微等离子体射频对兔耳增生性瘢痕微血管数量及IL-8表达的影响

发布时间：2018-05-05 13:32

本文选题：微等离子体射频技术 + 增生性瘢痕　；参考：《河北医科大学》2017年硕士论文

【摘要】：目的:微等离子体射频技术作为一种治疗瘢痕的新技术已在临床上广为应用,但对于早期增生性瘢痕的治疗研究较少,本实验通过研究微等离子体射频技术治疗兔耳增生性瘢痕组织后白细胞介素-8(Interleukin 8,IL-8)的表达变化与微血管计数(Microvessel Count,MVC)的关系,评价微等离子体射频技术对早期增生性瘢痕的治疗效果,为微等离子体射频技术治疗早期增生性瘢痕提供实验依据及理论基础。方法:1选择6只同属新西兰大耳兔,全部为雌兔,体重2.5~3kg。在兔耳腹侧面建立增生性瘢痕的模型,造模成功后将每只兔子的双耳随机分配到实验组和对照组,实验组应用微等离子体射频技术治疗,对照组未予治疗。治疗30天后切取实验组和对照组增生性瘢痕组织制作标本,对标本进行固定、脱水、浸石蜡、包埋,切片,切片厚度为4μm。切片采用HE染色及显微镜观察各实验组与对照组兔耳增生性瘢痕的组织学变化,利用CD34内皮细胞抗原标记血管内皮细胞显示瘢痕内微血管密度变化,采用免疫组织化学染色检测瘢痕内IL-8表达的变化。2统计各治疗组与对照组兔耳增生性瘢痕组织中微血管密度及趋化因子IL-8的平均光密度值,数据用均数±标准差表示。应用SPSS 21.0进行配对设计的t检验及相关性分析,以P0.01作为有无显著性差异的标准。结果:1组织形态学观察结果对照组:肉眼可见瘢痕组织突出于皮肤表面,颜色发红、质地硬;HE染色光镜下可见真皮层增厚,微血管数量较多,成纤维细胞及胶原纤维大量增生,排列不规则。实验组:肉眼可见瘢痕组织高度较对照组变平,颜色变淡、质地变软;HE染色光镜下可见真皮层厚度变薄,瘢痕组织内微血管的数量较对照组明显减少,成纤维细胞数目减少,胶原纤维束较治疗前排列整齐。2免疫组化结果2.1实验组微血管密度为27.00±5.49个/mm2,对照组微血管密度为48.25±8.51个/mm2,两组比较差异具有统计学意义(P0.01),表明实验组微血管密度较对照组微血管密度明显减少。2.2实验组增生性瘢痕中IL-8的平均光密度值为0.016±0.011,对照组IL-8的平均光密度值为0.078±0.023,两组比较差异具有统计学意义(P0.01),表明实验组IL-8的表达低于对照组。2.3增生性瘢痕中微血管计数与IL-8的表达呈密切正相关(P0.01)。结论:微等离子体射频技术对于兔耳增生性瘢痕有一定防治作用;抑制增生性瘢痕组织内血管生成及IL-8趋化因子的表达可能是预防和治疗增生性瘢痕的新方法。
[Abstract]:Objective: as a new technique for the treatment of scar, microplasma radiofrequency technique has been widely used in clinic, but there is little research on the treatment of early hypertrophic scar. The purpose of this study was to investigate the relationship between the expression of interleukin-8 (IL-8) and microvessel count (MVCC) in rabbit ear hypertrophic scar treated with microplasma radiofrequency (RF) technique, and to evaluate the therapeutic effect of microplasma radiofrequency technique on early hypertrophic scar. To provide experimental and theoretical basis for the treatment of early hypertrophic scar by microplasma radiofrequency technique. Methods six New Zealand big eared rabbits, all of them female, were selected. The hypertrophic scar model was established on the ventral side of the rabbit ear. After the establishment of the model, each rabbit's ears were randomly assigned to the experimental group and the control group. The experimental group was treated with microplasma radiofrequency technique, but the control group was not treated. After 30 days of treatment, the experimental group and the control group were cut out to make specimens of hypertrophic scar. The specimens were fixed, dehydrated, soaked in paraffin, embedded, sliced, and the thickness of sections was 4 渭 m. The histopathological changes of hypertrophic scar were observed by HE staining and microscope in each experimental group and control group. Vascular endothelial cells labeled with CD34 endothelial cell antigen were used to show the change of microvessel density in the scar. Immunohistochemical staining was used to detect the expression of IL-8 in hypertrophic scar. 2 the mean optical density of microvessel density and chemokine IL-8 in hypertrophic scar tissue of rabbit ear were measured by means of mean 卤standard deviation. T test and correlation analysis of pairing design were carried out with SPSS 21.0, and P0.01 was taken as the criterion of significant difference. Results in the control group, the scar tissue was prominent on the skin surface, the color was red, the dermis was thickened, the number of microvessels was more, and the fibroblasts and collagen fibers were proliferating under light microscope. The arrangement is irregular. In the experimental group, the scar tissue was flattened, the color became lighter, the texture softened, the thickness of the dermis became thinner under HE staining, the number of microvessels in the scar tissue was significantly lower than that in the control group, and the number of fibroblasts was decreased. Collagen bundles were arranged neatly compared with before treatment by immunohistochemistry 2.1 the microvessel density of experimental group was 27.00 卤5.49 / mm ~ (-2), while that of control group was 48.25 卤8.51 / mm ~ (2). The difference between the two groups was statistically significant (P 0.01), which indicated that the microvessel density of experimental group was higher than that of control group (P < 0.05). The average optical density of IL-8 in hypertrophic scar was 0.016 卤0.011 in the control group and 0.078 卤0.023 in the control group. The difference between the two groups was statistically significant, indicating that the expression of IL-8 in the experimental group was lower than that in the control group. The microvessel count in hypertrophic scar was positively correlated with the expression of IL-8 in control group (P 0.01). Conclusion: microplasma radiofrequency technique can prevent and treat hypertrophic scar in rabbit ear and inhibit angiogenesis and expression of IL-8 chemokine in hypertrophic scar tissue may be a new method to prevent and treat hypertrophic scar.
【学位授予单位】：河北医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R622

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