shown that the plants of the genus Salvia are antibacterial and have significant anti-inflammatory effects. In order to better develop and utilize the medicinal value of lotus blue and white plants and lay a solid foundation for the modernization of traditional Chinese medicine, the phenolic components in lotus blue and white flowers were systematically studied in this paper. Methods: the whole plant was extracted with 70% ethanol solution for 4 times, one week each time. Combine the extract, decompress and recover the ethanol and merge the water layer, dissolve with water after drying, filter out the water insoluble impurities, apply the sample to the treated D101 macroporous resin, then use 010, 1010, 30 and 30% of ethanol to be eluted, collect 30% of the water, and dissolve with water after drying, After the water insoluble impurity was removed, the remaining samples were separated by normal phase silica gel column to obtain four parts of Agna Bu Con D, and five monomers were separated by macroporous resin column chromatography and HPLC semi-preparation by normal phase silica gel column chromatography. Results: four phenolic compounds and one monoterpenoid compound were isolated from the blue and white flowers by the method mentioned above. Their structures were identified as: 1: I: vanillin-7-O- 尾 -Dpyranoxylycosyl 1 (1)-尾 -Dpyranoside (II): phenylmethyl- 伪 -L-pyrrolidene arabinoside (1)-尾 -glucopyranoside (III): benzomethyl- 尾 -D- pyranoxylycoside (1)-尾 -Dpyranoside (IV-1). Experimental results of phenylethyl- 伪 -L-pyrrolidene arabinyl glycosyl 1 and 尾 -Dpyranoside (V: 3-carbonyl -6-hydroxy-ionone) conclusion: the compound I-IV is the first obtained from the plants of the genus Salvia.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R284.1

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