蛋白质组学技术在流感病毒与宿主细胞相互作用研究中的应用

发布时间：2018-05-05 16:43

本文选题：流感病毒 + 蛋白质组学　；参考：《吉林大学》2017年硕士论文

【摘要】：流感病毒是流行性感冒(流感)的病原体。其中甲型流感病毒的抗原性易发生变异,已经多次引起世界性大流行。对禽流感病毒的重视源于1918年到1919年之间在全球杀死5000万到1亿人的西班牙大流感。正是由于这种灾难性的事情一次次出现,激发了广大的国内外专家学者对其进行研究的浓厚兴趣,科研工作者们前赴后继争相研究流感病毒的奥秘。本学位论文充分利用了蛋白质组学技术,详细研究了流感病毒与宿主细胞的相互作用,在流感病毒侵染Vero细胞的实验中,找到15个新表达的差异蛋白,发现了其特异性表达的蛋白质翻译后修饰。在对三组样品进行液相-质谱联用分析后发现了更多的差异,找到上调蛋白36个,下调蛋白19个。而在棕榈酰化修饰的实验中,又对流感病毒的蛋白进行了分析鉴定,结果发现利用酰基-生物素置换反应、亲和层析和质谱技术,鉴定出A型流感病毒血凝素蛋白胞质尾区的两个棕榈酰化位点(Cys562和Cys565)。也就是在这两个位点上发生了脂质共价修饰。正是由于棕榈酰化可以涉及病毒生命周期的多个阶段,特别是初始阶段和最终阶段,才使得A型流感病毒血凝素蛋白S-棕榈酰化修饰的研究被赋予了重要的意义。本实验的研究也为完善流感病毒感染机制及发现新的作用靶点提供了相应的信息。
[Abstract]:Influenza virus is the pathogen of influenza. The antigenicity of influenza A virus is easy to mutate and has caused worldwide pandemic many times. The emphasis on avian influenza virus stems from the Spanish pandemic that killed 50 million to 100 million people worldwide between 1918 and 1919. It is precisely because this kind of catastrophic thing appears time after time, has aroused the domestic and foreign experts and scholars to carry on the research intense interest, the scientific research worker goes on to study the influenza virus mystery one after another. In this dissertation, the interaction between influenza virus and host cell was studied in detail by using proteomics technique. In the experiment of influenza virus infecting Vero cells, 15 new differentially expressed proteins were found. It was found that the protein expressed specifically was modified after translation. Three groups of samples were analyzed by liquid phase mass spectrometry and 36 up-regulated proteins and 19 down-regulated proteins were found. In the experiment of palmitoacylation modification, the protein of influenza virus was analyzed and identified. The results showed that using acyl-biotin replacement reaction, affinity chromatography and mass spectrometry, Two palmitoacylation sites, Cys562 and Cys565, were identified in the cytoplasmic tail region of hemagglutinin protein of influenza A virus. In other words, lipid covalent modification occurs at these two sites. It is precisely because palm acylation can involve many stages of the virus life cycle, especially the initial stage and the final stage, that the study on the modification of hemagglutinin protein S- palm acylation of influenza A virus is of great significance. This study also provides information for the improvement of influenza virus infection mechanism and the discovery of new targets.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R373

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